Testing Safety of Genetically Modified Products of Rice: Case Study on Sprague Dawley Rats
Shirdeli M., Orlov Yu. L., Eslami G., Hajimohammadi B., Tabikhanova L. E., Ehrampoush M. H., Rezvani M. E., Fallahzadeh H., Zandi H., Hosseini S., Ahmadian S., Mortazavi S., Fallahi R., Asadi-Yousefabad S.
Russian Journal of Genetics
Vol.55, Issue8, P. 962-968
Опубликовано: 2019
Тип ресурса: Статья
DOI:10.1134/S1022795419080131
Аннотация:
Abstract: Genetic engineering is considered as background for crop protection against pest damage by adding new genes inside the grains. Rice, like other cereals is included in gene engineering experiments. The questions about possible gene transfer related to food safety appear. It is important to find any additional genes or fragments in animal tissues after consumption of genetically modified (GM) food. Therefore, in this study, the remaining of CryIA(b) gene and P35 were assessed in the liver of rats fed with GM rice. This work presents an experimental study with the intervention of GM rice feeding by Sprague Dawley rats. Overall, 20 male and 20 female SD rats were fed by pellets made by GM rice in 50[%] of needed carbohydrate for 90 days. Then, sampling was done from rats liver. DNA extraction was done based on the protocol. The quality and quantity of the extracted DNA was done by agarose gel electrophoresis and spectrophotometry, respectively. Detection of GM genes residues, inclu
Ключевые слова:
CryIA(b); food safety; genetically modified rice; liver; P35; Sprague Dawley rat; T35; transgene
carbohydrate; ketamine; protein p35; agar gel electrophoresis; animal experiment; animal model; animal tissue; Article; Bacillus thuringiensis; controlled study; cryIAb gene; DNA extraction; feeding; female; food safety; gene; gene transfer; genetic engineering; genetic screening; genetically modified food; liver tissue; male; nonhuman; polymerase chain reaction; priority journal; rat; rice; spectrophotometry; T35 gene; transgenic crop
Язык текста: Английский
ISSN: 1608-3369
Shirdeli M.
Orlov Yu. L. Yurij L`vovich 1967-
Eslami G.
Hajimohammadi B.
Tabikhanova L. E.
Ehrampoush M. H.
Rezvani M. E.
Fallahzadeh H.
Zandi H.
Hosseini S.
Ahmadian S.
Mortazavi S.
Fallahi R.
Asadi-Yousefabad S.
Ширдели М.
Орлов Ю. Л. Юрий Львович 1967-
Еслами Г.
Хайимохаммади Б.
Табиханова Л. Е.
Ехрампоуш М. Х.
Резвани М. Е.
Фаллахзадех Х.
Занди Х.
Хоссеини С.
Ахмадиан С.
Мортазави С.
Фаллахи Р.
Асади-Ёусефабад С.
Testing Safety of Genetically Modified Products of Rice: Case Study on Sprague Dawley Rats
Текст визуальный непосредственный
Russian Journal of Genetics
Pleiades Publishing, Inc.
Vol.55, Issue8 P. 962-968
2019
Статья
CryIA(b) food safety genetically modified rice liver P35 Sprague Dawley rat T35 transgene
carbohydrate ketamine protein p35 agar gel electrophoresis animal experiment animal model animal tissue Article Bacillus thuringiensis controlled study cryIAb gene DNA extraction feeding female food safety gene gene transfer genetic engineering genetic screening genetically modified food liver tissue male nonhuman polymerase chain reaction priority journal rat rice spectrophotometry T35 gene transgenic crop
Abstract: Genetic engineering is considered as background for crop protection against pest damage by adding new genes inside the grains. Rice, like other cereals is included in gene engineering experiments. The questions about possible gene transfer related to food safety appear. It is important to find any additional genes or fragments in animal tissues after consumption of genetically modified (GM) food. Therefore, in this study, the remaining of CryIA(b) gene and P35 were assessed in the liver of rats fed with GM rice. This work presents an experimental study with the intervention of GM rice feeding by Sprague Dawley rats. Overall, 20 male and 20 female SD rats were fed by pellets made by GM rice in 50[%] of needed carbohydrate for 90 days. Then, sampling was done from rats liver. DNA extraction was done based on the protocol. The quality and quantity of the extracted DNA was done by agarose gel electrophoresis and spectrophotometry, respectively. Detection of GM genes residues, inclu