Upregulation of PD-L1 expression in breast cancer cells through the formation of 3D multicellular cancer aggregates under different chemical and...
Azadi S., Aboulkheyr E. H., Razavi B. S., Tyeri Zh. P. R., Asadnia M., Ibragimi V. M. M.
Biochimica et Biophysica Acta (BBA) - Molecular Cell Research
Vol.1866, Issue12, Num.118526
Опубликовано: 2019
Тип ресурса: Статья
DOI:10.1016/j.bbamcr.2019.118526
Аннотация:
Expression of programmed death-ligand 1 (PD-L1) in cancer cells plays an important role in cancer-immune cell interaction. The emerging evidence suggests regulation of PD-L1 expression by several tumor microenvironmental cues. However, the association of PD-L1 expression with chemical and mechanical features of the tumor microenvironment, specifically epidermal growth factor receptor (EGFR) signaling and matrix stiffness, remains elusive. Herein, we determine whether EGFR targeting and substrate stiffness affect the regulation of PD-L1 expression. Breast carcinoma cell lines, MCF7 and MDA-MB-231, were cultured under different conditions targeting EGFR and exposing cells to distinct substrate stiffness to evaluate PD-L1 expression. Furthermore, the ability to form aggregates in short-term culture of breast carcinoma cells and its effect on expression level of PD-L1 was probed. Our results indicated that PD-L1 expression was altered in response to both EGFR inhibition and substrate stiff
Ключевые слова:
Cetuximab; EGFR; Immunotherapy; PD-L1; Substrate stiffness
cetuximab; epidermal growth factor receptor; programmed death 1 ligand 1; transcription factor; CD274 protein, human; EGFR protein, human; epidermal growth factor receptor; programmed death 1 ligand 1; Article; breast carcinoma; enzyme linked immunosorbent assay; epithelial mesenchymal transition; human; human cell; MCF-7 cell line; MDA-MB-231 cell line; priority journal; protein expression; signal transduction; tumor microenvironment; upregulation; biosynthesis; breast tumor; female; metabolism; pathology; tumor cell culture; upregulation; B7-H1 Antigen; Breast Neoplasms; ErbB Receptors; Female; Humans; MCF-7 Cells; Signal Transduction; Tumor Cells, Cultured; Tumor Microenvironment; Up-Regulation
Язык текста: Английский
ISSN: 0167-4889
Azadi S.
Aboulkheyr E. H. Es H.
Razavi B. S. Bazaz S.
Tyeri Zh. P. R. Zhan Pol` Rodzher 1947-
Asadnia M.
Ibragimi V. M. M. Varkiani Madzhid Modzhtaba 1983-
Азади С.
Абоулхеyр Е. Х. Ес Х.
Разави Б. С. Базаз С.
Тьери Ж. П. Р. Жан Поль Роджер 1947-
Асадниа М.
Ибрагими В. М. М. Варкиани Маджид Моджтаба 1983-
Upregulation of PD-L1 expression in breast cancer cells through the formation of 3D multicellular cancer aggregates under different chemical and mechanical conditions
Upregulation of PD-L1 expression in breast cancer cells through the formation of 3D multicellular cancer aggregates under different chemical and...
Текст визуальный непосредственный
Biochimica et Biophysica Acta (BBA) - Molecular Cell Research
Elsevier Science Publisher B.V.
Vol.1866, Issue12 Num.118526
2019
Статья
Cetuximab EGFR Immunotherapy PD-L1 Substrate stiffness
cetuximab epidermal growth factor receptor programmed death 1 ligand 1 transcription factor CD274 protein, human EGFR protein, human epidermal growth factor receptor programmed death 1 ligand 1 Article breast carcinoma enzyme linked immunosorbent assay epithelial mesenchymal transition human human cell MCF-7 cell line MDA-MB-231 cell line priority journal protein expression signal transduction tumor microenvironment upregulation biosynthesis breast tumor female metabolism pathology tumor cell culture upregulation B7-H1 Antigen Breast Neoplasms ErbB Receptors Female Humans MCF-7 Cells Signal Transduction Tumor Cells, Cultured Tumor Microenvironment Up-Regulation
Expression of programmed death-ligand 1 (PD-L1) in cancer cells plays an important role in cancer-immune cell interaction. The emerging evidence suggests regulation of PD-L1 expression by several tumor microenvironmental cues. However, the association of PD-L1 expression with chemical and mechanical features of the tumor microenvironment, specifically epidermal growth factor receptor (EGFR) signaling and matrix stiffness, remains elusive. Herein, we determine whether EGFR targeting and substrate stiffness affect the regulation of PD-L1 expression. Breast carcinoma cell lines, MCF7 and MDA-MB-231, were cultured under different conditions targeting EGFR and exposing cells to distinct substrate stiffness to evaluate PD-L1 expression. Furthermore, the ability to form aggregates in short-term culture of breast carcinoma cells and its effect on expression level of PD-L1 was probed. Our results indicated that PD-L1 expression was altered in response to both EGFR inhibition and substrate stiff