Mechanisms of laser activation of chondrocytes in osteoarthritis healing
Alexandrovskaya Y. M., Baum O. I., Shekhter A. B., Petersen E. V., Tiflova O. A., Dmitriev A. K., Ulyanov V. A., Svistushkin V. M., Selezneva L. V., Sobol E. N.
Laser Physics Letters
Vol.15, Issue8, Num.085601
Опубликовано: 2018
Тип ресурса: Статья
DOI:10.1088/1612-202X/aac746
Аннотация:
Lasers offer new possibilities in the treatment of such widespread diseases as osteoarthritis, with both direct and indirect effects on cell metabolism. Cyclic hydrostatic pressure is one of the main natural stimuli of cartilage chondrocytes. The present work shows that hydrostatic stimulation with magnitudes of up to 20 MPa can be realized locally through infrared impact on the neighboring media of chondrocytes. We compare indirect (thermomechanical, λ = 1560 nm) and direct (photo-modulation, λ1 = 1560 nm, λ2 = 670 nm) laser effects on the synthetic activity of chondrocytes in cultures within a 1 min exposure time limit, to study separately the photo-modulation and thermomechanical components of laser impact. The chondrocyte activity was monitored by immunohistochemical analysis in normoxic and hypoxic conditions. Collagen II and proteoglycan accumulation increased significantly (up to 70[%]) after a pulsed thermomechanical laser impact. Thermomechanical laser irradiation showed the mor
Ключевые слова:
chondrocyte stimulation; infrared laser; photobiomodulation; thermomechanical effect
Язык текста: Английский
ISSN: 1612-202X
Alexandrovskaya Y. M.
Baum O. I.
Shekhter A. B. Anatolij Borukhovich 1933-
Petersen E. V. Elena Vladimirovna 1979-
Tiflova O. A.
Dmitriev A. K.
Ulyanov V. A.
Svistushkin V. M. Valerij Mikhaylovich 1960-
Selezneva L. V. Liliya Valeryevna 1988-
Sobol E. N.
Алехандровскайа Y. М.
Баум О. И.
Шехтер А. Б. Анатолий Борухович 1933-
Петерсен Е. В. Елена Владимировна 1979-
Тифлова О. А.
Дмитриев А. К.
Улянов В. А.
Свистушкин В. М. Валерий Михайлович 1960-
Селезнева Л. В. Лилия Валерьевна 1988-
Собол Е. Н.
Mechanisms of laser activation of chondrocytes in osteoarthritis healing
Текст визуальный непосредственный
Laser Physics Letters
Institute of Physics and IOP Publishing Limited
Vol.15, Issue8 Num.085601
2018
Статья
chondrocyte stimulation infrared laser photobiomodulation thermomechanical effect
Lasers offer new possibilities in the treatment of such widespread diseases as osteoarthritis, with both direct and indirect effects on cell metabolism. Cyclic hydrostatic pressure is one of the main natural stimuli of cartilage chondrocytes. The present work shows that hydrostatic stimulation with magnitudes of up to 20 MPa can be realized locally through infrared impact on the neighboring media of chondrocytes. We compare indirect (thermomechanical, λ = 1560 nm) and direct (photo-modulation, λ1 = 1560 nm, λ2 = 670 nm) laser effects on the synthetic activity of chondrocytes in cultures within a 1 min exposure time limit, to study separately the photo-modulation and thermomechanical components of laser impact. The chondrocyte activity was monitored by immunohistochemical analysis in normoxic and hypoxic conditions. Collagen II and proteoglycan accumulation increased significantly (up to 70[%]) after a pulsed thermomechanical laser impact. Thermomechanical laser irradiation showed the mor