Molecular-Genetic Characterization of Human Rotavirus A Strains Circulating in Moscow, Russia (2009–2014)
Kiseleva V., Faizuloev E., Meskina E., Marova A., Oksanich A., Samartseva T., Bakhtoyarov G., Bochkareva N., Filatov N. N., Linok A., Ammour Y., Zverev V.
Virologica Sinica
Vol.33, Issue4, P. 304-313
Опубликовано: 2018
Тип ресурса: Статья
DOI:10.1007/s12250-018-0043-0
Аннотация:
Enteric viruses are the most common cause of acute gastroenteritis (AGE) in young children and a significant public health problem globally. Hospital admissions of children under 5 years of age with diarrhea are primarily associated with group A rotavirus (RVA) infection. In this retrospective study, the population structure of viruses linked to AGE etiology in young children hospitalized with AGE in Moscow was evaluated, and molecular characterization of RVA strains was performed. Fecal specimens were collected from children under 5 years old hospitalized with AGE between 2009 and 2014 in Moscow, Russia. Multiplex real-time reverse transcription PCR was used to detect enteric viruses and for G/[P]-genotyping of isolated RVAs. Sequencing of RVA VP7 and VP4 cDNA fragments was used to validate the data obtained by PCR-genotyping. The main causes for hospitalization of children with AGE were RVA (40.1[%]), followed by noroviruses (11.4[%]), while adenoviruses, astroviruses, sapoviruses, enter
Ключевые слова:
Acute gastroenteritis (AGE); Moscow; Rotaviral enteritis; Rotavirus G/[P] genotype; Rotavirus vaccine
complementary DNA; capsid protein; virus antigen; virus DNA; VP7 protein, Rotavirus; acute gastroenteritis; Adenoviridae; Article; Astroviridae; child; enteric virus; Enterovirus; epidemiological monitoring; genotype; hospital infection; hospitalization; human; major clinical study; Norovirus; Orthoreovirus; population structure; prevalence; real time polymerase chain reaction; retrospective study; reverse transcription polymerase chain reaction; Rotavirus A; Russian Federation; summer; virus etiology; virus infection; classification; cross infection; DNA sequence; feces; gastroenteritis; genetic variation; genetics; infant; newborn; phylogeny; preschool child; Rotavirus; Rotavirus infection; virology; Antigens, Viral; Capsid Proteins; Child, Preschool; Cross Infection; DNA, Viral; Feces; Gastroenteritis; Genetic Variation; Genotype; Humans; Infant; Infant, Newborn; Moscow; Phylogeny; Prevalence; Retrospective Studies; Rotavirus; Rotavirus Infections; Sequence Analysis, DNA
Язык текста: Английский
ISSN: 1995-820X
Kiseleva V.
Faizuloev E.
Meskina E.
Marova A.
Oksanich A.
Samartseva T.
Bakhtoyarov G.
Bochkareva N.
Filatov N. N. Nikolay Nikolaevich 1954-
Linok A.
Ammour Y.
Zverev V.
Киселева В.
Фаизулоев Е.
Мескина Е.
Марова А.
Оксанич А.
Самарцева Т.
Бахтояров Г.
Бочкарева Н.
Филатов Н. Н. Николай Николаевич 1954-
Линок А.
Аммоур Y.
Зверев В.
Molecular-Genetic Characterization of Human Rotavirus A Strains Circulating in Moscow, Russia (2009–2014)
Текст визуальный непосредственный
Virologica Sinica
Vol.33, Issue4 P. 304-313
2018
Статья
Acute gastroenteritis (AGE) Moscow Rotaviral enteritis Rotavirus G/[P] genotype Rotavirus vaccine
complementary DNA capsid protein virus antigen virus DNA VP7 protein, Rotavirus acute gastroenteritis Adenoviridae Article Astroviridae child enteric virus Enterovirus epidemiological monitoring genotype hospital infection hospitalization human major clinical study Norovirus Orthoreovirus population structure prevalence real time polymerase chain reaction retrospective study reverse transcription polymerase chain reaction Rotavirus A Russian Federation summer virus etiology virus infection classification cross infection DNA sequence feces gastroenteritis genetic variation genetics infant newborn phylogeny preschool child Rotavirus Rotavirus infection virology Antigens, Viral Capsid Proteins Child, Preschool Cross Infection DNA, Viral Feces Gastroenteritis Genetic Variation Genotype Humans Infant Infant, Newborn Moscow Phylogeny Prevalence Retrospective Studies Rotavirus Rotavirus Infections Sequence Analysis, DNA
Enteric viruses are the most common cause of acute gastroenteritis (AGE) in young children and a significant public health problem globally. Hospital admissions of children under 5 years of age with diarrhea are primarily associated with group A rotavirus (RVA) infection. In this retrospective study, the population structure of viruses linked to AGE etiology in young children hospitalized with AGE in Moscow was evaluated, and molecular characterization of RVA strains was performed. Fecal specimens were collected from children under 5 years old hospitalized with AGE between 2009 and 2014 in Moscow, Russia. Multiplex real-time reverse transcription PCR was used to detect enteric viruses and for G/[P]-genotyping of isolated RVAs. Sequencing of RVA VP7 and VP4 cDNA fragments was used to validate the data obtained by PCR-genotyping. The main causes for hospitalization of children with AGE were RVA (40.1[%]), followed by noroviruses (11.4[%]), while adenoviruses, astroviruses, sapoviruses, enter