Cytoskeleton structure and total methylation of mouse cardiac and lung tissue during space flight
Ogneva I. V., Loktev S. S., Sychev V. N.
PLoS ONE
Vol.13, Issue5, Num.e0192643
Опубликовано: 2018
Тип ресурса: Статья
DOI:10.1371/journal.pone.0192643
Аннотация:
The purpose of this work was to evaluate the protein and mRNA expression levels of multiple cytoskeletal proteins in the cardiac and lung tissue of mice that were euthanized onboard the United States Orbital Segment of the International Space Station 37 days after the start of the SpaceX-4 mission (September 2014, USA). The results showed no changes in the cytoskeletal protein content in the cardiac and lung tissue of the mice, but there were significant changes in the mRNA expression levels of the associated genes, which may be due to an increase in total genome methylation. The mRNA expression levels of DNA methylases, the cytosine demethylases Tet1 and Tet3, histone acetylase and histone deacetylase did not change, and the mRNA expression level of cytosine demethylase Tet2 was significantly decreased. © 2018 Ogneva et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduct
Ключевые слова:
alpha actinin 1; alpha actinin 4; beta actin; cytoskeleton protein; desmin; DNA (cytosine 5) methyltransferase 1; DNA methyltransferase 3A; gamma actin; Hat1 protein; histone deacetylase 1; oxygenase; regulator protein; Tet1 protein; Tet3 protein; tubulin; unclassified drug; DNA binding protein; histone acetyltransferase; histone deacetylase; oncoprotein; TET1 protein, mouse; Tet2 protein, mouse; Tet3 protein, mouse; animal experiment; animal tissue; Article; controlled study; cytoskeleton; DNA methylation; heart tissue; lung parenchyma; mouse; mRNA expression level; nonhuman; protein content; protein expression; space flight; animal; C57BL mouse; cosmic radiation; DNA methylation; drug effect; gene expression regulation; genetics; heart; lung; metabolism; physiology; radiation response; Animals; Cosmic Radiation; DNA Methylation; DNA-Binding Proteins; Gene Expression Regulation; Heart; Histone Acetyltransferases; Histone Deacetylases; Lung; Mice; Mice, Inbred C57BL; Proto-Oncogene Pro
Язык текста: Английский
ISSN: 1932-6203
Ogneva I. V. Irina Vladimirovna 1979-
Loktev S. S.
Sychev V. N.
Огнева И. В. Ирина Владимировна 1979-
Локтев С. С.
Сyчев В. Н.
Cytoskeleton structure and total methylation of mouse cardiac and lung tissue during space flight
Текст визуальный непосредственный
PLoS ONE
Vol.13, Issue5 Num.e0192643
2018
Статья
alpha actinin 1 alpha actinin 4 beta actin cytoskeleton protein desmin DNA (cytosine 5) methyltransferase 1 DNA methyltransferase 3A gamma actin Hat1 protein histone deacetylase 1 oxygenase regulator protein Tet1 protein Tet3 protein tubulin unclassified drug DNA binding protein histone acetyltransferase histone deacetylase oncoprotein TET1 protein, mouse Tet2 protein, mouse Tet3 protein, mouse animal experiment animal tissue Article controlled study cytoskeleton DNA methylation heart tissue lung parenchyma mouse mRNA expression level nonhuman protein content protein expression space flight animal C57BL mouse cosmic radiation DNA methylation drug effect gene expression regulation genetics heart lung metabolism physiology radiation response Animals Cosmic Radiation DNA Methylation DNA-Binding Proteins Gene Expression Regulation Heart Histone Acetyltransferases Histone Deacetylases Lung Mice Mice, Inbred C57BL Proto-Oncogene Pro
The purpose of this work was to evaluate the protein and mRNA expression levels of multiple cytoskeletal proteins in the cardiac and lung tissue of mice that were euthanized onboard the United States Orbital Segment of the International Space Station 37 days after the start of the SpaceX-4 mission (September 2014, USA). The results showed no changes in the cytoskeletal protein content in the cardiac and lung tissue of the mice, but there were significant changes in the mRNA expression levels of the associated genes, which may be due to an increase in total genome methylation. The mRNA expression levels of DNA methylases, the cytosine demethylases Tet1 and Tet3, histone acetylase and histone deacetylase did not change, and the mRNA expression level of cytosine demethylase Tet2 was significantly decreased. © 2018 Ogneva et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduct