Hydroxyurea-loaded albumin nanoparticles: Preparation, characterization, and in vitro studies
Tazhbayev Y., Mukashev O., Burkeev M., Kreuter J.
Pharmaceutics
Vol.11, Issue8, Num.410
Опубликовано: 2019
Тип ресурса: Статья
DOI:10.3390/pharmaceutics11080410
Аннотация:
Human serum albumin nanoparticles (HSA-NPs) have been widely used as drug delivery systems. In most cases, HSA-NPs are formed by the method of desolvation in the presence of glutaraldehyde as a crosslinking agent. In the present study, we showed the possibility of crosslinking human serum albumin (HSA) molecules with natural agents, urea, and cysteine at the nanoparticle level under mild conditions (at room temperature of 20–25 °C). Optimal concentrations of the interacting components (HSA, urea, and cysteine) were found to produce nanoparticles with optimal physico-chemical parameters (particle size, polydispersity, zeta potential, yield, etc.) for application as drug carriers. We used hydroxyurea (HU), a simple organic compound currently used as a cancer chemotherapeutic agent. The results indicated sizes of 196 ± 5 nm and 288 ± 10 nm with a surface charge of −22 ± 3.4 mV and −17.4 ± 0.5 mV for HSA-NPs (20 mg/mL of HSA, 0.01 mg/mL of cysteine, and 10 mg/mL of urea) and HSA–HU-NPs (2
Ключевые слова:
Cancer; Human serum albumin; Hydroxyurea; Nanoparticles
albumin nanoparticle; cysteine; human serum albumin; hydroxyurea; nanoparticle; organic compound; unclassified drug; urea; Article; controlled study; cross linking; dispersity; drug release; in vitro study; nanoencapsulation; particle size; physical chemistry; process optimization; room temperature; surface property; zeta potential
Язык текста: Английский
ISSN: 1999-4923
Tazhbayev Y.
Mukashev O.
Burkeev M.
Kreuter J.
Тажбайев Y.
Мукашев О.
Буркеев М.
Креутер Й.
Hydroxyurea-loaded albumin nanoparticles: Preparation, characterization, and in vitro studies
Текст визуальный непосредственный
Pharmaceutics
Vol.11, Issue8 Num.410
2019
Статья
Cancer Human serum albumin Hydroxyurea Nanoparticles
albumin nanoparticle cysteine human serum albumin hydroxyurea nanoparticle organic compound unclassified drug urea Article controlled study cross linking dispersity drug release in vitro study nanoencapsulation particle size physical chemistry process optimization room temperature surface property zeta potential
Human serum albumin nanoparticles (HSA-NPs) have been widely used as drug delivery systems. In most cases, HSA-NPs are formed by the method of desolvation in the presence of glutaraldehyde as a crosslinking agent. In the present study, we showed the possibility of crosslinking human serum albumin (HSA) molecules with natural agents, urea, and cysteine at the nanoparticle level under mild conditions (at room temperature of 20–25 °C). Optimal concentrations of the interacting components (HSA, urea, and cysteine) were found to produce nanoparticles with optimal physico-chemical parameters (particle size, polydispersity, zeta potential, yield, etc.) for application as drug carriers. We used hydroxyurea (HU), a simple organic compound currently used as a cancer chemotherapeutic agent. The results indicated sizes of 196 ± 5 nm and 288 ± 10 nm with a surface charge of −22 ± 3.4 mV and −17.4 ± 0.5 mV for HSA-NPs (20 mg/mL of HSA, 0.01 mg/mL of cysteine, and 10 mg/mL of urea) and HSA–HU-NPs (2