Genetic re-engineering of polyunsaturated phospholipid profile of Saccharomyces cerevisiae identifies a novel role for Cld1 in mitigating the...
Lou W., Ting H. -., Reynolds C. A., Tyurina Y. Y., Tyurin V. A., Li Y., Ji J., Yu W., Liang Z., Stoyanovsky D. A., Anthonymuthu T. S., Frasso M. A., Wipf P., Greenberger J. S., Bayır H., Kagan V. E., Greenberg M. L.
Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids
Vol.1863, Issue10, P. 1354-1368
Опубликовано: 2018
Тип ресурса: Статья
DOI:10.1016/j.bbalip.2018.06.016
Аннотация:
Cardiolipin (CL) is a unique phospholipid localized almost exclusively within the mitochondrial membranes where it is synthesized. Newly synthesized CL undergoes acyl remodeling to produce CL species enriched with unsaturated acyl groups. Cld1 is the only identified CL-specific phospholipase in yeast and is required to initiate the CL remodeling pathway. In higher eukaryotes, peroxidation of CL, yielding CLOX, has been implicated in the cellular signaling events that initiate apoptosis. CLOX can undergo enzymatic hydrolysis, resulting in the release of lipid mediators with signaling properties. Our previous findings suggested that CLD1 expression is upregulated in response to oxidative stress, and that one of the physiological roles of CL remodeling is to remove peroxidized CL. To exploit the powerful yeast model to study functions of CLD1 in CL peroxidation, we expressed the H. brasiliensis Δ12-desaturase gene in yeast, which then synthesized poly unsaturated fatty acids(PUFAs) that a
Ключевые слова:
cardiolipin; Cld1; lipid peroxidation; mass spectrometry (MS); polyunsaturated fatty acid (PUFA); remodeling; yeast
cardiolipin; cardiolipin d1; phospholipid; polyunsaturated fatty acid; unclassified drug; acyl coenzyme A desaturase; cardiolipin; CLD1 protein, S cerevisiae; delta-12 fatty acid desaturase; phospholipase; plant protein; Saccharomyces cerevisiae protein; unsaturated fatty acid; apoptosis; Article; genetic engineering; Hevea brasiliensis; hydrolysis; lipid peroxidation; mitochondrial membrane potential; nonhuman; oxidative stress; priority journal; protein expression; Saccharomyces cerevisiae; signal transduction; upregulation; chemistry; enzymology; genetics; growth, development and aging; Hevea; liquid chromatography; mass spectrometry; metabolism; procedures; Saccharomyces cerevisiae; Cardiolipins; Chromatography, Liquid; Fatty Acid Desaturases; Fatty Acids, Unsaturated; Genetic Engineering; Hevea; Hydrolysis; Lipid Peroxidation; Mass Spectrometry; Oxidative Stress; Phospholipases; Plant Proteins; Saccharomyces cerevisiae; Saccharomyces cerevisiae Proteins
Язык текста: Английский
ISSN: 1388-1981
Lou W.
Ting H. -. H.-C.
Reynolds C. A.
Tyurina Y. Y.
Tyurin V. A.
Li Y.
Ji J.
Yu W.
Liang Z.
Stoyanovsky D. A.
Anthonymuthu T. S.
Frasso M. A.
Wipf P.
Greenberger J. S.
Bayır H.
Kagan V. E. Valerian E 1946-
Greenberg M. L.
Лоу W.
Тинг Х. -. Х.-C.
Реyнолдс C. А.
Тюрина Y. Y.
Тюрин В. А.
Ли Y.
Йи Й.
W.
Лианг З.
Стояновскy Д. А.
Антхонyмутху Т. С.
Фрассо М. А.
Wипф П.
Греенбергер Й. С.
Байıр Х.
Каган В. Е. Валериан Е 1946-
Греенберг М. Л.
Genetic re-engineering of polyunsaturated phospholipid profile of Saccharomyces cerevisiae identifies a novel role for Cld1 in mitigating the effects of cardiolipin peroxidation
Genetic re-engineering of polyunsaturated phospholipid profile of Saccharomyces cerevisiae identifies a novel role for Cld1 in mitigating the...
Текст визуальный непосредственный
Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids
Elsevier Science Publisher B.V.
Vol.1863, Issue10 P. 1354-1368
2018
Статья
cardiolipin Cld1 lipid peroxidation mass spectrometry (MS) polyunsaturated fatty acid (PUFA) remodeling yeast
cardiolipin cardiolipin d1 phospholipid polyunsaturated fatty acid unclassified drug acyl coenzyme A desaturase cardiolipin CLD1 protein, S cerevisiae delta-12 fatty acid desaturase phospholipase plant protein Saccharomyces cerevisiae protein unsaturated fatty acid apoptosis Article genetic engineering Hevea brasiliensis hydrolysis lipid peroxidation mitochondrial membrane potential nonhuman oxidative stress priority journal protein expression Saccharomyces cerevisiae signal transduction upregulation chemistry enzymology genetics growth, development and aging Hevea liquid chromatography mass spectrometry metabolism procedures Saccharomyces cerevisiae Cardiolipins Chromatography, Liquid Fatty Acid Desaturases Fatty Acids, Unsaturated Genetic Engineering Hevea Hydrolysis Lipid Peroxidation Mass Spectrometry Oxidative Stress Phospholipases Plant Proteins Saccharomyces cerevisiae Saccharomyces cerevisiae Proteins
Cardiolipin (CL) is a unique phospholipid localized almost exclusively within the mitochondrial membranes where it is synthesized. Newly synthesized CL undergoes acyl remodeling to produce CL species enriched with unsaturated acyl groups. Cld1 is the only identified CL-specific phospholipase in yeast and is required to initiate the CL remodeling pathway. In higher eukaryotes, peroxidation of CL, yielding CLOX, has been implicated in the cellular signaling events that initiate apoptosis. CLOX can undergo enzymatic hydrolysis, resulting in the release of lipid mediators with signaling properties. Our previous findings suggested that CLD1 expression is upregulated in response to oxidative stress, and that one of the physiological roles of CL remodeling is to remove peroxidized CL. To exploit the powerful yeast model to study functions of CLD1 in CL peroxidation, we expressed the H. brasiliensis Δ12-desaturase gene in yeast, which then synthesized poly unsaturated fatty acids(PUFAs) that a