Effect of Chronic Alcohol Abuse on Anabolic and Catabolic Signaling Pathways in Human Skeletal Muscle
Shenkman B. S., Belova S. P., Zinovyeva O. E., Samkhaeva N. D., Mirzoev T. M., Vilchinskaya N. A., Altaeva E. G., Turtikova O. V., Kostrominova T. Y., Nemirovskaya T. L.
Alcoholism: Clinical and Experimental Research
Vol.42, Issue1, P. 41-52
Опубликовано: 2018
Тип ресурса: Статья
Аннотация:
Background: Animal studies showed that alcoholic myopathy is characterized by the reduction in myofiber cross-sectional area (CSA) and by impaired anabolic signaling. The goal of this study was to compare changes in CSA and fiber type composition with modifications in anabolic and catabolic signaling pathways at the early stages of alcohol misuse in humans. Methods: Skeletal muscle samples from 7 male patients with chronic alcohol abuse (AL; 47.7 ± 2.0 years old; alcohol misuse duration 7.7 ± 0.6 years) were compared with muscle from a control group of 7 healthy men (C; 39.7 ± 5.0 years old). Biopsies from vastus lateralis muscles were taken and analyzed for the changes in fiber type composition, fiber CSA, and for the alterations in anabolic and catabolic signaling pathways. Results: AL patients did not have detectable clinical myopathy symptoms or muscle fiber atrophy, but the relative proportion of fast fibers was increased. There was a significant decrease in IGF-1 in plasma and IR
Ключевые слова:
Ethanol; IGF-1; IRS-1; p70S6K1; p90RSK1; Ubiquitinated Proteins
alcohol; heat shock protein 70; heat shock protein 90; insulin receptor substrate 1; messenger RNA; somatomedin C; reactive oxygen metabolite; adult; alcohol abuse; Article; biosynthesis; catabolism; controlled study; human; human tissue; male; middle aged; muscle atrophy; muscle biopsy; myopathy; priority journal; protein blood level; protein content; protein expression; signal transduction; skeletal muscle; vastus lateralis muscle; alcoholism; chemically induced; complication; drug effect; metabolism; pathology; physiology; signal transduction; skeletal muscle; Adult; Alcoholism; Humans; Male; Metabolism; Middle Aged; Muscle, Skeletal; Muscular Atrophy; Reactive Oxygen Species; Signal Transduction
Язык текста: Английский
ISSN: 1530-0277
Shenkman B. S.
Belova S. P.
Zinovyeva O. E. Ol`ga Evgenyevna 1963-
Samkhaeva N. D. Nyudlya Dordzhievna 1986-
Mirzoev T. M.
Vilchinskaya N. A.
Altaeva E. G.
Turtikova O. V.
Kostrominova T. Y.
Nemirovskaya T. L.
Шенкман Б. С.
Белова С. П.
Зиновьева О. Е. Ольга Евгеньевна 1963-
Самхаева Н. Д. Нюдля Дорджиевна 1986-
Мирзоев Т. М.
Вилчинскайа Н. А.
Алтаева Е. Г.
Тюртикова О. В.
Костроминова Т. Y.
Немировскайа Т. Л.
Effect of Chronic Alcohol Abuse on Anabolic and Catabolic Signaling Pathways in Human Skeletal Muscle
Текст визуальный непосредственный
Alcoholism: Clinical and Experimental Research
Lippincott Williams & Wilkins
Vol.42, Issue1 P. 41-52
2018
Статья
Ethanol IGF-1 IRS-1 p70S6K1 p90RSK1 Ubiquitinated Proteins
alcohol heat shock protein 70 heat shock protein 90 insulin receptor substrate 1 messenger RNA somatomedin C reactive oxygen metabolite adult alcohol abuse Article biosynthesis catabolism controlled study human human tissue male middle aged muscle atrophy muscle biopsy myopathy priority journal protein blood level protein content protein expression signal transduction skeletal muscle vastus lateralis muscle alcoholism chemically induced complication drug effect metabolism pathology physiology signal transduction skeletal muscle Adult Alcoholism Humans Male Metabolism Middle Aged Muscle, Skeletal Muscular Atrophy Reactive Oxygen Species Signal Transduction
Background: Animal studies showed that alcoholic myopathy is characterized by the reduction in myofiber cross-sectional area (CSA) and by impaired anabolic signaling. The goal of this study was to compare changes in CSA and fiber type composition with modifications in anabolic and catabolic signaling pathways at the early stages of alcohol misuse in humans. Methods: Skeletal muscle samples from 7 male patients with chronic alcohol abuse (AL; 47.7 ± 2.0 years old; alcohol misuse duration 7.7 ± 0.6 years) were compared with muscle from a control group of 7 healthy men (C; 39.7 ± 5.0 years old). Biopsies from vastus lateralis muscles were taken and analyzed for the changes in fiber type composition, fiber CSA, and for the alterations in anabolic and catabolic signaling pathways. Results: AL patients did not have detectable clinical myopathy symptoms or muscle fiber atrophy, but the relative proportion of fast fibers was increased. There was a significant decrease in IGF-1 in plasma and IR