Diversity and evolution of anuran trypanosomes: Insights from the study of European species
Spodareva V. V., Grybchuk-Ieremenko A., Losev A., Votýpka J., Lukeš J., Yurchenko V., Kostygov A. Y.
Parasites & Vectors
Vol.11, Issue1, Num.447
Опубликовано: 2018
Тип ресурса: Статья
DOI:10.1186/s13071-018-3023-1
Аннотация:
Background: Amphibian trypanosomes were the first ever described trypanosomatids. Nevertheless, their taxonomy remains entangled because of pleomorphism and high prevalence of mixed infections. Despite the fact that the first species in this group were described in Europe, virtually none of the trypanosomes from European anurans was analyzed using modern molecular methods. Methods: In this study, we explored the diversity and phylogeny of trypanosomes in true frogs from Europe using light microscopy and molecular methods. Results: A comparison of observed morphotypes with previous descriptions allowed us to reliably identify three Trypanosoma spp., whereas the remaining two strains were considered to represent novel taxa. In all cases, more than one morphotype per blood sample was observed, indicating mixed infections. One hundred and thirty obtained 18S rRNA gene sequences were unambiguously subdivided into five groups, correspondent to the previously recognized or novel taxa of anura
Ключевые слова:
Evolution; Frog trypanosomes; Mixed infections; Trypanosomatidae
Anura; Article; blood sampling; genetic analysis; geographic distribution; microscopy; mixed infection; molecular genetics; morphological trait; morphotype; nonhuman; nucleotide sequence; phylogeny; RNA sequence; species diversity; species identification; taxonomic identification; Trypanosoma; trypanosomiasis; animal; blood; Czechoslovakia; genetic variation; genetics; molecular cloning; parasitology; physiology; polymerase chain reaction; species difference; Trypanosoma; Ukraine; protozoal RNA; RNA 18S; Animals; Anura; Cloning, Molecular; Czechoslovakia; Genetic Variation; Phylogeny; Polymerase Chain Reaction; RNA, Protozoan; RNA, Ribosomal, 18S; Species Specificity; Trypanosoma; Ukraine
Язык текста: Английский
ISSN: 1756-3305
Spodareva V. V.
Grybchuk-Ieremenko A.
Losev A.
Votýpka J.
Lukeš J.
Yurchenko V. Vyacheslav 1972-
Kostygov A. Y.
Сподарева В. В.
Грyбчук-Иеременко А.
Лосев А.
Вотýпка Й.
Лукеš Й.
Юрченко В. Вячеслав 1972-
Костyгов А. Y.
Diversity and evolution of anuran trypanosomes: Insights from the study of European species
Текст визуальный непосредственный
Parasites & Vectors
BioMed Central Ltd.
Vol.11, Issue1 Num.447
2018
Статья
Evolution Frog trypanosomes Mixed infections Trypanosomatidae
Anura Article blood sampling genetic analysis geographic distribution microscopy mixed infection molecular genetics morphological trait morphotype nonhuman nucleotide sequence phylogeny RNA sequence species diversity species identification taxonomic identification Trypanosoma trypanosomiasis animal blood Czechoslovakia genetic variation genetics molecular cloning parasitology physiology polymerase chain reaction species difference Trypanosoma Ukraine protozoal RNA RNA 18S Animals Anura Cloning, Molecular Czechoslovakia Genetic Variation Phylogeny Polymerase Chain Reaction RNA, Protozoan RNA, Ribosomal, 18S Species Specificity Trypanosoma Ukraine
Background: Amphibian trypanosomes were the first ever described trypanosomatids. Nevertheless, their taxonomy remains entangled because of pleomorphism and high prevalence of mixed infections. Despite the fact that the first species in this group were described in Europe, virtually none of the trypanosomes from European anurans was analyzed using modern molecular methods. Methods: In this study, we explored the diversity and phylogeny of trypanosomes in true frogs from Europe using light microscopy and molecular methods. Results: A comparison of observed morphotypes with previous descriptions allowed us to reliably identify three Trypanosoma spp., whereas the remaining two strains were considered to represent novel taxa. In all cases, more than one morphotype per blood sample was observed, indicating mixed infections. One hundred and thirty obtained 18S rRNA gene sequences were unambiguously subdivided into five groups, correspondent to the previously recognized or novel taxa of anura