Impact of CD14 on Reactive Oxygen Species Production from Human Leukocytes Primed by Escherichia coli Lipopolysaccharides
Kabanov D. S., Vwedenskaya O. Y., Fokina M. A., Morozova E. M., Grachev S. V., Prokhorenko I. R.
Oxidative Medicine and Cellular Longevity
Vol.2019, Num.6043245
Опубликовано: 2019
Тип ресурса: Статья
Аннотация:
Lipopolysaccharides (LPS) from Gram-negative bacteria prime human polymorphonuclear neutrophils (PMNs) via multicomponent receptor cluster including CD14 and MD-2·TLR4 for the enhanced release of reactive oxygen species (ROS) were triggered by bacterial derived peptide N-formyl-methionyl-leucyl-phenylalanine (fMLP). In this study, we investigated the impact of CD14 on LPS-induced priming of human PMNs for fMLP-triggered ROS generation (respiratory or oxidative) burst. Monoclonal antibodies against human CD14 (mAbs) as well as isotype-matched IgG2a did not influence significantly fMLP-triggered ROS production from LPS-unprimed PMNs. Anti-CD14 mAbs (clone UCHM-1) attenuated LPS-induced priming of PMNs as it had been mirrored by fMLP-triggered decrease of ROS production. Similar priming activity of S-LPS or Re-LPS from Escherichia coli for fMLP-triggered ROS release from PMNs was found. Obtained results suggest that glycosylphosphatidylinositol-anchored CD14 is the key player in LPS-induc
Ключевые слова:
Amino acids; Cell membranes; Escherichia coli; Monoclonal antibodies; Oxygen; Cardio-vascular disease; Glycosylphosphatidylinositol; Gram-negative bacteria; Human leukocytes; Lipopolysaccharides; Multicomponents; Polymorphonuclear neutrophils; Reactive oxygen species; Polysaccharides; CD14 antigen; Escherichia coli lipopolysaccharide; formylmethionylleucylphenylalanine; immunoglobulin F(ab) fragment; immunoglobulin G2a; lipopolysaccharide; reactive oxygen metabolite; lipopolysaccharide receptor; reactive oxygen metabolite; Article; cell isolation; Escherichia coli; human; human cell; inflammation; leukocyte; neutrophil; oxidative stress; respiratory burst; genetics; metabolism; oxidative stress; Amino Acids; Impact; Oxygen; Polysaccharides; Priming; Production; Release; Surfaces; Escherichia coli; Humans; Leukocytes; Lipopolysaccharide Receptors; Lipopolysaccharides; Oxidative Stress; Reactive Oxygen Species
Язык текста: Английский
ISSN: 1942-0994
Kabanov D. S.
Vwedenskaya O. Y. O.Yu.
Fokina M. A. Marina Anatolyevna 1961-
Morozova E. M.
Grachev S. V. Sergej Vitalyevich 1948-
Prokhorenko I. R.
Кабанов Д. С.
Вwеденскайа О. Y. О.Ю.
Фокина М. А. Марина Анатольевна 1961-
Морозова Е. М.
Грачев С. В. Сергей Витальевич 1948-
Прохоренко И. Р.
Impact of CD14 on Reactive Oxygen Species Production from Human Leukocytes Primed by Escherichia coli Lipopolysaccharides
Текст визуальный непосредственный
Oxidative Medicine and Cellular Longevity
Vol.2019 Num.6043245
2019
Статья
Amino acids Cell membranes Escherichia coli Monoclonal antibodies Oxygen Cardio-vascular disease Glycosylphosphatidylinositol Gram-negative bacteria Human leukocytes Lipopolysaccharides Multicomponents Polymorphonuclear neutrophils Reactive oxygen species Polysaccharides CD14 antigen Escherichia coli lipopolysaccharide formylmethionylleucylphenylalanine immunoglobulin F(ab) fragment immunoglobulin G2a lipopolysaccharide reactive oxygen metabolite lipopolysaccharide receptor reactive oxygen metabolite Article cell isolation Escherichia coli human human cell inflammation leukocyte neutrophil oxidative stress respiratory burst genetics metabolism oxidative stress Amino Acids Impact Oxygen Polysaccharides Priming Production Release Surfaces Escherichia coli Humans Leukocytes Lipopolysaccharide Receptors Lipopolysaccharides Oxidative Stress Reactive Oxygen Species
Lipopolysaccharides (LPS) from Gram-negative bacteria prime human polymorphonuclear neutrophils (PMNs) via multicomponent receptor cluster including CD14 and MD-2·TLR4 for the enhanced release of reactive oxygen species (ROS) were triggered by bacterial derived peptide N-formyl-methionyl-leucyl-phenylalanine (fMLP). In this study, we investigated the impact of CD14 on LPS-induced priming of human PMNs for fMLP-triggered ROS generation (respiratory or oxidative) burst. Monoclonal antibodies against human CD14 (mAbs) as well as isotype-matched IgG2a did not influence significantly fMLP-triggered ROS production from LPS-unprimed PMNs. Anti-CD14 mAbs (clone UCHM-1) attenuated LPS-induced priming of PMNs as it had been mirrored by fMLP-triggered decrease of ROS production. Similar priming activity of S-LPS or Re-LPS from Escherichia coli for fMLP-triggered ROS release from PMNs was found. Obtained results suggest that glycosylphosphatidylinositol-anchored CD14 is the key player in LPS-induc