Development of Bright and Biocompatible Nanoruby and Its Application to Background-Free Time-Gated Imaging of G-Protein-Coupled Receptors
Sreenivasan V. K. A., Wan R. W. A., Zhang K., Pillai R. R., Saini A., Denkova D., Santiago M., Brown H., Thompson J., Connor M., Goldys E. M., Zvyagin A. V.
ACS Applied Materials & Interfaces
Vol.9, Issue45, P. 39197-39208
Опубликовано: 2017
Тип ресурса: Статья
DOI:10.1021/acsami.7b12665
Аннотация:
At the forefront of developing fluorescent probes for biological imaging applications are enhancements aimed at increasing their brightness, contrast, and photostability, especially toward demanding applications of single-molecule detection. In comparison with existing probes, nanorubies exhibit unlimited photostability and a long emission lifetime (∼4 ms), which enable continuous imaging at single-particle sensitivity in highly scattering and fluorescent biological specimens. However, their wide application as fluorescence probes has so far been hindered by the absence of facile methods for scaled-up high-volume production and molecularly specific targeting. The present work encompasses the large-scale production of colloidally stable nanoruby particles, the demonstration of their biofunctionality and negligible cytotoxicity, as well as the validation of its use for targeted biomolecular imaging. In addition, optical characteristics of nanorubies are found to be comparable or superior
Ключевые слова:
GPCR; nanoruby; opioid; single-particle; time-gated microscopy
Biocompatibility; Fluorescence; Probes; Quantum theory; Semiconductor quantum dots; G protein coupled receptors; GPCR; Large scale productions; nanoruby; opioid; Optical characteristics; Single particle; Single-molecule detection; Proteins; biomaterial; fluorescent dye; G protein coupled receptor; nanomaterial; quantum dot; time factor; Biocompatible Materials; Fluorescent Dyes; Nanostructures; Quantum Dots; Receptors, G-Protein-Coupled; Time Factors
Язык текста: Английский
ISSN: 1944-8252
Sreenivasan V. K. A.
Wan R. W. A. Razali W.A.
Zhang K.
Pillai R. R.
Saini A.
Denkova D.
Santiago M.
Brown H.
Thompson J.
Connor M.
Goldys E. M.
Zvyagin A. V. Andrej Vasilyevich 1961-
Среенивасан В. К. А.
Wан Р. W. А. Разали W.А.
Жанг К.
Пиллаи Р. Р.
Саини А.
Денкова Д.
Сантиаго М.
Броwн Х.
Тхомпсон Й.
Cоннор М.
Голдyс Е. М.
Звягин А. В. Андрей Васильевич 1961-
Development of Bright and Biocompatible Nanoruby and Its Application to Background-Free Time-Gated Imaging of G-Protein-Coupled Receptors
Текст визуальный непосредственный
ACS Applied Materials & Interfaces
American Chemical Society
Vol.9, Issue45 P. 39197-39208
2017
Статья
GPCR nanoruby opioid single-particle time-gated microscopy
Biocompatibility Fluorescence Probes Quantum theory Semiconductor quantum dots G protein coupled receptors GPCR Large scale productions nanoruby opioid Optical characteristics Single particle Single-molecule detection Proteins biomaterial fluorescent dye G protein coupled receptor nanomaterial quantum dot time factor Biocompatible Materials Fluorescent Dyes Nanostructures Quantum Dots Receptors, G-Protein-Coupled Time Factors
At the forefront of developing fluorescent probes for biological imaging applications are enhancements aimed at increasing their brightness, contrast, and photostability, especially toward demanding applications of single-molecule detection. In comparison with existing probes, nanorubies exhibit unlimited photostability and a long emission lifetime (∼4 ms), which enable continuous imaging at single-particle sensitivity in highly scattering and fluorescent biological specimens. However, their wide application as fluorescence probes has so far been hindered by the absence of facile methods for scaled-up high-volume production and molecularly specific targeting. The present work encompasses the large-scale production of colloidally stable nanoruby particles, the demonstration of their biofunctionality and negligible cytotoxicity, as well as the validation of its use for targeted biomolecular imaging. In addition, optical characteristics of nanorubies are found to be comparable or superior