Аннотация:
Here we report sequence and phylogenetic analysis of two new isolates of Leishmania RNA virus 2 (LRV2) found in Leishmania major isolated from human patients with cutaneous leishmaniasis in south Uzbekistan. These new virus-infected flagellates were isolated in the same region of Uzbekistan and the viral sequences differed by only nineteen SNPs, all except one being silent mutations. Therefore, we concluded that they belong to a single LRV2 species. New viruses are closely related to the LRV2-Lmj-ASKH documented in Turkmenistan in 1995, which is congruent with their shared host (L. major) and common geographical origin.
Ключевые слова:
Leishmania RNA virus; LRV2; next-generation sequencing
classification; genetics; Leishmania major; Leishmaniavirus; mutation; pathogenicity; phylogeny; single nucleotide polymorphism; Uzbekistan; virology; virus genome; Genome, Viral; Leishmania major; Leishmaniavirus; Mutation; Phylogeny; Polymorphism, Single Nucleotide; Uzbekistan
Kleshhenko Yu. E. Yuliya Evgenyevna 1978-
Grybchuk D.
Matveeva N. S. Nadezhda Sergeevna 1998-
Macedo D. H.
Ponirovskij E. N. Evgenij Nikolaevich 1939-
Lukashev A. N. Aleksandr Nikolaevich 1977-
Yurchenko V. Vyacheslav 1972-
Клещенко Ю. Е. Юлия Евгеньевна 1978-
Грyбчук Д.
Матвеева Н. С. Надежда Сергеевна 1998-
Маcедо Д. Х.
Понировский Е. Н. Евгений Николаевич 1939-
Лукашев А. Н. Александр Николаевич 1977-
Юрченко В. Вячеслав 1972-
Molecular Characterization of Leishmania RNA virus 2 in Leishmaniamajor from Uzbekistan
Текст визуальный непосредственный
Genes
Vol.10, Issue10
2019
Статья
Leishmania RNA virus LRV2 next-generation sequencing
classification genetics Leishmania major Leishmaniavirus mutation pathogenicity phylogeny single nucleotide polymorphism Uzbekistan virology virus genome Genome, Viral Leishmania major Leishmaniavirus Mutation Phylogeny Polymorphism, Single Nucleotide Uzbekistan
Here we report sequence and phylogenetic analysis of two new isolates of Leishmania RNA virus 2 (LRV2) found in Leishmania major isolated from human patients with cutaneous leishmaniasis in south Uzbekistan. These new virus-infected flagellates were isolated in the same region of Uzbekistan and the viral sequences differed by only nineteen SNPs, all except one being silent mutations. Therefore, we concluded that they belong to a single LRV2 species. New viruses are closely related to the LRV2-Lmj-ASKH documented in Turkmenistan in 1995, which is congruent with their shared host (L. major) and common geographical origin.