Quantitative Prediction of Yield in Transglycosylation Reaction Catalyzed by Nucleoside Phosphorylases
Alexeev C. S., Kulikova I. V., Gavryushov S. A., Tararov V. I., Mikhailov S. N.
ADVANCED SYNTHESIS & CATALYSIS
Vol.360, Issue16, P. 3090-3096
Опубликовано: 2018
Тип ресурса: Статья
DOI:10.1002/adsc.201800411
Аннотация:
Phosphorolytic transglycosylation catalyzed by nucleoside phosphorylases is an important biotechnological process. The reaction is reversible, and the yield of the target nucleoside depends on its concentration at the equilibrium state. We have shown that initial concentrations of the starting compounds and the phosphorolysis equilibrium constants of starting and final glycosides determine concentrations of all the components at the equilibrium state. Based on that, we developed a novel quantitative approach for the prediction of yields in transglycosylation reactions. This method simplifies the choice of reagent concentrations and their ratios for the maximization of the target nucleoside yield. It is advantageous over widely applied blind and cumbersome trial-and-error approach and can reduce the required chemical and energy resources. The described algorithm could also be applied for other equilibrium transfer reactions. (Figure presented.). © 2018 Wiley-VCH Verlag GmbH & Co. KGaA,
Ключевые слова:
equilibrium constants; nucleoside phosphorolysis; nucleoside phosphorylases; prediction and maximization of reaction outcome; transglycosylation
Язык текста: Английский
ISSN: 1615-4169
Alexeev C. S.
Kulikova I. V.
Gavryushov S. A. Sergej Aleksandrovich 1964-
Tararov V. I.
Mikhailov S. N.
Алехеев C. С.
Куликова И. В.
Гаврюшов С. А. Сергей Александрович 1964-
Тараров В. И.
Михаилов С. Н.
Quantitative Prediction of Yield in Transglycosylation Reaction Catalyzed by Nucleoside Phosphorylases
Текст визуальный непосредственный
ADVANCED SYNTHESIS & CATALYSIS
Wiley Interscience
Vol.360, Issue16 P. 3090-3096
2018
Статья
equilibrium constants nucleoside phosphorolysis nucleoside phosphorylases prediction and maximization of reaction outcome transglycosylation
Phosphorolytic transglycosylation catalyzed by nucleoside phosphorylases is an important biotechnological process. The reaction is reversible, and the yield of the target nucleoside depends on its concentration at the equilibrium state. We have shown that initial concentrations of the starting compounds and the phosphorolysis equilibrium constants of starting and final glycosides determine concentrations of all the components at the equilibrium state. Based on that, we developed a novel quantitative approach for the prediction of yields in transglycosylation reactions. This method simplifies the choice of reagent concentrations and their ratios for the maximization of the target nucleoside yield. It is advantageous over widely applied blind and cumbersome trial-and-error approach and can reduce the required chemical and energy resources. The described algorithm could also be applied for other equilibrium transfer reactions. (Figure presented.). © 2018 Wiley-VCH Verlag GmbH & Co. KGaA,