The brain after ischemic stroke: A clinical/histological study
Сергеева С. П., Савин А. А., Шишкина Л. В., Виноградов Е. В.
Журнал неврологии и психиатрии им. C.C. Корсакова
Т. 117, Вып. 3, С. 66-70
Опубликовано: 2017
Тип ресурса: Статья
DOI:10.17116/jnevro20171173266-70
Аннотация:
Objective. To analyze the histological features of changes in the brain tissue after ischemic stroke. Material and methods. Brain tissue samples obtained in autopsy from 9 people died as a result of a left middle cerebral artery ischemic stroke from 3 to 7 days after admission were studied. Tissue samples were taken from 3 areas of the brain: 1) contiguous to the tissue necrotic damage site zone, 2) 5—10 cm distant from the previous one, 3) the contralateral hemisphere zone symmetrical to damage site. Samples were Nissl and hematoxylin-eosin stained. Detection of p53 protein, NSE, GFAP was performed by indirect immunoperoxidase immunohistochemistry. Results. A decrease in the total number of neurons and glial elements, their spatial redistribution, change in cell structure and their functional activity was found. The changes of the artery wall and impaired regional blood flow were detected. The more intense NSE reactivity; p53-positive neurons, reduced neuron-astrocytes distance were i
Ключевые слова:
Brain; Histological study; Immunohistochemical study; Ischemic stroke; р53-positive neurons
peroxidase; artery wall; Article; astrocyte; autopsy; brain ischemia; brain tissue; cell structure; clinical study; histology; human; human tissue; immunohistochemistry; middle cerebral artery; protein analysis; tissue injury; tissue necrosis; brain; cerebrovascular accident; complication; nerve cell; pathology; Astrocytes; Brain; Brain Ischemia; Humans; Neurons; Stroke
Язык текста: Русский
ISSN: 2309-4729
Сергеева С. П.
Савин А. А. Андрей Александрович 1992-
Шишкина Л. В.
Виноградов Е. В.
Sergeeva S. P.
Savin A. A. Andrej Aleksandrovich 1992-
Shishkina L. V.
Vinogradov E. V.
The brain after ischemic stroke: A clinical/histological study
Текст визуальный непосредственный
Журнал неврологии и психиатрии им. C.C. Корсакова
Издательство Медиа Сфера
Т. 117, Вып. 3 С. 66-70
2017
Статья
Brain Histological study Immunohistochemical study Ischemic stroke р53-positive neurons
peroxidase artery wall Article astrocyte autopsy brain ischemia brain tissue cell structure clinical study histology human human tissue immunohistochemistry middle cerebral artery protein analysis tissue injury tissue necrosis brain cerebrovascular accident complication nerve cell pathology Astrocytes Brain Brain Ischemia Humans Neurons Stroke
Objective. To analyze the histological features of changes in the brain tissue after ischemic stroke. Material and methods. Brain tissue samples obtained in autopsy from 9 people died as a result of a left middle cerebral artery ischemic stroke from 3 to 7 days after admission were studied. Tissue samples were taken from 3 areas of the brain: 1) contiguous to the tissue necrotic damage site zone, 2) 5—10 cm distant from the previous one, 3) the contralateral hemisphere zone symmetrical to damage site. Samples were Nissl and hematoxylin-eosin stained. Detection of p53 protein, NSE, GFAP was performed by indirect immunoperoxidase immunohistochemistry. Results. A decrease in the total number of neurons and glial elements, their spatial redistribution, change in cell structure and their functional activity was found. The changes of the artery wall and impaired regional blood flow were detected. The more intense NSE reactivity; p53-positive neurons, reduced neuron-astrocytes distance were i