Proteome of HU-Lacking E. coli Studied by Means of 2D Gel Electrophoresis
Kamashev D. E`., Rakitina T. V., Matyushkina D. S., Evsyutina D. V., Vanyushkina A. A., Agapova Y. K., Anisimova V. E., Droby'shev A. L., Butenko I. O., Pobeguts O. V., Fisunov G. Y.
Russian Journal of Bioorganic Chemistry
Vol.45, Issue5, P. 366-373
Опубликовано: 2019
Тип ресурса: Статья
DOI:10.1134/S1068162019050029
Аннотация:
Abstract: Histone-like protein HU is a dimeric nucleoid-associated protein (NAP). HU is the most conserved NAP. It binds nonspecifically to duplex DNA with a preference for targeting nicked and bent DNA. HU limits the architecture of the bacterial nucleoid and its deletion is lethal for Bacillus subtilis and Mycoplasma genitalium which do not contain other NAPs. E. coli lacking HU is viable but has numerous growth defects. The effects of the HU protein on gene expression is known from microarray analysis and HU regulons were identified. In HU-deficient E. coli, absence of this DNA architectural protein causes a disorder in gene regulation; on the other hand, E. coli growth under standard conditions is almost unaltered in the absence of HU. To understand how the bacterium confronts the chromosomal disorder, we performed proteome analysis to compare protein abundances in cells containing the HU protein or not. Comparison of the proteomic profile of wild-type and HU-deficient E. coli show
Ключевые слова:
comparative proteome analysis; expression regulation; histone-like HU protein; two-dimensional difference gel electrophoresis
Язык текста: Английский
ISSN: 1068-330X
Kamashev D. E`. Dmitrij E`duardovich 1964-
Rakitina T. V.
Matyushkina D. S.
Evsyutina D. V.
Vanyushkina A. A.
Agapova Y. K.
Anisimova V. E. Veronika Evgenyevna 1978-
Droby'shev A. L. Aleksej Leonidovich 1970-
Butenko I. O.
Pobeguts O. V.
Fisunov G. Y.
Камашев Д. Э. Дмитрий Эдуардович 1964-
Ракитина Т. В.
Матюшкина Д. С.
Евсютина Д. В.
Ванюшкина А. А.
Агапова Y. К.
Анисимова В. Е. Вероника Евгеньевна 1978-
Дробышев А. Л. Алексей Леонидович 1970-
Бутенко И. О.
Побегуц О. В.
Фисунов Г. Y.
Proteome of HU-Lacking E. coli Studied by Means of 2D Gel Electrophoresis
Текст визуальный непосредственный
Russian Journal of Bioorganic Chemistry
Pleiades Publishing, Ltd.
Vol.45, Issue5 P. 366-373
2019
Статья
comparative proteome analysis expression regulation histone-like HU protein two-dimensional difference gel electrophoresis
Abstract: Histone-like protein HU is a dimeric nucleoid-associated protein (NAP). HU is the most conserved NAP. It binds nonspecifically to duplex DNA with a preference for targeting nicked and bent DNA. HU limits the architecture of the bacterial nucleoid and its deletion is lethal for Bacillus subtilis and Mycoplasma genitalium which do not contain other NAPs. E. coli lacking HU is viable but has numerous growth defects. The effects of the HU protein on gene expression is known from microarray analysis and HU regulons were identified. In HU-deficient E. coli, absence of this DNA architectural protein causes a disorder in gene regulation; on the other hand, E. coli growth under standard conditions is almost unaltered in the absence of HU. To understand how the bacterium confronts the chromosomal disorder, we performed proteome analysis to compare protein abundances in cells containing the HU protein or not. Comparison of the proteomic profile of wild-type and HU-deficient E. coli show