Cytokinin perception in potato: New features of canonical players
Lomin S. N., Myakushina Y. A., Kolachevskaya O. O., Getman I. A., Arkhipov D. V., Savelieva E. M., Osolodkin D. I., Romanov G. A.
Journal of Experimental Botany
Vol.69, Issue16, P. 3839-3853
Опубликовано: 2018
Тип ресурса: Статья
Аннотация:
Potato is the most economically important non-cereal food crop. Tuber formation in potato is regulated by phytohormones, cytokinins (CKs) in particular. The present work studied CK signal perception in potato. The sequenced potato genome of doubled monoploid Phureja was used for bioinformatic analysis and as a tool for identification of putative CK receptors from autotetraploid potato cv. Désirée. All basic elements of multistep phosphorelay required for CK signal transduction were identified in the Phureja genome, including three genes orthologous to three CK receptor genes (AHK 2-4) of Arabidopsis. As distinct from Phureja, autotetraploid potato contains at least two allelic isoforms of each receptor type. Putative receptor genes from Désirée plants were cloned, sequenced and expressed, and the main characteristics of encoded proteins were determined, in particular their consensus motifs, modelled structure, ligand-binding properties, and ability to transmit CK signals. In all studie
Ключевые слова:
CHASE domain-containing histidine kinase; cytokinin; cytokinin receptor; cytokinin signaling; gene expression; hormone perception; potato; Solanum tuberosum
cell surface receptor; cytokinin; sucrose; allele; amino acid sequence; biotechnology; chemistry; genetics; homozygote; metabolism; phylogeny; plant gene; potato; promoter region; sequence homology; signal transduction; Alleles; Amino Acid Sequence; Biotechnology; Cytokinins; Genes, Plant; Homozygote; Phylogeny; Promoter Regions, Genetic; Receptors, Cell Surface; Sequence Homology, Amino Acid; Signal Transduction; Solanum tuberosum; Sucrose
Язык текста: Английский
ISSN: 1460-2431
Lomin S. N.
Myakushina Y. A.
Kolachevskaya O. O.
Getman I. A.
Arkhipov D. V.
Savelieva E. M.
Osolodkin D. I. Dmitrij Ivanovich 1985-
Romanov G. A.
Ломин С. Н.
Мякушина Y. А.
Колачевскайа О. О.
Гетман И. А.
Архипов Д. В.
Савелиева Е. М.
Осолодкин Д. И. Дмитрий Иванович 1985-
Романов Г. А.
Cytokinin perception in potato: New features of canonical players
Текст визуальный непосредственный
Journal of Experimental Botany
Oxford University Press
Vol.69, Issue16 P. 3839-3853
2018
Статья
CHASE domain-containing histidine kinase cytokinin cytokinin receptor cytokinin signaling gene expression hormone perception potato Solanum tuberosum
cell surface receptor cytokinin sucrose allele amino acid sequence biotechnology chemistry genetics homozygote metabolism phylogeny plant gene potato promoter region sequence homology signal transduction Alleles Amino Acid Sequence Biotechnology Cytokinins Genes, Plant Homozygote Phylogeny Promoter Regions, Genetic Receptors, Cell Surface Sequence Homology, Amino Acid Signal Transduction Solanum tuberosum Sucrose
Potato is the most economically important non-cereal food crop. Tuber formation in potato is regulated by phytohormones, cytokinins (CKs) in particular. The present work studied CK signal perception in potato. The sequenced potato genome of doubled monoploid Phureja was used for bioinformatic analysis and as a tool for identification of putative CK receptors from autotetraploid potato cv. Désirée. All basic elements of multistep phosphorelay required for CK signal transduction were identified in the Phureja genome, including three genes orthologous to three CK receptor genes (AHK 2-4) of Arabidopsis. As distinct from Phureja, autotetraploid potato contains at least two allelic isoforms of each receptor type. Putative receptor genes from Désirée plants were cloned, sequenced and expressed, and the main characteristics of encoded proteins were determined, in particular their consensus motifs, modelled structure, ligand-binding properties, and ability to transmit CK signals. In all studie