Controlled gene and drug release from a liposomal delivery platform triggered by X-ray radiation
Deng W., Chen W., Clement S., Guller A. E., Zhao Z., Engel A., Goldys E. M.
Nature Communications
Vol.9, Issue1, Num.2713
Опубликовано: 2018
Тип ресурса: Статья
DOI:10.1038/s41467-018-05118-3
Аннотация:
Liposomes have been well established as an effective drug delivery system, due to simplicity of their preparation and unique characteristics. However conventional liposomes are unsuitable for the on-demand content release, which limits their therapeutic utility. Here we report X-ray-triggerable liposomes incorporating gold nanoparticles and photosensitizer verteporfin. The 6 MeV X-ray radiation induces verteporfin to produce singlet oxygen, which destabilises the liposomal membrane and causes the release of cargos from the liposomal cavity. This triggering strategy is demonstrated by the efficiency of gene silencing in vitro and increased effectiveness of chemotherapy in vivo. Our work indicates the feasibility of a combinatorial treatment and possible synergistic effects in the course of standard radiotherapy combined with chemotherapy delivered via X-ray-triggered liposomes. Importantly, our X-ray-mediated liposome release strategy offers prospects for deep tissue photodynamic therap
Ключевые слова:
antisense oligonucleotide; calcein; doxorubicin; gold nanoparticle; hypophysis adenylate cyclase activating polypeptide receptor; liposome; singlet oxygen; verteporfin; antineoplastic agent; antisense oligonucleotide; doxorubicin; etoposide; fluorescein derivative; folic acid; gold; hypophysis adenylate cyclase activating polypeptide receptor 1; metal nanoparticle; photosensitizing agent; verteporfin; chemotherapy; drug; gene; gene expression; gold; membrane; nanoparticle; X-ray; animal cell; animal experiment; animal model; animal tissue; Article; chemotherapy; colorectal cancer; controlled drug release; controlled study; cytotoxicity; drug efficacy; drug uptake; female; gene silencing; human; human cell; in vitro study; in vivo study; liposomal delivery; liposome membrane; mouse; nonhuman; PC12 cell line; photodynamic therapy; radiation dose; rat; X ray; animal; antagonists and inhibitors; chemistry; colon tumor; delayed release formulation; drug release; drug screening; gene silenci
Язык текста: Английский
ISSN: 2041-1723
Deng W.
Chen W.
Clement S.
Guller A. E. Anna Evgenyevna 1973-
Zhao Z.
Engel A.
Goldys E. M.
Денг W.
Чен W.
Cлемент С.
Гуллер А. Е. Анна Евгеньевна 1973-
Жао З.
Енгел А.
Голдyс Е. М.
Controlled gene and drug release from a liposomal delivery platform triggered by X-ray radiation
Текст визуальный непосредственный
Nature Communications
Nature Publishing Group
Vol.9, Issue1 Num.2713
2018
Статья
antisense oligonucleotide calcein doxorubicin gold nanoparticle hypophysis adenylate cyclase activating polypeptide receptor liposome singlet oxygen verteporfin antineoplastic agent antisense oligonucleotide doxorubicin etoposide fluorescein derivative folic acid gold hypophysis adenylate cyclase activating polypeptide receptor 1 metal nanoparticle photosensitizing agent verteporfin chemotherapy drug gene gene expression gold membrane nanoparticle X-ray animal cell animal experiment animal model animal tissue Article chemotherapy colorectal cancer controlled drug release controlled study cytotoxicity drug efficacy drug uptake female gene silencing human human cell in vitro study in vivo study liposomal delivery liposome membrane mouse nonhuman PC12 cell line photodynamic therapy radiation dose rat X ray animal antagonists and inhibitors chemistry colon tumor delayed release formulation drug release drug screening gene silenci
Liposomes have been well established as an effective drug delivery system, due to simplicity of their preparation and unique characteristics. However conventional liposomes are unsuitable for the on-demand content release, which limits their therapeutic utility. Here we report X-ray-triggerable liposomes incorporating gold nanoparticles and photosensitizer verteporfin. The 6 MeV X-ray radiation induces verteporfin to produce singlet oxygen, which destabilises the liposomal membrane and causes the release of cargos from the liposomal cavity. This triggering strategy is demonstrated by the efficiency of gene silencing in vitro and increased effectiveness of chemotherapy in vivo. Our work indicates the feasibility of a combinatorial treatment and possible synergistic effects in the course of standard radiotherapy combined with chemotherapy delivered via X-ray-triggered liposomes. Importantly, our X-ray-mediated liposome release strategy offers prospects for deep tissue photodynamic therap