Effect of Let-7b on the Differentiation of Bone Marrow Mesenchymal Stem Cells into Nerve Cells

Ли Й. -., Ли З., Ли Л., Жанг Й., Йиа Y. -., Ан Y.

Chinese General Practice

Т. 20, Вып. 33, С. 4156-4161

Опубликовано: 2017

Тип ресурса: Статья

DOI:10.3969/j.issn.1007-9572.2017.00.122

Аннотация:

Objective: To investigate the role of Let-7b in the differentiation of rat bone marrow mesenchymal stem cells (MSCs) into neurons.Methods: This study was conducted in 2013.Eighty SPF grade SD rats were randomly divided into blank control group, negative control group, Let-7b-up lentiviral vector (LV-rno-Let-7b-up) group, Let-7b-inhibition lentiviral vector (LV-rno-Let-7b-inhibition) group, 20 rats in each group.The negative control group, LV-rno-Let-7b-up group, LV-rno-Let-7b-inhibition group were injected EGFP blank virus respectively.Four groups of rats were sacrificed by cervical dislocation, and MSCs were obtained and cultured for 4 generations.Subculture of MSCs were infected with lentivirus, that is, the blank control group and the negative control group were not infected, LV-rno-Let-7b-up group, LV-rno-Let-7b-inhibition group were infected with LV-rno-Let-7b-up, Let-7b-inhibition, respectively.The survival rate of MSCs was detected by MTT before infection, at 24 h after infectio

Ключевые слова:

Cell differentiation; Lentivirus; Let-7b; Neurons; Stem cells, myeloid

Язык текста: Русский

ISSN: 1007-9572

Ли Й. -. Й.-Л.

Ли З.

Ли Л.

Жанг Й.

Йиа Y. -. Y.-Й.

Ан Y.

Li J. -. J.-L.

Li Z.

Li L.

Zhang J.

Jia Y. -. Y.-J.

An Y.

Effect of Let-7b on the Differentiation of Bone Marrow Mesenchymal Stem Cells into Nerve Cells

Текст визуальный непосредственный

Chinese General Practice

Т. 20, Вып. 33 С. 4156-4161

2017

Статья

Cell differentiation Lentivirus Let-7b Neurons Stem cells, myeloid

Objective: To investigate the role of Let-7b in the differentiation of rat bone marrow mesenchymal stem cells (MSCs) into neurons.Methods: This study was conducted in 2013.Eighty SPF grade SD rats were randomly divided into blank control group, negative control group, Let-7b-up lentiviral vector (LV-rno-Let-7b-up) group, Let-7b-inhibition lentiviral vector (LV-rno-Let-7b-inhibition) group, 20 rats in each group.The negative control group, LV-rno-Let-7b-up group, LV-rno-Let-7b-inhibition group were injected EGFP blank virus respectively.Four groups of rats were sacrificed by cervical dislocation, and MSCs were obtained and cultured for 4 generations.Subculture of MSCs were infected with lentivirus, that is, the blank control group and the negative control group were not infected, LV-rno-Let-7b-up group, LV-rno-Let-7b-inhibition group were infected with LV-rno-Let-7b-up, Let-7b-inhibition, respectively.The survival rate of MSCs was detected by MTT before infection, at 24 h after infectio