Paip2 is localized to active promoters and loaded onto nascent mRNA in Drosophila
Kachaev Z. M., Lebedeva L. A., Kozlov E. N., Toropygin I. Y., Schedl P., Shidlovskij Yu. V.
Cell Cycle
Vol.17, Issue14, P. 1708-1720
Опубликовано: 2018
Тип ресурса: Статья
DOI:10.1080/15384101.2018.1496738
Аннотация:
Paip2 (Poly(A)-binding protein–interacting protein 2) is a conserved metazoan-specific protein that has been implicated in regulating the translation and stability of mRNAs. However, we have found that Paip2 is not restricted to the cytoplasm but is also found in the nucleus in Drosophila embryos, salivary glands, testes, and tissue culture cells. Nuclear Paip2 is associated with chromatin, and in chromatin immunoprecipitation experiments it maps to the promoter regions of active genes. However, this chromatin association is indirect, as it is RNA-dependent. Thus, Paip2 is one more item in the growing list of translation factors that are recruited to mRNAs co-transcriptionally. © 2018, © 2018 Informa UK Limited, trading as Taylor & Francis Group.
Ключевые слова:
mRNA; nucleus; Paip2; promoter; transcription
messenger RNA; polyadenylic acid binding protein; polyadenylic acid binding protein interacting protein 2; unclassified drug; carrier protein; Drosophila protein; messenger RNA; Paip2 protein, Drosophila; protein binding; Article; cell nucleus; chromatin; controlled study; cytoplasm; Drosophila; nonhuman; promoter region; protein binding; protein localization; RNA transcription; animal; cell line; Drosophila melanogaster; genetics; male; metabolism; nonmammalian embryo; testis; Animals; Carrier Proteins; Cell Line; Chromatin; Drosophila melanogaster; Drosophila Proteins; Embryo, Nonmammalian; Male; Promoter Regions, Genetic; Protein Binding; RNA, Messenger; Testis
Язык текста: Английский
ISSN: 1551-4005
Kachaev Z. M.
Lebedeva L. A.
Kozlov E. N.
Toropygin I. Y.
Schedl P.
Shidlovskij Yu. V. Yulij Valeryevich 1979-
Качаев З. М.
Лебедева Л. А.
Козлов Е. Н.
Торопyгин И. Y.
Счедл П.
Шидловский Ю. В. Юлий Валерьевич 1979-
Paip2 is localized to active promoters and loaded onto nascent mRNA in Drosophila
Текст визуальный непосредственный
Cell Cycle
Landes Bioscience
Vol.17, Issue14 P. 1708-1720
2018
Статья
mRNA nucleus Paip2 promoter transcription
messenger RNA polyadenylic acid binding protein polyadenylic acid binding protein interacting protein 2 unclassified drug carrier protein Drosophila protein messenger RNA Paip2 protein, Drosophila protein binding Article cell nucleus chromatin controlled study cytoplasm Drosophila nonhuman promoter region protein binding protein localization RNA transcription animal cell line Drosophila melanogaster genetics male metabolism nonmammalian embryo testis Animals Carrier Proteins Cell Line Chromatin Drosophila melanogaster Drosophila Proteins Embryo, Nonmammalian Male Promoter Regions, Genetic Protein Binding RNA, Messenger Testis
Paip2 (Poly(A)-binding protein–interacting protein 2) is a conserved metazoan-specific protein that has been implicated in regulating the translation and stability of mRNAs. However, we have found that Paip2 is not restricted to the cytoplasm but is also found in the nucleus in Drosophila embryos, salivary glands, testes, and tissue culture cells. Nuclear Paip2 is associated with chromatin, and in chromatin immunoprecipitation experiments it maps to the promoter regions of active genes. However, this chromatin association is indirect, as it is RNA-dependent. Thus, Paip2 is one more item in the growing list of translation factors that are recruited to mRNAs co-transcriptionally. © 2018, © 2018 Informa UK Limited, trading as Taylor & Francis Group.