Isolation of Circulating Fetal Trophoblasts Using Inertial Microfluidics for Noninvasive Prenatal Testing

Winter M., Hardy T., Rezaei M., Nguyen V., Zander-Fox D., Ibragimi V. M. M., Thierry B.

Advanced Materials Technologies

Vol.3, Issue7, Num.1800066

Опубликовано: 2018

Тип ресурса: Статья

DOI:10.1002/admt.201800066

Аннотация:

While noninvasive prenatal testing based on cell-free fetal DNA has recently revolutionized the field of aneuploidy screening in pregnancy, it remains limited to aneuploidy and microdeletion screening, and is unable to reliably detect single gene disorders. A number of recent studies have demonstrated the potential of circulating trophoblastic cells in providing cell-based noninvasive diagnosis with sequencing or array-based assays. However, considering the extreme rarity of these cells in blood, efficient, high-throughput, and clinically applicable enrichment technologies are yet to be developed. This study demonstrates for the first time the utility of inertial microfluidics for efficient isolation of trophoblastic cells from maternal peripheral blood. Under optimal operating conditions, high-recovery yields (79[%]) are obtained using a trophoblastic cell-line, which is subsequently confirmed with analysis of maternal blood. Feasibility of obtaining a diagnosis from cells isolated from

Ключевые слова:

imaging flow cytometry; inertial microfluidics; noninvasive prenatal testing; trophoblastic cell isolation

Assays; Blood; Cell culture; Cells; Fluorescence microscopy; Microfluidics; Cell isolation; Fluorescence in situ hybridization; Inertial microfluidics; Maternal samples; Non-invasive diagnosis; Optimal operating conditions; Peripheral blood; Prenatal testing; Molecular biology

Язык текста: Английский

ISSN: 2365-709X

Winter M.

Hardy T.

Rezaei M.

Nguyen V.

Zander-Fox D.

Ibragimi V. M. M. Varkiani Madzhid Modzhtaba 1983-

Thierry B.

Wинтер М.

Хардy Т.

Резаеи М.

Нгуьен В.

Зандер-Фох Д.

Ибрагими В. М. М. Варкиани Маджид Моджтаба 1983-

Тхиеррy Б.

Isolation of Circulating Fetal Trophoblasts Using Inertial Microfluidics for Noninvasive Prenatal Testing

Текст визуальный непосредственный

Advanced Materials Technologies

Vol.3, Issue7 Num.1800066

2018

Статья

imaging flow cytometry inertial microfluidics noninvasive prenatal testing trophoblastic cell isolation

Assays Blood Cell culture Cells Fluorescence microscopy Microfluidics Cell isolation Fluorescence in situ hybridization Inertial microfluidics Maternal samples Non-invasive diagnosis Optimal operating conditions Peripheral blood Prenatal testing Molecular biology

While noninvasive prenatal testing based on cell-free fetal DNA has recently revolutionized the field of aneuploidy screening in pregnancy, it remains limited to aneuploidy and microdeletion screening, and is unable to reliably detect single gene disorders. A number of recent studies have demonstrated the potential of circulating trophoblastic cells in providing cell-based noninvasive diagnosis with sequencing or array-based assays. However, considering the extreme rarity of these cells in blood, efficient, high-throughput, and clinically applicable enrichment technologies are yet to be developed. This study demonstrates for the first time the utility of inertial microfluidics for efficient isolation of trophoblastic cells from maternal peripheral blood. Under optimal operating conditions, high-recovery yields (79[%]) are obtained using a trophoblastic cell-line, which is subsequently confirmed with analysis of maternal blood. Feasibility of obtaining a diagnosis from cells isolated from