Effect of the Surface Microtopography of Titanium Matrices on the Proliferation and Viability of Fibroblast-Like Cells
Shaykhaliev A. I., Polisan A. A., Ivanov S. Yu., Parkhomenko Y. N., Malinkovich M. D., Yarygin K. N.
Journal of Surface Investigation: X-Ray, Synchrotron and Neutron Techniques
Vol.13, Issue4, P. 744-751
Опубликовано: 2019
Тип ресурса: Статья
DOI:10.1134/S1027451019040335
Аннотация:
Abstract: We study the effect of the microtopography of the surface of titanium implants on the proliferation (reproduction process) of fibroblast-like cells (cells of the connective tissue of the body) using the morphological analysis of cell cultures by phase-contrast microscopy. Three-dimensional titanium cell matrices (scaffolds) are used as the mechanical frame for the analyzed cells. The metabolic activity of the cells, cell viability, and the cytotoxic effect of scaffolds on human-skin fibroblast cultures are evaluated using the colorimetric MTT test. Titanium scaffolds with a polished surface and with a sandblasted surface and the same samples with a bioactive titanium-dioxide coating with the anatase structure are used in the experiments. The morphological analysis of human-skin fibroblasts in the presence of scaffolds does not show any changes in the appearance of the cells compared with control cells cultured in the absence of scaffolds. Evaluation of the cytotoxic effect of
Ключевые слова:
colorimetric MTT test; fibroblast-like cells; microtopography of the surface; morphological analysis of cell cultures; phase-contrast microscopy; profilometry; proliferation; three-dimensional matrices (scaffolds); titanium implant
Cell culture; Cell death; Cell proliferation; Collagen; Colorimetric analysis; Colorimetry; Fibroblasts; Laser tissue interaction; Linguistics; Metal implants; Musculoskeletal system; Profilometry; Surface treatment; Tissue; Titanium dioxide; Micro topography; Morphological analysis; Phase-contrast microscopy; proliferation; Three dimensional matrices; Titanium implants; Scaffolds (biology)
Язык текста: Английский
ISSN: 1819-7094
Shaykhaliev A. I. Astemir Ikramovich 1965-
Polisan A. A.
Ivanov S. Yu. Sergej Yuryevich 1957-
Parkhomenko Y. N.
Malinkovich M. D.
Yarygin K. N.
Шайхалиев А. И. Астемир Икрамович 1965-
Полисан А. А.
Иванов С. Ю. Сергей Юрьевич 1957-
Пархоменко Y. Н.
Малинкович М. Д.
Ярyгин К. Н.
Effect of the Surface Microtopography of Titanium Matrices on the Proliferation and Viability of Fibroblast-Like Cells
Текст визуальный непосредственный
Journal of Surface Investigation: X-Ray, Synchrotron and Neutron Techniques
Pleiades Publishing, Ltd.
Vol.13, Issue4 P. 744-751
2019
Статья
colorimetric MTT test fibroblast-like cells microtopography of the surface morphological analysis of cell cultures phase-contrast microscopy profilometry proliferation three-dimensional matrices (scaffolds) titanium implant
Cell culture Cell death Cell proliferation Collagen Colorimetric analysis Colorimetry Fibroblasts Laser tissue interaction Linguistics Metal implants Musculoskeletal system Profilometry Surface treatment Tissue Titanium dioxide Micro topography Morphological analysis Phase-contrast microscopy proliferation Three dimensional matrices Titanium implants Scaffolds (biology)
Abstract: We study the effect of the microtopography of the surface of titanium implants on the proliferation (reproduction process) of fibroblast-like cells (cells of the connective tissue of the body) using the morphological analysis of cell cultures by phase-contrast microscopy. Three-dimensional titanium cell matrices (scaffolds) are used as the mechanical frame for the analyzed cells. The metabolic activity of the cells, cell viability, and the cytotoxic effect of scaffolds on human-skin fibroblast cultures are evaluated using the colorimetric MTT test. Titanium scaffolds with a polished surface and with a sandblasted surface and the same samples with a bioactive titanium-dioxide coating with the anatase structure are used in the experiments. The morphological analysis of human-skin fibroblasts in the presence of scaffolds does not show any changes in the appearance of the cells compared with control cells cultured in the absence of scaffolds. Evaluation of the cytotoxic effect of