HPTLC based approach for bioassay-guided evaluation of antidiabetic and neuroprotective effects of eight essential oils of the Lamiaceae family plants
Romero R. C., Ramasamy K., Meng L. S., Majeed A. B. A., Agatonovich-Kushtrin S.
Journal of Pharmaceutical and Biomedical Analysis
Vol.178, Num.112909
Опубликовано: 2020
Тип ресурса: Статья
DOI:10.1016/j.jpba.2019.112909
Аннотация:
A high-performance thin-layer chromatography (HPTLC) method combined with effect-directed-analysis (EDA) was developed to screen the antioxidant, neuroprotective and antidiabetic effects in essential oils derived from lavender flower, lemon myrtle, oregano, peppermint, sage, and rosemary leaves (Lamiaceae family). HPTLC hyphenated with microchemical (DPPH•, p-anisaldehyde, and ferric chloride) derivatizations, was used to evaluate antioxidant activity, presence of phytosterols and terpenoids, and polyphenolic content, while the combination with biochemical (α-amylase and acetylcholine esterase (AChE) enzymatic) derivatizations was used to asses α-amylase and AChE inhibitory activities. The superior antioxidant activity of oregano leaf extract is attributed to the presence of high levels of aromatic compounds, like polyphenolic acids. The strongest α-amylase inhibition was observed in lemon myrtle and rosemary plus extracts due to the presence of monoterpenes. Rosemary and sage extracts
Ключевые слова:
Acetylcholinesterase inhibitors; Antioxidants; Essential oils; Neuroprotection; Terpenoids; α-Amylase inhibitors
acetylcholinesterase; amylase; antidiabetic agent; essential oil; lavender oil; lemon oil; neuroprotective agent; peppermint oil; phytosterol; plant extract; polyphenol derivative; terpene; terpenoid; antidiabetic agent; antioxidant; biphenyl derivative; cholinesterase inhibitor; essential oil; neuroprotective agent; phenol derivative; plant extract; terpene; antidiabetic activity; antioxidant activity; Article; bioassay; derivatization; DPPH radical scavenging assay; drug synthesis; enzyme inhibition; evaluation study; high performance thin layer chromatography; Lamiaceae; lavender; neuroprotection; nonhuman; oregano; plant leaf; priority journal; rosemary; sage; bioassay; chemistry; high performance liquid chromatography; Lamiaceae; metabolism; procedures; thin layer chromatography; Acetylcholinesterase; alpha-Amylases; Antioxidants; Biological Assay; Biphenyl Compounds; Cholinesterase Inhibitors; Chromatography, High Pressure Liquid; Chromatography, Thin Layer; Hypoglycemic Agents
Язык текста: Английский
ISSN: 1873-264X
Romero R. C. Rocamora C.
Ramasamy K.
Meng L. S. Lim S.
Majeed A. B. A.
Agatonovich-Kushtrin S. Snezhana 1960-
Ромеро Р. C. Роcамора C.
Рамасамy К.
Менг Л. С. Лим С.
Майеед А. Б. А.
Агатонович-Куштрин С. Снежана 1960-
HPTLC based approach for bioassay-guided evaluation of antidiabetic and neuroprotective effects of eight essential oils of the Lamiaceae family plants
Текст визуальный непосредственный
Journal of Pharmaceutical and Biomedical Analysis
Elsevier Science Publisher B.V.
Vol.178 Num.112909
2020
Статья
Acetylcholinesterase inhibitors Antioxidants Essential oils Neuroprotection Terpenoids α-Amylase inhibitors
acetylcholinesterase amylase antidiabetic agent essential oil lavender oil lemon oil neuroprotective agent peppermint oil phytosterol plant extract polyphenol derivative terpene terpenoid antidiabetic agent antioxidant biphenyl derivative cholinesterase inhibitor essential oil neuroprotective agent phenol derivative plant extract terpene antidiabetic activity antioxidant activity Article bioassay derivatization DPPH radical scavenging assay drug synthesis enzyme inhibition evaluation study high performance thin layer chromatography Lamiaceae lavender neuroprotection nonhuman oregano plant leaf priority journal rosemary sage bioassay chemistry high performance liquid chromatography Lamiaceae metabolism procedures thin layer chromatography Acetylcholinesterase alpha-Amylases Antioxidants Biological Assay Biphenyl Compounds Cholinesterase Inhibitors Chromatography, High Pressure Liquid Chromatography, Thin Layer Hypoglycemic Agents
A high-performance thin-layer chromatography (HPTLC) method combined with effect-directed-analysis (EDA) was developed to screen the antioxidant, neuroprotective and antidiabetic effects in essential oils derived from lavender flower, lemon myrtle, oregano, peppermint, sage, and rosemary leaves (Lamiaceae family). HPTLC hyphenated with microchemical (DPPH•, p-anisaldehyde, and ferric chloride) derivatizations, was used to evaluate antioxidant activity, presence of phytosterols and terpenoids, and polyphenolic content, while the combination with biochemical (α-amylase and acetylcholine esterase (AChE) enzymatic) derivatizations was used to asses α-amylase and AChE inhibitory activities. The superior antioxidant activity of oregano leaf extract is attributed to the presence of high levels of aromatic compounds, like polyphenolic acids. The strongest α-amylase inhibition was observed in lemon myrtle and rosemary plus extracts due to the presence of monoterpenes. Rosemary and sage extracts