Effects of sterols on the interaction of SDS, benzalkonium chloride, and a novel compound, Kor105, with membranes
Jiménez-Munguía I., Volynsky P. E., Batishchev O. V., Akimov S. A., Korshunova G. A., Smirnova E. A., Knorre D. A., Sokolov S. S., Severin F. F.
Biomolecules
Vol.9, Issue10, Num.627
Опубликовано: 2019
Тип ресурса: Статья
Аннотация:
Sterols change the biophysical properties of lipid membranes. Here, we analyzed how sterols affect the activity of widely used antimicrobial membrane-active compounds, sodium dodecyl sulfate (SDS) and benzalkonium chloride (BAC). We also tested a novel benzalkonium-like substance, Kor105. Our data suggest that benzalkonium and Kor105 disturb the ordering of the membrane lipid packaging, and this disturbance is dampened by cholesterol. The disturbance induced by Kor105 is stronger than that induced by BAC because of the higher rigidity of the Kor105 molecule due to a shorter linker between the phenyl group and quaternary nitrogen. On the contrary, individual SDS molecules do not cause the disturbance. Thus, in the tested range of concentrations, SDS-membrane interaction is not influenced by cholesterol. To study how sterols influence the biological effects of these chemicals, we used yeast strains lacking Lam1-4 proteins. These proteins transport sterols from the plasma membrane into th
Ключевые слова:
Inner membrane field compensation; Ionic surfactant; Lipid membrane; Molecular dynamics; Sterol; Yeast
amphotericin B; benzalkonium; benzalkonium chloride; bromine derivative; dodecyl sulfate sodium; ergosterol; sterol; tetradecyl dimethyl ethyl ammonium bromide; unclassified drug; Article; biophysics; cell membrane; controlled study; electricity; endoplasmic reticulum; gene deletion; growth rate; human; hydrophobicity; lipid membrane; optical density; protein interaction; Saccharomyces cerevisiae; screening
Язык текста: Английский
ISSN: 2218-273X
Jiménez-Munguía I.
Volynsky P. E.
Batishchev O. V.
Akimov S. A.
Korshunova G. A.
Smirnova E. A.
Knorre D. A. Dmitrij Alekseevich 1979-
Sokolov S. S.
Severin F. F.
Йимéнез-Мунгуíа И.
Волyнскy П. Е.
Батишчев О. В.
Акимов С. А.
Коршунова Г. А.
Смирнова Е. А.
Кнорре Д. А. Дмитрий Алексеевич 1979-
Соколов С. С.
Северин Ф. Ф.
Effects of sterols on the interaction of SDS, benzalkonium chloride, and a novel compound, Kor105, with membranes
Текст визуальный непосредственный
Biomolecules
Vol.9, Issue10 Num.627
2019
Статья
Inner membrane field compensation Ionic surfactant Lipid membrane Molecular dynamics Sterol Yeast
amphotericin B benzalkonium benzalkonium chloride bromine derivative dodecyl sulfate sodium ergosterol sterol tetradecyl dimethyl ethyl ammonium bromide unclassified drug Article biophysics cell membrane controlled study electricity endoplasmic reticulum gene deletion growth rate human hydrophobicity lipid membrane optical density protein interaction Saccharomyces cerevisiae screening
Sterols change the biophysical properties of lipid membranes. Here, we analyzed how sterols affect the activity of widely used antimicrobial membrane-active compounds, sodium dodecyl sulfate (SDS) and benzalkonium chloride (BAC). We also tested a novel benzalkonium-like substance, Kor105. Our data suggest that benzalkonium and Kor105 disturb the ordering of the membrane lipid packaging, and this disturbance is dampened by cholesterol. The disturbance induced by Kor105 is stronger than that induced by BAC because of the higher rigidity of the Kor105 molecule due to a shorter linker between the phenyl group and quaternary nitrogen. On the contrary, individual SDS molecules do not cause the disturbance. Thus, in the tested range of concentrations, SDS-membrane interaction is not influenced by cholesterol. To study how sterols influence the biological effects of these chemicals, we used yeast strains lacking Lam1-4 proteins. These proteins transport sterols from the plasma membrane into th