Static droplet array for culturing single live adherent cells in an isolated chemical microenvironment
Hassanzadeh-Barforoushi A., Law A. M. K., Hejri A., Asadnia M., Ormandy C. J., Gallego-Ortega D., Ibragimi V. M. M.
Lab on a Chip: Miniaturisation for Chemistry and Biology
Vol.18, Issue15, P. 2156-2166
Опубликовано: 2018
Тип ресурса: Статья
Аннотация:
We present here a new method to easily and reliably generate an array of hundreds of dispersed nanoliter-volume semi-droplets for single-cells culture and analysis. The liquid segmentation step occurs directly in indexed traps by a tweezer-like mechanism and is stabilized by spatial confinement. Unlike common droplet-based techniques, the semi-droplet wets its surrounding trap walls thus supporting the culturing of both adherent and non-adherent cells. To eliminate cross-droplet cell migration and chemical cross-talk each semi-droplet is separated from a nearby trap by an ∼80 pL air plug. The overall setup and injection procedure takes less than 10 minutes, is insensitive to fabrication defects and supports cell recovery for downstream analysis. The method offers a new approach to easily capture, image and culture single cells in a chemically isolated microenvironment as a preliminary step towards high-throughput single-cell assays. © 2018 The Royal Society of Chemistry.
Ключевые слова:
adherent cell; article; cell assay; cell migration; human cell; injection; microenvironment; cell adhesion; cell culture technique; cell survival; devices; equipment design; human; lab on a chip; single cell analysis; tumor cell line; tumor microenvironment; Cell Adhesion; Cell Culture Techniques; Cell Line, Tumor; Cell Survival; Cellular Microenvironment; Equipment Design; Humans; Lab-On-A-Chip Devices; Single-Cell Analysis
Язык текста: Английский
ISSN: 1473-0189
Hassanzadeh-Barforoushi A.
Law A. M. K.
Hejri A.
Asadnia M.
Ormandy C. J.
Gallego-Ortega D.
Ibragimi V. M. M. Varkiani Madzhid Modzhtaba 1983-
Хассанзадех-Барфороуши А.
Лаw А. М. К.
Хейри А.
Асадниа М.
Ормандy C. Й.
Галлего-Ортега Д.
Ибрагими В. М. М. Варкиани Маджид Моджтаба 1983-
Static droplet array for culturing single live adherent cells in an isolated chemical microenvironment
Текст визуальный непосредственный
Lab on a Chip: Miniaturisation for Chemistry and Biology
Royal Society of Chemistry
Vol.18, Issue15 P. 2156-2166
2018
Статья
adherent cell article cell assay cell migration human cell injection microenvironment cell adhesion cell culture technique cell survival devices equipment design human lab on a chip single cell analysis tumor cell line tumor microenvironment Cell Adhesion Cell Culture Techniques Cell Line, Tumor Cell Survival Cellular Microenvironment Equipment Design Humans Lab-On-A-Chip Devices Single-Cell Analysis
We present here a new method to easily and reliably generate an array of hundreds of dispersed nanoliter-volume semi-droplets for single-cells culture and analysis. The liquid segmentation step occurs directly in indexed traps by a tweezer-like mechanism and is stabilized by spatial confinement. Unlike common droplet-based techniques, the semi-droplet wets its surrounding trap walls thus supporting the culturing of both adherent and non-adherent cells. To eliminate cross-droplet cell migration and chemical cross-talk each semi-droplet is separated from a nearby trap by an ∼80 pL air plug. The overall setup and injection procedure takes less than 10 minutes, is insensitive to fabrication defects and supports cell recovery for downstream analysis. The method offers a new approach to easily capture, image and culture single cells in a chemically isolated microenvironment as a preliminary step towards high-throughput single-cell assays. © 2018 The Royal Society of Chemistry.