Expression of genes for neurotransmitter transporters in astrocytes in different brain regions in experiment
Шушарина Н. Н., Патрушев М. В., Силина Е. В., Стюпин В. А., Литвицкий П. Ф., Орлова А. С.
Журнал неврологии и психиатрии им. C.C. Корсакова
Т. 118, Вып. 6, С. 58-64
Опубликовано: 2018
Тип ресурса: Статья
DOI:10.17116/jnevro20181186158
Аннотация:
Objective. To investigate the expression of transporters of different neurotransmitters (glutamate, aspartate, lactate, choline) in the culture of astrocytes isolated from different regions of the brain (cortex, hippocampus and brainstem) in 3- and 11-day rats. Material and methods. An experimental study was performed on 24 3-(n=12) and 11-days (n=12) old rats (Rattus norvegicus). The results of high-performance sequencing were analyzed. Results. The expression of glutamate and aspartate transporters in the brainstem of 3-day rats was higher than in other regions, however, an opposite effect was observed in 11-day rats. The expression of lactate transporters with age became identical to those of the cortex. Conclusion. The data demonstrate the particular qualities of neuro-astrocytic connections and the important role of astrocytes in signal transmission. Results of the study performed by using genetic methods developed by the authors for the study of neurotransmitter transporters make
Ключевые слова:
Aspartate; Astrocytes; Choline; Gene expression; Genetic methods of neurogenesis registration; Glutamate; Lactate; Neuroglia; Neurotransmitters; Transporters
glutamic acid; neurotransmitter transporter; neurotransmitter transporter; animal experiment; Article; astrocyte; brain region; brain stem; controlled study; degenerative disease; gene expression; nervous system development; nonhuman; signal transduction; animal; brain; hippocampus; rat; Animals; Astrocytes; Brain; Glutamic Acid; Hippocampus; Neurotransmitter Transport Proteins; Rats
Язык текста: Русский
ISSN: 2309-4729
Шушарина Н. Н.
Патрушев М. В.
Силина Е. В. Екатерина Владимировна 1980-
Стюпин В. А.
Литвицкий П. Ф. Петр Францевич 1947-
Орлова А. С. Александра Сергеевна 1986-
Shusharina N. N.
Patrushev M. V.
Silina E. V. Ekaterina Vladimirovna 1980-
Stupin V. A.
Litvitskij P. F. Petr Frantsevich 1947-
Orlova A. S. Aleksandra Sergeevna 1986-
Expression of genes for neurotransmitter transporters in astrocytes in different brain regions in experiment
Текст визуальный непосредственный
Журнал неврологии и психиатрии им. C.C. Корсакова
Издательство Медиа Сфера
Т. 118, Вып. 6 С. 58-64
2018
Статья
Aspartate Astrocytes Choline Gene expression Genetic methods of neurogenesis registration Glutamate Lactate Neuroglia Neurotransmitters Transporters
glutamic acid neurotransmitter transporter neurotransmitter transporter animal experiment Article astrocyte brain region brain stem controlled study degenerative disease gene expression nervous system development nonhuman signal transduction animal brain hippocampus rat Animals Astrocytes Brain Glutamic Acid Hippocampus Neurotransmitter Transport Proteins Rats
Objective. To investigate the expression of transporters of different neurotransmitters (glutamate, aspartate, lactate, choline) in the culture of astrocytes isolated from different regions of the brain (cortex, hippocampus and brainstem) in 3- and 11-day rats. Material and methods. An experimental study was performed on 24 3-(n=12) and 11-days (n=12) old rats (Rattus norvegicus). The results of high-performance sequencing were analyzed. Results. The expression of glutamate and aspartate transporters in the brainstem of 3-day rats was higher than in other regions, however, an opposite effect was observed in 11-day rats. The expression of lactate transporters with age became identical to those of the cortex. Conclusion. The data demonstrate the particular qualities of neuro-astrocytic connections and the important role of astrocytes in signal transmission. Results of the study performed by using genetic methods developed by the authors for the study of neurotransmitter transporters make