Hydrogen peroxide in the reactions of cancer cells to cisplatin
Nerush A. S., Шсхукина К. М., Balalaeva I. V., Orlova A. G.
Biochimica et Biophysica Acta (BBA)/General Subjects
Vol.1863, Issue4, P. 692-702
Опубликовано: 2019
Тип ресурса: Статья
DOI:10.1016/j.bbagen.2019.01.013
Аннотация:
Background: Hydrogen peroxide (H 2 O 2 ) is thought to be one of the key components involved in the responses of tumor cells to chemotherapy. The aim of this study was to reveal the pathways and the phases of cisplatin-induced cell death that are characterized by changes of H 2 O 2 level. Methods: The genetically encoded cytosolic fluorescent sensor HyPer2 was used for flow cytometric analysis of the cisplatin-induced changes in H 2 O 2 level in HeLa Kyoto cells. Using a vital dye and the apoptotic markers PE Annexin V or TMRE the pathways and stages of cell death were investigated simultaneously with HyPer2 response. The H 2 O 2 level was studied separately in viable and early apoptotic cells after 12, 18, 24 h's incubation with cisplatin at several concentrations with or without the scavenger of reactive oxygen species NAC. Results: Cisplatin causes dose- and time-dependent increase of H 2 O 2 level in TMRE-positive and PE Annexin V-negative cancer cells. The scavenging of ROS by NAC
Ключевые слова:
Apoptosis; Cancer cell; Cisplatin; Flow cytometry; Genetically encoded sensor HyPer2; Hydrogen peroxide
cisplatin; hydrogen peroxide; lipocortin 5; phosphatidylserine; reactive oxygen metabolite; acetylcysteine; antineoplastic agent; cisplatin; hydrogen peroxide; antineoplastic activity; Article; cell death; cell fate; cell viability; cervical carcinoma cell line; concentration response; controlled study; human; human cell; IC50; mitochondrial membrane potential; priority journal; signal transduction; treatment response; apoptosis; cell proliferation; drug effect; drug screening; female; HeLa cell line; metabolism; pathology; uterine cervix tumor; Acetylcysteine; Antineoplastic Agents; Apoptosis; Cell Proliferation; Cisplatin; Drug Screening Assays, Antitumor; Female; HeLa Cells; Humans; Hydrogen Peroxide; Uterine Cervical Neoplasms
Язык текста: Английский
ISSN: 1872-8006
Nerush A. S.
Шсхукина К. М.
Balalaeva I. V. Irina Vladimirovna 1978-
Orlova A. G.
Неруш А. С.
Shshukina K. M.
Балалаева И. В. Ирина Владимировна 1978-
Орлова А. Г.
Hydrogen peroxide in the reactions of cancer cells to cisplatin
Текст визуальный непосредственный
Biochimica et Biophysica Acta (BBA)/General Subjects
Elsevier Science Publisher B.V.
Vol.1863, Issue4 P. 692-702
2019
Статья
Apoptosis Cancer cell Cisplatin Flow cytometry Genetically encoded sensor HyPer2 Hydrogen peroxide
cisplatin hydrogen peroxide lipocortin 5 phosphatidylserine reactive oxygen metabolite acetylcysteine antineoplastic agent cisplatin hydrogen peroxide antineoplastic activity Article cell death cell fate cell viability cervical carcinoma cell line concentration response controlled study human human cell IC50 mitochondrial membrane potential priority journal signal transduction treatment response apoptosis cell proliferation drug effect drug screening female HeLa cell line metabolism pathology uterine cervix tumor Acetylcysteine Antineoplastic Agents Apoptosis Cell Proliferation Cisplatin Drug Screening Assays, Antitumor Female HeLa Cells Humans Hydrogen Peroxide Uterine Cervical Neoplasms
Background: Hydrogen peroxide (H 2 O 2 ) is thought to be one of the key components involved in the responses of tumor cells to chemotherapy. The aim of this study was to reveal the pathways and the phases of cisplatin-induced cell death that are characterized by changes of H 2 O 2 level. Methods: The genetically encoded cytosolic fluorescent sensor HyPer2 was used for flow cytometric analysis of the cisplatin-induced changes in H 2 O 2 level in HeLa Kyoto cells. Using a vital dye and the apoptotic markers PE Annexin V or TMRE the pathways and stages of cell death were investigated simultaneously with HyPer2 response. The H 2 O 2 level was studied separately in viable and early apoptotic cells after 12, 18, 24 h's incubation with cisplatin at several concentrations with or without the scavenger of reactive oxygen species NAC. Results: Cisplatin causes dose- and time-dependent increase of H 2 O 2 level in TMRE-positive and PE Annexin V-negative cancer cells. The scavenging of ROS by NAC