Expression of TNFα receptors on immunocompetent cells is increased in atopic dermatitis
Lopatnikova J. A., Alshevskaya A. A., Krugleeva O. L., Nepomnyschih V. M., Gladkikh V. S., Lukinov V. L., Karaulov A. V., Sennikov S. V.
International Archives of Allergy and Immunology
Опубликовано: 2017
Тип ресурса: Статья
Аннотация:
Background: Expression levels of cytokine and growth factor receptors have been found to be important in the regulation of their action. Tumor necrosis factor-α (TNFα) is actively involved in inflammation processes in atopic dermatitis (AD), but the role of TNFα membrane receptors (TNFR) and their regulatory function in AD remains unclear. Aim: We aimed to determine the associations of parameters of TNFRα expression on immunocompetent cells with disease severity before and after therapy in AD patients. Methods: TNFRα expression on T cells, B cells, and monocytes was evaluated by flow cytometry. To determine receptor numbers on the cells, Quantibrite PE beads were used. The content of soluble mediators was evaluated by ELISA. To reveal linear relationships between the index scoring AD (SCORAD) and the studied parameters, multiple linear regression model building was used. Results: TNFR1 and TNFR2 expression in lymphocyte and monocyte populations of AD patients was higher than in healthy
Ключевые слова:
Atopic dermatitis; Membrane-bound receptors; Receptor expression density; Tumor necrosis factor
betamethasone; cetirizine; dexamethasone; emollient agent; tumor necrosis factor receptor 1; tumor necrosis factor receptor 2; tumor necrosis factor receptor; adult; Article; atopic dermatitis; B lymphocyte; cell population; clinical article; controlled study; disease severity; enzyme linked immunosorbent assay; female; flow cytometry; human; human cell; immunocompetent cell; male; monocyte; peripheral blood mononuclear cell; priority journal; receptor density; scorad index; scoring system; steroid therapy; T lymphocyte; atopic dermatitis; cell separation; disease exacerbation; genetics; immunocompetence; immunology; metabolism; middle aged; severity of illness index; upregulation; young adult; Adult; B-Lymphocytes; Cell Separation; Dermatitis, Atopic; Disease Progression; Female; Flow Cytometry; Humans; Immunocompetence; Male; Middle Aged; Monocytes; Receptors, Tumor Necrosis Factor; Severity of Illness Index; T-Lymphocytes; Up-Regulation; Young Adult
Язык текста: Английский
ISSN: 1423-0097
Lopatnikova J. A.
Alshevskaya A. A.
Krugleeva O. L.
Nepomnyschih V. M.
Gladkikh V. S.
Lukinov V. L.
Karaulov A. V. Aleksandr Viktorovich 1953-
Sennikov S. V.
Лопатникова Й. А.
Алшевскайа А. А.
Круглеева О. Л.
Непомнyсчих В. М.
Гладких В. С.
Лукинов В. Л.
Караулов А. В. Александр Викторович 1953-
Сенников С. В.
Expression of TNFα receptors on immunocompetent cells is increased in atopic dermatitis
Текст визуальный непосредственный
International Archives of Allergy and Immunology
S. Karger AG
Vol.174, Issue3-4
2017
Статья
Atopic dermatitis Membrane-bound receptors Receptor expression density Tumor necrosis factor
betamethasone cetirizine dexamethasone emollient agent tumor necrosis factor receptor 1 tumor necrosis factor receptor 2 tumor necrosis factor receptor adult Article atopic dermatitis B lymphocyte cell population clinical article controlled study disease severity enzyme linked immunosorbent assay female flow cytometry human human cell immunocompetent cell male monocyte peripheral blood mononuclear cell priority journal receptor density scorad index scoring system steroid therapy T lymphocyte atopic dermatitis cell separation disease exacerbation genetics immunocompetence immunology metabolism middle aged severity of illness index upregulation young adult Adult B-Lymphocytes Cell Separation Dermatitis, Atopic Disease Progression Female Flow Cytometry Humans Immunocompetence Male Middle Aged Monocytes Receptors, Tumor Necrosis Factor Severity of Illness Index T-Lymphocytes Up-Regulation Young Adult
Background: Expression levels of cytokine and growth factor receptors have been found to be important in the regulation of their action. Tumor necrosis factor-α (TNFα) is actively involved in inflammation processes in atopic dermatitis (AD), but the role of TNFα membrane receptors (TNFR) and their regulatory function in AD remains unclear. Aim: We aimed to determine the associations of parameters of TNFRα expression on immunocompetent cells with disease severity before and after therapy in AD patients. Methods: TNFRα expression on T cells, B cells, and monocytes was evaluated by flow cytometry. To determine receptor numbers on the cells, Quantibrite PE beads were used. The content of soluble mediators was evaluated by ELISA. To reveal linear relationships between the index scoring AD (SCORAD) and the studied parameters, multiple linear regression model building was used. Results: TNFR1 and TNFR2 expression in lymphocyte and monocyte populations of AD patients was higher than in healthy