Аннотация:
Background and aim: CDT is a collective biomarker including asialo- and disialo-Tf, but researchers have generally focused attention on disialo-Tf, because of its easier detectability, since asialo-Tf is typically not detectable by the current methods in abstinent individuals, social drinkers and in many alcohol abusers with moderate CDT increases. In the search of a confirmation marker of alcohol-related CDT increases, the detectability of asialo-Tf was re-evaluated comparatively by using CE vs. HPLC. Methods: 468 serum samples compulsorily drawn in a forensic/administrative context were analyzed by CE and HPLC to compare their sensitivity towards asialo-Tf. Results: CE allowed the identification of asialo-Tf in 108 out of 165 CDT “positive” cases, based on disialo-Tf measurement (cut-off 1.8[%]). HPLC showed a detectable asialo-Tf peak only in 2 cases. In addition, in some cases of disputed CDT increases, the quasi-absence of this Tf component in front of an important increase of disia
Ключевые слова:
Alcohol abuse; Asialo-Tf; Capillary electrophoresis; CDT; Forensic toxicology; HPLC
alcohol abuse; article; capillary electrophoresis; diagnosis; forensic toxicology; human; human tissue; major clinical study; alcoholism; analogs and derivatives; blood; capillary electrophoresis; high performance liquid chromatography; biological marker; carbohydrate-deficient transferrin; n acetylneuraminic acid; transferrin; Alcoholism; Biomarkers; Chromatography, High Pressure Liquid; Electrophoresis, Capillary; Humans; N-Acetylneuraminic Acid; Transferrin
Porpiglia N. M.
Savchuk S. A. Sergej Aleksandrovich 1958-
Appolonova S. A. Svetlana Aleksandrovna 1973-
Bortolotti F.
Tal`yaro F. Franko 1952-
Порпиглиа Н. М.
Савчук С. А. Сергей Александрович 1958-
Апполонова С. А. Светлана Александровна 1973-
Бортолотти Ф.
Тальяро Ф. Франко 1952-
Capillary Electrophoresis (CE) vs. HPLC in the determination of asialo-Tf, a crucial marker for the reliable interpretation of questioned CDT increases
Capillary Electrophoresis (CE) vs. HPLC in the determination of asialo-Tf, a crucial marker for the reliable interpretation of questioned CDT...
Текст визуальный непосредственный
Clinica Chimica Acta
Elsevier Science Publisher B.V.
Vol.486 P. 49-53
2018
Статья
Alcohol abuse Asialo-Tf Capillary electrophoresis CDT Forensic toxicology HPLC
alcohol abuse article capillary electrophoresis diagnosis forensic toxicology human human tissue major clinical study alcoholism analogs and derivatives blood capillary electrophoresis high performance liquid chromatography biological marker carbohydrate-deficient transferrin n acetylneuraminic acid transferrin Alcoholism Biomarkers Chromatography, High Pressure Liquid Electrophoresis, Capillary Humans N-Acetylneuraminic Acid Transferrin
Background and aim: CDT is a collective biomarker including asialo- and disialo-Tf, but researchers have generally focused attention on disialo-Tf, because of its easier detectability, since asialo-Tf is typically not detectable by the current methods in abstinent individuals, social drinkers and in many alcohol abusers with moderate CDT increases. In the search of a confirmation marker of alcohol-related CDT increases, the detectability of asialo-Tf was re-evaluated comparatively by using CE vs. HPLC. Methods: 468 serum samples compulsorily drawn in a forensic/administrative context were analyzed by CE and HPLC to compare their sensitivity towards asialo-Tf. Results: CE allowed the identification of asialo-Tf in 108 out of 165 CDT “positive” cases, based on disialo-Tf measurement (cut-off 1.8[%]). HPLC showed a detectable asialo-Tf peak only in 2 cases. In addition, in some cases of disputed CDT increases, the quasi-absence of this Tf component in front of an important increase of disia