Open-Source Multiparametric Optocardiography
Cathey B., Obaid S., Zolotarev A. M., Pryamonosov R. A., Syunyaev R. A., George S. A., Efimov I. R.
Scientific Reports
Vol.9, Issue1, Num.721
Опубликовано: 2019
Тип ресурса: Статья
DOI:10.1038/s41598-018-36809-y
Аннотация:
Since the 1970s fluorescence imaging has become a leading tool in the discovery of mechanisms of cardiac function and arrhythmias. Gradual improvements in fluorescent probes and multi-camera technology have increased the power of optical mapping and made a major impact on the field of cardiac electrophysiology. Tandem-lens optical mapping systems facilitated simultaneous recording of multiple parameters characterizing cardiac function. However, high cost and technological complexity restricted its proliferation to the wider biological community. We present here, an open-source solution for multiple-camera tandem-lens optical systems for multiparametric mapping of transmembrane potential, intracellular calcium dynamics and other parameters in intact mouse hearts and in rat heart slices. This 3D-printable hardware and Matlab-based RHYTHM 1.2 analysis software are distributed under an MIT open-source license. Rapid prototyping permits the development of inexpensive, customized systems wit
Ключевые слова:
calcium; fluorescent dye; animal; chemistry; devices; epicardium mapping; heart; metabolism; mouse; perfusion; physiology; procedures; rat; software; voltage sensitive dye imaging; Animals; Calcium; Epicardial Mapping; Fluorescent Dyes; Heart; Mice; Perfusion; Rats; Software; Voltage-Sensitive Dye Imaging
Язык текста: Английский
ISSN: 2045-2322
Cathey B.
Obaid S.
Zolotarev A. M.
Pryamonosov R. A. Roman Aleksandrovich 1992-
Syunyaev R. A. Roman Al`bertovich 1986-
George S. A.
Efimov I. R.
Cатхеy Б.
Обаид С.
Золотарев А. М.
Прямоносов Р. А. Роман Александрович 1992-
Сюняев Р. А. Роман Альбертович 1986-
Георге С. А.
Ефимов И. Р.
Open-Source Multiparametric Optocardiography
Текст визуальный непосредственный
Scientific Reports
Vol.9, Issue1 Num.721
2019
Статья
calcium fluorescent dye animal chemistry devices epicardium mapping heart metabolism mouse perfusion physiology procedures rat software voltage sensitive dye imaging Animals Calcium Epicardial Mapping Fluorescent Dyes Heart Mice Perfusion Rats Software Voltage-Sensitive Dye Imaging
Since the 1970s fluorescence imaging has become a leading tool in the discovery of mechanisms of cardiac function and arrhythmias. Gradual improvements in fluorescent probes and multi-camera technology have increased the power of optical mapping and made a major impact on the field of cardiac electrophysiology. Tandem-lens optical mapping systems facilitated simultaneous recording of multiple parameters characterizing cardiac function. However, high cost and technological complexity restricted its proliferation to the wider biological community. We present here, an open-source solution for multiple-camera tandem-lens optical systems for multiparametric mapping of transmembrane potential, intracellular calcium dynamics and other parameters in intact mouse hearts and in rat heart slices. This 3D-printable hardware and Matlab-based RHYTHM 1.2 analysis software are distributed under an MIT open-source license. Rapid prototyping permits the development of inexpensive, customized systems wit