Experimental Studies of the Expression of Neurotransmitter Transporter Genes in Astrocytes in Different Part of the Brain
Shusharina N. N., Patrushev M. V., Silina E. V., Stupin V. A., Litvitskij P. F., Orlova A. S.
Neuroscience and Behavioral Physiology
Vol.49, Issue7, P. 921-928
Опубликовано: 2019
Тип ресурса: Статья
DOI:10.1007/s11055-019-00820-1
Аннотация:
Objectives. To study the expression of various neurotransmitter transporters (glutamate, aspartate, lactate, choline) in cultures of astrocytes from different part of the brain (cortex, hippocampus, and brainstem) in rats aged three and 11 days. Materials and methods. An experimental study was performed using 24 Rattus norvegicus rats aged three (n = 12) and 11 days (n = 12). High-throughput sequencing results were analyzed. Results. Expression of the glutamate and aspartate transporters in the brainstem in three-day-old rats was greater than in other areas, though the reverse pattern was seen in rats aged 11 days. Expression of the lactate transporter with age was identical to that in the cortex. Conclusions. The data obtained here demonstrated the nature of neuroastrocyte relationships and the role of astrocytes in the process of signal transmission. The results of the study, carried out using original methods of investigating neurotransmitter transporters, allow them to be recommend
Ключевые слова:
aspartate; astrocytes; choline; gene expression; genetic methods of recording neurogenesis; glutamate; lactate; neuroglia; neurotransmitters; transporters
glucose transporter 1; vesicular glutamate transporter 1; animal tissue; Article; astrocyte; brain cortex; brain stem; controlled study; energy consumption; female; gel electrophoresis; gene; gene expression; high throughput sequencing; hippocampus; male; nonhuman; rat; signal transduction; SLC17a7 gene; SLC17ab gene; SLC25a18 gene; SLC2a1 gene; synaptic transmission
Язык текста: Английский
ISSN: 1573-899X
Shusharina N. N.
Patrushev M. V.
Silina E. V. Ekaterina Vladimirovna 1980-
Stupin V. A.
Litvitskij P. F. Petr Frantsevich 1947-
Orlova A. S. Aleksandra Sergeevna 1986-
Шушарина Н. Н.
Патрушев М. В.
Силина Е. В. Екатерина Владимировна 1980-
Стюпин В. А.
Литвицкий П. Ф. Петр Францевич 1947-
Орлова А. С. Александра Сергеевна 1986-
Experimental Studies of the Expression of Neurotransmitter Transporter Genes in Astrocytes in Different Part of the Brain
Текст визуальный непосредственный
Neuroscience and Behavioral Physiology
Springer New York Consultants Bureau
Vol.49, Issue7 P. 921-928
2019
Статья
aspartate astrocytes choline gene expression genetic methods of recording neurogenesis glutamate lactate neuroglia neurotransmitters transporters
glucose transporter 1 vesicular glutamate transporter 1 animal tissue Article astrocyte brain cortex brain stem controlled study energy consumption female gel electrophoresis gene gene expression high throughput sequencing hippocampus male nonhuman rat signal transduction SLC17a7 gene SLC17ab gene SLC25a18 gene SLC2a1 gene synaptic transmission
Objectives. To study the expression of various neurotransmitter transporters (glutamate, aspartate, lactate, choline) in cultures of astrocytes from different part of the brain (cortex, hippocampus, and brainstem) in rats aged three and 11 days. Materials and methods. An experimental study was performed using 24 Rattus norvegicus rats aged three (n = 12) and 11 days (n = 12). High-throughput sequencing results were analyzed. Results. Expression of the glutamate and aspartate transporters in the brainstem in three-day-old rats was greater than in other areas, though the reverse pattern was seen in rats aged 11 days. Expression of the lactate transporter with age was identical to that in the cortex. Conclusions. The data obtained here demonstrated the nature of neuroastrocyte relationships and the role of astrocytes in the process of signal transmission. The results of the study, carried out using original methods of investigating neurotransmitter transporters, allow them to be recommend