The influence of folic acid-induced acute kidney injury on cardiac function and redox status in rats

Nikolic T., Petrovic D., Matic S., Turnic T. N., Jeremic J., Radonjic K., Srejovic I., Zivkovic V., Bolevich S., Yakovlevich V.

Naunyn-Schmiedeberg's Archives of Pharmacology

Vol.393, Issue1, P. 99-109

Опубликовано: 2020

Тип ресурса: Статья

DOI:10.1007/s00210-019-01717-z

Аннотация:

The aim of this study was to evaluate the influence of acute kidney injury caused by high doses of folic acid on cardiac function and markers of oxidative stress in serum and isolated rat heart. Isolated hearts of Wistar albino rats (divided into two groups: control and folic acid group) were perfused according to Langendorff technique at basal coronary perfusion pressure CPP of 70 cm H2O. After a stabilization period, CPP was lowered to 60 cm H2O and then gradually increased to 80, 100, 120 and finally decreased to 40 cm H2O in order to establish coronary autoregulation. For each perfusion pressure value, the left ventricular function parameters were determined. Samples of coronary venous effluent were collected for determination of coronary flow and biomarkers of oxidative stress. The blood samples were collected in order to examine the values of serum urea, creatinine, Na, K, and parameters of oxidative stress and antioxidant defense system. Heart and kidney tissue samples were coll

Ключевые слова:

Acute kidney injury; Cardiac function; Folic acid; Oxidative stress

creatinine; folic acid; hydrogen peroxide; nitric oxide; sodium; thiobarbituric acid reactive substance; urea; acute kidney failure; animal experiment; animal model; animal tissue; Article; blood sampling; controlled study; coronary artery blood flow; creatinine blood level; diastolic blood pressure; heart function; heart left ventricle function; heart left ventricle pressure; heart tissue; histopathology; kidney tissue; male; nonhuman; oxidation reduction reaction; oxidative stress; potassium blood level; rat; sodium blood level; systolic blood pressure; urea blood level

Язык текста: Английский

ISSN: 1432-1912

Nikolic T.

Petrovic D.

Matic S.

Turnic T. N.

Jeremic J.

Radonjic K.

Srejovic I.

Zivkovic V.

Bolevich S. Sergej 1964-

Yakovlevich V. Vladimir 1971-

Николиc Т.

Петровиc Д.

Матиc С.

Тюрниc Т. Н.

Йеремиc Й.

Радонйиc К.

Срейовиc И.

Зивковиc В.

Болевич С. Сергей 1964-

Яковлевич В. Владимир 1971-

The influence of folic acid-induced acute kidney injury on cardiac function and redox status in rats

Текст визуальный непосредственный

Naunyn-Schmiedeberg's Archives of Pharmacology

Springer-Verlag GmbH

Vol.393, Issue1 P. 99-109

2020

Статья

Acute kidney injury Cardiac function Folic acid Oxidative stress

creatinine folic acid hydrogen peroxide nitric oxide sodium thiobarbituric acid reactive substance urea acute kidney failure animal experiment animal model animal tissue Article blood sampling controlled study coronary artery blood flow creatinine blood level diastolic blood pressure heart function heart left ventricle function heart left ventricle pressure heart tissue histopathology kidney tissue male nonhuman oxidation reduction reaction oxidative stress potassium blood level rat sodium blood level systolic blood pressure urea blood level

The aim of this study was to evaluate the influence of acute kidney injury caused by high doses of folic acid on cardiac function and markers of oxidative stress in serum and isolated rat heart. Isolated hearts of Wistar albino rats (divided into two groups: control and folic acid group) were perfused according to Langendorff technique at basal coronary perfusion pressure CPP of 70 cm H2O. After a stabilization period, CPP was lowered to 60 cm H2O and then gradually increased to 80, 100, 120 and finally decreased to 40 cm H2O in order to establish coronary autoregulation. For each perfusion pressure value, the left ventricular function parameters were determined. Samples of coronary venous effluent were collected for determination of coronary flow and biomarkers of oxidative stress. The blood samples were collected in order to examine the values of serum urea, creatinine, Na, K, and parameters of oxidative stress and antioxidant defense system. Heart and kidney tissue samples were coll