Role of a receptor-like kinase K1 in pea Rhizobium symbiosis development
Kirienko A. N., Porozov Yu. B., Malkov N. V., Akhtemova G. A., Le S. C., Thompson R., Saffray C., Dalmais M., Bendahmane A., Tikhonovich I. A., Dolgikh E. A.
Planta
Vol.248, Issue5, P. 1101-1120
Опубликовано: 2018
Тип ресурса: Статья
DOI:10.1007/s00425-018-2944-4
Аннотация:
Main conclusion: The LysM receptor-like kinase K1 is involved in regulation of pea-rhizobial symbiosis development. The ability of the crop legume Pisum sativum L. to perceive the Nod factor rhizobial signals may depend on several receptors that differ in ligand structure specificity. Identification of pea mutants defective in two types of LysM receptor-like kinases (LysM-RLKs), SYM10 and SYM37, featuring different phenotypic manifestations and impaired at various stages of symbiosis development, corresponds well to this assumption. There is evidence that one of the receptor proteins involved in symbiosis initiation, SYM10, has an inactive kinase domain. This implies the presence of an additional component in the receptor complex, together with SYM10, that remains unknown. Here, we describe a new LysM-RLK, K1, which may serve as an additional component of the receptor complex in pea. To verify the function of K1 in symbiosis, several P. sativum non-nodulating mutants in the k1 gene wer
Ключевые слова:
Heterologous expression; Legume-Rhizobium symbiosis; LysM receptor-like kinases; Mutants; Nod factor perception; Pea; Pisum sativum L
plant protein; protein kinase; enzymology; genetic engineering; genetics; metabolism; microbiology; pea; physiology; plant leaf; procedures; reverse transcription polymerase chain reaction; Rhizobium leguminosarum; symbiosis; tobacco; transgenic plant; two hybrid system; Western blotting; Blotting, Western; Genetic Engineering; Peas; Plant Leaves; Plant Proteins; Plants, Genetically Modified; Protein Kinases; Reverse Transcriptase Polymerase Chain Reaction; Rhizobium leguminosarum; Symbiosis; Tobacco; Two-Hybrid System Techniques
Язык текста: Английский
ISSN: 1432-2048
Kirienko A. N.
Porozov Yu. B. Yurij Borisovich 1970-
Malkov N. V.
Akhtemova G. A.
Le S. C. Signor C.
Thompson R.
Saffray C.
Dalmais M.
Bendahmane A.
Tikhonovich I. A.
Dolgikh E. A.
Кириенко А. Н.
Порозов Ю. Б. Юрий Борисович 1970-
Малков Н. В.
Ахтемова Г. А.
Ле С. C. Сигнор C.
Тхомпсон Р.
Саффрай C.
Далмаис М.
Бендахмане А.
Тихонович И. А.
Долгих Е. А.
Role of a receptor-like kinase K1 in pea Rhizobium symbiosis development
Текст визуальный непосредственный
Planta
Springer-Verlag GmbH
Vol.248, Issue5 P. 1101-1120
2018
Статья
Heterologous expression Legume-Rhizobium symbiosis LysM receptor-like kinases Mutants Nod factor perception Pea Pisum sativum L
plant protein protein kinase enzymology genetic engineering genetics metabolism microbiology pea physiology plant leaf procedures reverse transcription polymerase chain reaction Rhizobium leguminosarum symbiosis tobacco transgenic plant two hybrid system Western blotting Blotting, Western Genetic Engineering Peas Plant Leaves Plant Proteins Plants, Genetically Modified Protein Kinases Reverse Transcriptase Polymerase Chain Reaction Rhizobium leguminosarum Symbiosis Tobacco Two-Hybrid System Techniques
Main conclusion: The LysM receptor-like kinase K1 is involved in regulation of pea-rhizobial symbiosis development. The ability of the crop legume Pisum sativum L. to perceive the Nod factor rhizobial signals may depend on several receptors that differ in ligand structure specificity. Identification of pea mutants defective in two types of LysM receptor-like kinases (LysM-RLKs), SYM10 and SYM37, featuring different phenotypic manifestations and impaired at various stages of symbiosis development, corresponds well to this assumption. There is evidence that one of the receptor proteins involved in symbiosis initiation, SYM10, has an inactive kinase domain. This implies the presence of an additional component in the receptor complex, together with SYM10, that remains unknown. Here, we describe a new LysM-RLK, K1, which may serve as an additional component of the receptor complex in pea. To verify the function of K1 in symbiosis, several P. sativum non-nodulating mutants in the k1 gene wer