Low-dose photodynamic therapy promotes angiogenic potential and increases immunogenicity of human mesenchymal stromal cells
Udartseva O. O., Zhidkova O. V., Ezdakova M. I., Ogneva I. V., Andreeva E. R., Buravkova L. B., Gollnick S. O.
Journal of Photochemistry and Photobiology B: Biology
Vol.199, Num.111596
Опубликовано: 2019
Тип ресурса: Статья
DOI:10.1016/j.jphotobiol.2019.111596
Аннотация:
Photodynamic therapy (PDT) is a non-invasive FDA and EMA-approved anticancer treatment modality. Initially developed for elimination of malignant cells, PDT affects all cells in the tumor bed including stromal cells. Stroma represents not only an important component of tumor microenvironment, but has a significant impact on tumor susceptibility to PDT and other anticancer therapies. However, the effects of PDT on stromal cells are poorly investigated. During PDT the tumor stroma can receive low-dose irradiation as a result of chosen regimen or limited depth of light penetration. Here, we characterized response of human mesenchymal stromal cells (MSCs) to low-dose PDT. In an in vitro model we demonstrated that low-dose PDT resulted in activation of Erk1/2 and inhibition of GSK-3 signaling in MSCs. PDT-mediated induction of intracellular reactive oxygen species (ROS) resulted in reorganization of MSC cytoskeleton and decreased cell motility. More importantly, low-dose PDT dramatically up
Ключевые слова:
angiogenic factor; gelatinase B; glycogen synthase kinase 3; interleukin 8; mitogen activated protein kinase 1; mitogen activated protein kinase 3; monocyte chemotactic protein 1; plasminogen activator inhibitor 1; reactive oxygen metabolite; vasculotropin A; glycogen synthase kinase 3; mitogen activated protein kinase 1; monocyte chemotactic protein 1; photosensitizing agent; reactive oxygen metabolite; angiogenesis; Article; cancer therapy; carcinogenesis; cell activity; cell infiltration; cell motility; cell viability; clinical effectiveness; coculture; controlled study; cytoskeleton; dose response; enzyme activation; enzyme inhibition; human; human cell; human tissue; immunogenicity; immunostimulation; in vitro study; MAPK signaling; mesenchymal stroma cell; photodynamic therapy; priority journal; protein phosphorylation; protein secretion; tumor immunity; upregulation; adipose tissue; animal; cell motion; cell survival; drug effect; embryology; light; low level laser therapy; mese
Язык текста: Английский
ISSN: 1011-1344
Udartseva O. O.
Zhidkova O. V.
Ezdakova M. I.
Ogneva I. V. Irina Vladimirovna 1979-
Andreeva E. R.
Buravkova L. B.
Gollnick S. O.
Ударцева О. О.
Жидкова О. В.
Ездакова М. И.
Огнева И. В. Ирина Владимировна 1979-
Андреева Е. Р.
Буравкова Л. Б.
Голлниcк С. О.
Low-dose photodynamic therapy promotes angiogenic potential and increases immunogenicity of human mesenchymal stromal cells
Текст визуальный непосредственный
Journal of Photochemistry and Photobiology B: Biology
Elsevier Science Publisher B.V.
Vol.199 Num.111596
2019
Статья
angiogenic factor gelatinase B glycogen synthase kinase 3 interleukin 8 mitogen activated protein kinase 1 mitogen activated protein kinase 3 monocyte chemotactic protein 1 plasminogen activator inhibitor 1 reactive oxygen metabolite vasculotropin A glycogen synthase kinase 3 mitogen activated protein kinase 1 monocyte chemotactic protein 1 photosensitizing agent reactive oxygen metabolite angiogenesis Article cancer therapy carcinogenesis cell activity cell infiltration cell motility cell viability clinical effectiveness coculture controlled study cytoskeleton dose response enzyme activation enzyme inhibition human human cell human tissue immunogenicity immunostimulation in vitro study MAPK signaling mesenchymal stroma cell photodynamic therapy priority journal protein phosphorylation protein secretion tumor immunity upregulation adipose tissue animal cell motion cell survival drug effect embryology light low level laser therapy mese
Photodynamic therapy (PDT) is a non-invasive FDA and EMA-approved anticancer treatment modality. Initially developed for elimination of malignant cells, PDT affects all cells in the tumor bed including stromal cells. Stroma represents not only an important component of tumor microenvironment, but has a significant impact on tumor susceptibility to PDT and other anticancer therapies. However, the effects of PDT on stromal cells are poorly investigated. During PDT the tumor stroma can receive low-dose irradiation as a result of chosen regimen or limited depth of light penetration. Here, we characterized response of human mesenchymal stromal cells (MSCs) to low-dose PDT. In an in vitro model we demonstrated that low-dose PDT resulted in activation of Erk1/2 and inhibition of GSK-3 signaling in MSCs. PDT-mediated induction of intracellular reactive oxygen species (ROS) resulted in reorganization of MSC cytoskeleton and decreased cell motility. More importantly, low-dose PDT dramatically up