Lipidomics detection of brain cardiolipins in plasma is associated with outcome after cardiac arrest
Anthonymuthu T. S., Kenny E. M., Lamade A. M., Gidwani H., Krehel N. M., Misse A., Gao X., Amoscato A. A., Straub A. C., Kagan V. E., Dezfulian C., Bayır H.
Critical Care Medicine
Vol.47, Issue4, P. E292-E300
Опубликовано: 2019
Тип ресурса: Статья
DOI:10.1097/CCM.0000000000003636
Аннотация:
Objectives: Brain mitochondrial dysfunction limits neurologic recovery after cardiac arrest. Brain polyunsaturated cardiolipins, mitochondria-unique and functionally essential phospholipids, have unprecedented diversification. Since brain cardiolipins are not present in plasma normally, we hypothesized their appearance would correlate with brain injury severity early after cardiac arrest and return of spontaneous circulation. Design: Observational case-control study. Setting: Two medical centers within one city. Participants (Subjects): We enrolled 41 adult cardiac arrest patients in whom blood could be obtained within 6 hours of resuscitation. Two subjects were excluded following outlier analysis. Ten healthy subjects were controls. Sprague-Dawley rats were used in asphyxial cardiac arrest studies. Interventions: None. Measurements and Main Results: We developed a high-resolution liquid chromatography/mass spectrometry method and determined cardiolipins speciation in human brain, hear
Ключевые слова:
Global ischemia-reperfusion; Lipidomics; Mitochondrial dysfunction; Plasma cardiolipin; Prognostic biomarker
cardiolipin; cardiolipin; animal experiment; animal model; Article; brain injury; case control study; clinical article; controlled study; disease association; heart arrest; heart infarction; human; human tissue; injury severity; lipid blood level; lipidomics; nonhuman; observational study; priority journal; rat; resuscitation; return of spontaneous circulation; systemic circulation; animal; blood; cardiomyopathy; female; heart arrest; heart mitochondrion; male; mass fragmentography; metabolism; oxidation reduction reaction; procedures; Sprague Dawley rat; Animals; Brain Injuries; Cardiolipins; Cardiomyopathies; Cardiopulmonary Resuscitation; Case-Control Studies; Female; Gas Chromatography-Mass Spectrometry; Heart Arrest; Humans; Male; Mitochondria, Heart; Oxidation-Reduction; Rats; Rats, Sprague-Dawley
Язык текста: Английский
ISSN: 1530-0293
Anthonymuthu T. S.
Kenny E. M.
Lamade A. M.
Gidwani H.
Krehel N. M.
Misse A.
Gao X.
Amoscato A. A.
Straub A. C.
Kagan V. E. Valerian E 1946-
Dezfulian C.
Bayır H.
Антхонyмутху Т. С.
Кеннy Е. М.
Ламаде А. М.
Гидwани Х.
Крехел Н. М.
Миссе А.
Гао Х.
Амосcато А. А.
Страуб А. C.
Каган В. Е. Валериан Е 1946-
Дезфулиан C.
Байıр Х.
Lipidomics detection of brain cardiolipins in plasma is associated with outcome after cardiac arrest
Текст визуальный непосредственный
Critical Care Medicine
Lippincott Williams & Wilkins
Vol.47, Issue4 P. E292-E300
2019
Статья
Global ischemia-reperfusion Lipidomics Mitochondrial dysfunction Plasma cardiolipin Prognostic biomarker
cardiolipin cardiolipin animal experiment animal model Article brain injury case control study clinical article controlled study disease association heart arrest heart infarction human human tissue injury severity lipid blood level lipidomics nonhuman observational study priority journal rat resuscitation return of spontaneous circulation systemic circulation animal blood cardiomyopathy female heart arrest heart mitochondrion male mass fragmentography metabolism oxidation reduction reaction procedures Sprague Dawley rat Animals Brain Injuries Cardiolipins Cardiomyopathies Cardiopulmonary Resuscitation Case-Control Studies Female Gas Chromatography-Mass Spectrometry Heart Arrest Humans Male Mitochondria, Heart Oxidation-Reduction Rats Rats, Sprague-Dawley
Objectives: Brain mitochondrial dysfunction limits neurologic recovery after cardiac arrest. Brain polyunsaturated cardiolipins, mitochondria-unique and functionally essential phospholipids, have unprecedented diversification. Since brain cardiolipins are not present in plasma normally, we hypothesized their appearance would correlate with brain injury severity early after cardiac arrest and return of spontaneous circulation. Design: Observational case-control study. Setting: Two medical centers within one city. Participants (Subjects): We enrolled 41 adult cardiac arrest patients in whom blood could be obtained within 6 hours of resuscitation. Two subjects were excluded following outlier analysis. Ten healthy subjects were controls. Sprague-Dawley rats were used in asphyxial cardiac arrest studies. Interventions: None. Measurements and Main Results: We developed a high-resolution liquid chromatography/mass spectrometry method and determined cardiolipins speciation in human brain, hear