Poliovirus-binding inhibition ELISA based on specific chicken egg yolk antibodies as an alternative to the neutralization test
Ivanov A. P., Klebleyeva T. D., Malyshkina L. P., Ivanova O. E.
Journal of Virological Methods
Vol.266, P. 7-10
Опубликовано: 2019
Тип ресурса: Статья
DOI:10.1016/j.jviromet.2019.01.007
Аннотация:
The first application results of enzyme immunoassay system (ELISA) – binding inhibition ELISA (BI ELISA) for the detection of antibodies to polioviruses of three types based on the use of specific antibodies from chicken yolk (IgY) are presented. This variant of ELISA is a “surrogate” neutralization test (NT). When comparing the results of the detection of antibodies in 90 sera of children who were vaccinated with oral and inactivated poliovirus vaccines, good correlation (r = 0.67, 0.61, 0.76 for types 1, 2, 3 respectively) between the BI ELISA and the NT results was shown. The presented variant of ELISA is the further stage of the development of “IgY-technology” for laboratory diagnostics of viral infections, namely poliovirus infection, which can be used for seroepidemiological studies as an analogue of the NT which requires the use of life poliovirus with the required standard of biosafety containment facilities. © 2019 Elsevier B.V.
Ключевые слова:
Binding inhibition ELISA; IgY; Neutralization test; Polio eradication
immunoglobulin Y; inactivated vaccine; oral poliomyelitis vaccine; poliomyelitis vaccine; immunoglobulin; immunoglobulin Y; oral poliomyelitis vaccine; poliomyelitis vaccine; virus antibody; antibody detection; antibody specificity; Article; binding inhibition enzyme linked immunosorbent assay; chicken; correlation analysis; enzyme linked immunosorbent assay; human; intermethod comparison; nonhuman; poliomyelitis; Poliomyelitis virus; priority journal; virus neutralization; animal; blood; egg yolk; female; immunology; male; Poliomyelitis virus; preschool child; serodiagnosis; virus attachment; Animals; Antibodies, Viral; Chickens; Child, Preschool; Egg Yolk; Enzyme-Linked Immunosorbent Assay; Female; Humans; Immunoglobulins; Male; Neutralization Tests; Poliovirus; Poliovirus Vaccine, Inactivated; Poliovirus Vaccine, Oral; Virus Attachment
Язык текста: Английский
ISSN: 1879-0984
Ivanov A. P.
Klebleyeva T. D.
Malyshkina L. P.
Ivanova O. E. Ol`ga Evgenyevna 1955-
Иванов А. П.
Клеблеьева Т. Д.
Малyшкина Л. П.
Иванова О. Е. Ольга Евгеньевна 1955-
Poliovirus-binding inhibition ELISA based on specific chicken egg yolk antibodies as an alternative to the neutralization test
Текст визуальный непосредственный
Journal of Virological Methods
Elsevier Science Publisher B.V.
Vol.266 P. 7-10
2019
Статья
Binding inhibition ELISA IgY Neutralization test Polio eradication
immunoglobulin Y inactivated vaccine oral poliomyelitis vaccine poliomyelitis vaccine immunoglobulin immunoglobulin Y oral poliomyelitis vaccine poliomyelitis vaccine virus antibody antibody detection antibody specificity Article binding inhibition enzyme linked immunosorbent assay chicken correlation analysis enzyme linked immunosorbent assay human intermethod comparison nonhuman poliomyelitis Poliomyelitis virus priority journal virus neutralization animal blood egg yolk female immunology male Poliomyelitis virus preschool child serodiagnosis virus attachment Animals Antibodies, Viral Chickens Child, Preschool Egg Yolk Enzyme-Linked Immunosorbent Assay Female Humans Immunoglobulins Male Neutralization Tests Poliovirus Poliovirus Vaccine, Inactivated Poliovirus Vaccine, Oral Virus Attachment
The first application results of enzyme immunoassay system (ELISA) – binding inhibition ELISA (BI ELISA) for the detection of antibodies to polioviruses of three types based on the use of specific antibodies from chicken yolk (IgY) are presented. This variant of ELISA is a “surrogate” neutralization test (NT). When comparing the results of the detection of antibodies in 90 sera of children who were vaccinated with oral and inactivated poliovirus vaccines, good correlation (r = 0.67, 0.61, 0.76 for types 1, 2, 3 respectively) between the BI ELISA and the NT results was shown. The presented variant of ELISA is the further stage of the development of “IgY-technology” for laboratory diagnostics of viral infections, namely poliovirus infection, which can be used for seroepidemiological studies as an analogue of the NT which requires the use of life poliovirus with the required standard of biosafety containment facilities. © 2019 Elsevier B.V.