p62/SQSTM1 expression in canine mammary tumours: Evolutionary notes
Mariotti F., Magi G. E., Gavazza A., Vincenzetti S., Komissarov A., Shnejder A. M., Venantsi F.
Veterinary and Comparative Oncology
Vol.17, Issue4, P. 570-577
Опубликовано: 2019
Тип ресурса: Статья
Аннотация:
Recent studies highlighted the role of autophagy as a cardinal regulatory system for homeostasis and cancer-related signalling pathways. In this context, the deregulated expression of p62 – Sequestosome1 (p62/SQSTM1) – a protein acting both as an autophagy receptor and signalling hub, has been associated with tumour development and chronic inflammation. Multiple clinical studies test drugs targeting autophagy, and even more research is on the way to clinical trials. However, no comparative investigations have been carried out to identify adequate preclinical models to assess p62-based medicine. In veterinary oncology the role of p62 in cancer-related pathways has been largely ignored. We compared p62 sequences in multiple organisms and found that canine p62 significantly diverges from the humans and from other animals sequences. Then, we chart by immunohistochemistry the expression levels of p62 in canine mammary tumours. A total of 66 tumours and 10 non-neoplastic mammary samples were
Ключевые слова:
autophagy; dog; gene homology; immunohistochemistry; mammary tumours; p62/SQSTM1
isoprotein; recombinant protein; sequestosome 1; antineoplastic agent; sequestosome 1; adenoma; amino acid sequence; animal tissue; Article; autophagy; bitch; breast tissue; breast tumor; cancer cell; cancer grading; Canis; chronic inflammation; controlled study; dog breed; epithelium cell; epitope mapping; histology; hyperplasia; immunohistochemistry; immunoreactivity; intraductal papillary mucinous tumor; morphology; multiple sequence alignment; nonhuman; papillary carcinoma; phylogeny; protein expression; protein expression level; scoring system; sequence alignment; sequence homology; signal transduction; Western blotting; animal; dog; dog disease; drug delivery system; evolution; experimental mammary neoplasm; female; genetics; metabolism; udder; Animals; Antineoplastic Agents; Biological Evolution; Dog Diseases; Dogs; Drug Delivery Systems; Female; Mammary Glands, Animal; Mammary Neoplasms, Animal; Phylogeny; Sequestosome-1 Protein
Язык текста: Английский
ISSN: 1476-5829
Mariotti F.
Magi G. E.
Gavazza A.
Vincenzetti S.
Komissarov A.
Shnejder A. M. Aleksandr M 1968-
Venantsi F. Franko 1951-
Мариотти Ф.
Маги Г. Е.
Гавазза А.
Винcензетти С.
Комиссаров А.
Шнейдер А. М. Александр М 1968-
Венанци Ф. Франко 1951-
p62/SQSTM1 expression in canine mammary tumours: Evolutionary notes
Текст визуальный непосредственный
Veterinary and Comparative Oncology
John Wiley & Sons
Vol.17, Issue4 P. 570-577
2019
Статья
autophagy dog gene homology immunohistochemistry mammary tumours p62/SQSTM1
isoprotein recombinant protein sequestosome 1 antineoplastic agent sequestosome 1 adenoma amino acid sequence animal tissue Article autophagy bitch breast tissue breast tumor cancer cell cancer grading Canis chronic inflammation controlled study dog breed epithelium cell epitope mapping histology hyperplasia immunohistochemistry immunoreactivity intraductal papillary mucinous tumor morphology multiple sequence alignment nonhuman papillary carcinoma phylogeny protein expression protein expression level scoring system sequence alignment sequence homology signal transduction Western blotting animal dog dog disease drug delivery system evolution experimental mammary neoplasm female genetics metabolism udder Animals Antineoplastic Agents Biological Evolution Dog Diseases Dogs Drug Delivery Systems Female Mammary Glands, Animal Mammary Neoplasms, Animal Phylogeny Sequestosome-1 Protein
Recent studies highlighted the role of autophagy as a cardinal regulatory system for homeostasis and cancer-related signalling pathways. In this context, the deregulated expression of p62 – Sequestosome1 (p62/SQSTM1) – a protein acting both as an autophagy receptor and signalling hub, has been associated with tumour development and chronic inflammation. Multiple clinical studies test drugs targeting autophagy, and even more research is on the way to clinical trials. However, no comparative investigations have been carried out to identify adequate preclinical models to assess p62-based medicine. In veterinary oncology the role of p62 in cancer-related pathways has been largely ignored. We compared p62 sequences in multiple organisms and found that canine p62 significantly diverges from the humans and from other animals sequences. Then, we chart by immunohistochemistry the expression levels of p62 in canine mammary tumours. A total of 66 tumours and 10 non-neoplastic mammary samples were