Characterization of an archaeal photoreceptor/transducer complex from: Natronomonas pharaonis assembled within styrene-maleic acid lipid particles
Voskoboynikova N., Mosslehy W., Colbasevici A., Ismagulova T. T., Bagrov D. V., Akovantseva A. A., Timashev P. S., Mulkidjanian A. Y., Bagratashvili V. N., Shaitan K. V., Kirpichnikov M. P., Steinhoff H. -.
RSC Advances
Vol.7, Issue81, P. 51324-51334
Опубликовано: 2017
Тип ресурса: Статья
Аннотация:
The styrene-maleic acid (SMA) copolymers enable a detergent-free extraction of membrane proteins from lipid bilayers yielding stable water-soluble nanocontainers with lipid-encased proteins. Here, we demonstrate the ability of the SMA copolymer to extract a complex of interacting membrane proteins from proteoliposomes, namely the archaeal sensory rhodopsin II from Natronomonas pharaonis, NpSRII, in complex with its transducer, NpHtrII. The NpSRII/NpHtrII complexes retained their structural integrity within SMA lipid particles as revealed by diverse biophysical techniques. Pulse electron paramagnetic resonance data showed the SMA-encased NpSRII/NpHtrII complexes as native-like 2:2 dimers with a distance between NpSRII sites 159 according to the "V"-shaped conformation found in a crystal structure. © 2017 The Royal Society of Chemistry.
Ключевые слова:
Biological membranes; Crystal structure; Lipid bilayers; Magnetic resonance; Paramagnetic resonance; Styrene; Biophysical techniques; Lipid particles; Maleic acids; Membrane proteins; Nanocontainers; Proteoliposomes; Watersoluble; Yielding stable; Proteins
Язык текста: Английский
ISSN: 2046-2069
Voskoboynikova N.
Mosslehy W.
Colbasevici A.
Ismagulova T. T.
Bagrov D. V.
Akovantseva A. A. Anastasiya Aleksandrovna 1988-
Timashev P. S. Petr Sergeevich 1978-
Mulkidjanian A. Y.
Bagratashvili V. N.
Shaitan K. V.
Kirpichnikov M. P.
Steinhoff H. -. H.-J.
Воскобоyникова Н.
Мосслехy W.
Cолбасевиcи А.
Исмагулова Т. Т.
Багров Д. В.
Акованцева А. А. Анастасия Александровна 1988-
Тимашев П. С. Петр Сергеевич 1978-
Мулкидйаниан А. Y.
Баграташвили В. Н.
Шаитан К. В.
Кирпичников М. П.
Стеинхофф Х. -. Х.-Й.
Characterization of an archaeal photoreceptor/transducer complex from: Natronomonas pharaonis assembled within styrene-maleic acid lipid particles
Текст визуальный непосредственный
RSC Advances
Vol.7, Issue81 P. 51324-51334
2017
Статья
Biological membranes Crystal structure Lipid bilayers Magnetic resonance Paramagnetic resonance Styrene Biophysical techniques Lipid particles Maleic acids Membrane proteins Nanocontainers Proteoliposomes Watersoluble Yielding stable Proteins
The styrene-maleic acid (SMA) copolymers enable a detergent-free extraction of membrane proteins from lipid bilayers yielding stable water-soluble nanocontainers with lipid-encased proteins. Here, we demonstrate the ability of the SMA copolymer to extract a complex of interacting membrane proteins from proteoliposomes, namely the archaeal sensory rhodopsin II from Natronomonas pharaonis, NpSRII, in complex with its transducer, NpHtrII. The NpSRII/NpHtrII complexes retained their structural integrity within SMA lipid particles as revealed by diverse biophysical techniques. Pulse electron paramagnetic resonance data showed the SMA-encased NpSRII/NpHtrII complexes as native-like 2:2 dimers with a distance between NpSRII sites 159 according to the "V"-shaped conformation found in a crystal structure. © 2017 The Royal Society of Chemistry.