The preparation of recombinant peptidoglycan-associated lipoprotein (pal) legionella pneumophila and characterisation of its immunogenic properties
Зенинскайа Н. А., Риабко А. К., Колесников А. В., Козyр А. В., Марин М. А., Фирстова В. В., Шемякин И. Г., Караулов А. В., Дятлов И. А.
Иммунология
Т. 39, Вып. 1, С. 50-55
Опубликовано: 2018
Тип ресурса: Статья
DOI:10.18821/0206-4952-2018-39-1-50-55
Аннотация:
A method for producing recombinant peptidoglycan-associated lipoprotein (PAL) L. pneumophila in a heterologous expression system consisting of chimerical protein His-SUMO-PAL with hexa-histidine tag (for chromatographic purification of the precursor protein) and the protein PAL, which separated by SUMO protease peptide was developed. The method allows to obtain recombinant PAL with a high degree of purity and a yield of 0.3 g per liter of bacterial culture without fermentation. The obtained recombinant PAL retains the native structure, immunogenic properties and it can be used for vaccine development and production of monoclonal antibodies as a diagnostic tool at Legionella infection. © 2018 Meditsina Publishers. All rights reserved.
Ключевые слова:
Legionella pneumophila; Legionellosis; Lymphocytes; PAL; Peptidoglycan-associated lipoprotein
lipoprotein; monoclonal antibody; peptidoglycan associated lipoprotein; polyhistidine tag; recombinant protein; unclassified drug; vaccine; antibody production; Article; bacterium culture; drug purity; drug structure; heterologous expression; Legionella pneumophila; legionnaire disease; nonhuman; vaccine immunogenicity
Язык текста: Русский
ISSN: 0206-4952
Зенинскайа Н. А.
Риабко А. К.
Колесников А. В.
Козyр А. В.
Марин М. А.
Фирстова В. В.
Шемякин И. Г.
Караулов А. В. Александр Викторович 1953-
Дятлов И. А.
Zeninskaya N. A.
Riabko A. K.
Kolesnikov A. V.
Kozyr A. V.
Marin M. A.
Firstova V. V.
Shemyakin I. G.
Karaulov A. V. Aleksandr Viktorovich 1953-
Dyatlov I. A.
The preparation of recombinant peptidoglycan-associated lipoprotein (pal) legionella pneumophila and characterisation of its immunogenic properties
Текст визуальный непосредственный
Иммунология
Издательство "Медицина"
Т. 39, Вып. 1 С. 50-55
2018
Статья
Legionella pneumophila Legionellosis Lymphocytes PAL Peptidoglycan-associated lipoprotein
lipoprotein monoclonal antibody peptidoglycan associated lipoprotein polyhistidine tag recombinant protein unclassified drug vaccine antibody production Article bacterium culture drug purity drug structure heterologous expression Legionella pneumophila legionnaire disease nonhuman vaccine immunogenicity
A method for producing recombinant peptidoglycan-associated lipoprotein (PAL) L. pneumophila in a heterologous expression system consisting of chimerical protein His-SUMO-PAL with hexa-histidine tag (for chromatographic purification of the precursor protein) and the protein PAL, which separated by SUMO protease peptide was developed. The method allows to obtain recombinant PAL with a high degree of purity and a yield of 0.3 g per liter of bacterial culture without fermentation. The obtained recombinant PAL retains the native structure, immunogenic properties and it can be used for vaccine development and production of monoclonal antibodies as a diagnostic tool at Legionella infection. © 2018 Meditsina Publishers. All rights reserved.