Comparative analysis of the PCA3 gene expression in sediments and exosomes isolated from urine
Михайленко Д. С., Новиков А. А., Григор'ева М. В., Ефремов Г. Д., Сивков А. В., Сафронова Н. Y., Сорокин К. С., Земскова М. Y., Немцова М. В., Алексеев Б. Y., Каприн А. Д.
Онкоурология
Т. 13, Вып. 3, С. 54-60
Опубликовано: 2017
Тип ресурса: Статья
DOI:10.17650/1726-9776-2017-13-3-54-60
Аннотация:
Introduction: Prostate cancer (PCa) is one of the common oncological diseases in men. Expression of the PCA3 gene in urine is currently used as a molecular genetic marker of PCa. Objective: to comparative analysis of the PCA3 expression in urine sediments and exosomes for the determination of the biomaterial, which allows detecting the PCA3 expression in more efficient manner. Materials and methods: The 12 patients with different stages of PCa and 8 control samples were examined. Results. The diagnostic accuracy of the PCA3 gene expression analysis in this cohort exceeded 90 [%]. We had not obtained significant differences in the sensitivity and specificity of the PCA3 hyperexpression in the urine sediments compared with exosomes. This result indicates in favor to using urine sediment for the PCA3 analysis as a biomaterial with less time-consuming sample preparation, although the possible advantage of exosomes for the analysis of the expression marker panels requires further studies.
Ключевые слова:
Exosome; Gene expression; Non-invasive diagnostics; PCA3
Язык текста: Русский
ISSN: 1996-1812
Михайленко Д. С. Дмитрий Сергеевич 1981-
Новиков А. А.
Григор'ева М. В.
Ефремов Г. Д.
Сивков А. В.
Сафронова Н. Y. Н.Ю.
Сорокин К. С.
Земскова М. Y. М.Ю.
Немцова М. В. Марина Вячеславовна 1962-
Алексеев Б. Y. Б.Я.
Каприн А. Д.
Mikhaylenko D. S. Dmitrij Sergeevich 1981-
Novikov A. A.
Grigor'eva M. V.
Efremov G. D.
Sivkov A. V.
Safronova N. Y. N.Yu.
Sorokin K. S.
Zemskova M. Y. M.Yu.
Nemtsova M. V. Marina Vyacheslavovna 1962-
Alekseev B. Y. B.Ya.
Kaprin A. D.
Comparative analysis of the PCA3 gene expression in sediments and exosomes isolated from urine
Текст визуальный непосредственный
Онкоурология
Издательский дом "АБВ-пресс"
Т. 13, Вып. 3 С. 54-60
2017
Статья
Exosome Gene expression Non-invasive diagnostics PCA3
Introduction: Prostate cancer (PCa) is one of the common oncological diseases in men. Expression of the PCA3 gene in urine is currently used as a molecular genetic marker of PCa. Objective: to comparative analysis of the PCA3 expression in urine sediments and exosomes for the determination of the biomaterial, which allows detecting the PCA3 expression in more efficient manner. Materials and methods: The 12 patients with different stages of PCa and 8 control samples were examined. Results. The diagnostic accuracy of the PCA3 gene expression analysis in this cohort exceeded 90 [%]. We had not obtained significant differences in the sensitivity and specificity of the PCA3 hyperexpression in the urine sediments compared with exosomes. This result indicates in favor to using urine sediment for the PCA3 analysis as a biomaterial with less time-consuming sample preparation, although the possible advantage of exosomes for the analysis of the expression marker panels requires further studies.