Effects of selective phosphodiesterase-2 and phosphodiesterase-3 inhibition on coronary flow and oxidative stress in the isolated rat heart
Stevanovic J., Zivkovic V., Bolevich S., Zaric G., Cekerevac I., Djuric D., Yakovlevich V., Nikolic T.
ACTA POLONIAE PHARMACEUTICA
Vol.74, Issue6, P. 1691-1698
Опубликовано: 2017
Тип ресурса: Статья
Аннотация:
The aim of the study was to investigate the effect of PDE2 (erythro-9-(2-hydroxy-3-nonyl) adenine (EHNA)) and PDE3 (Milrinon) inhibitors on coronary flow and oxidative stress markers in the isolated rat heart. Excised hearts of male Wistar albino rats (total of 12 for each experimental group) aged 8 weeks, body mass 180-200 g were retrogradely perfused according to Langendorff technique with gradually increased coronary perfusion pressure (CPP = 40-120 cm H2O). Samples of coronary venous effluent were collected after the stabilization of CF at each value of gradually increased perfusion pressure. Spectrophotometric method was used for determination of nitrite, super-oxide anion radical, and index of lipid peroxidation. Based on the results of this study it can be concluded that the inhibition of PDE3 system (in comparison to PDE2) can be more significant in the control of coronary vascular tonus, which may have potential clinical significance in the conditions of decreased coronary per
Ключевые слова:
Isolated rat heart; Milrinon; Oxidative stress; Phosphodiesterase
erythro 9 (2 hydroxy 3 nonyl) adenine; glutathione reductase; hydrogen peroxide; milrinone; nitrate; nitrite; phosphodiesterase inhibitor; superoxide; thiobarbituric acid reactive substance; unclassified drug; animal experiment; Article; blood vessel tone; cardiovascular autoregulation; controlled study; coronary artery; coronary artery blood flow; drug effect; isolated heart; lipid peroxidation; male; nonhuman; oxidative stress; rat; spectrophotometry
Язык текста: Английский
ISSN: 0001-6837
Stevanovic J.
Zivkovic V.
Bolevich S. Sergej 1964-
Zaric G.
Cekerevac I.
Djuric D.
Yakovlevich V. Vladimir 1971-
Nikolic T.
Djuric D.
Стевановиc Й.
Зивковиc В.
Болевич С. Сергей 1964-
Зариc Г.
Cекереваc И.
Дйуриc Д.
Яковлевич В. Владимир 1971-
Николиc Т.
Дйуриc Д.
Effects of selective phosphodiesterase-2 and phosphodiesterase-3 inhibition on coronary flow and oxidative stress in the isolated rat heart
Текст визуальный непосредственный
ACTA POLONIAE PHARMACEUTICA
Polskie Towarzystwo Farmaceutyczne/Polish Pharmaceutical Society
Vol.74, Issue6 P. 1691-1698
2017
Статья
Isolated rat heart Milrinon Oxidative stress Phosphodiesterase
erythro 9 (2 hydroxy 3 nonyl) adenine glutathione reductase hydrogen peroxide milrinone nitrate nitrite phosphodiesterase inhibitor superoxide thiobarbituric acid reactive substance unclassified drug animal experiment Article blood vessel tone cardiovascular autoregulation controlled study coronary artery coronary artery blood flow drug effect isolated heart lipid peroxidation male nonhuman oxidative stress rat spectrophotometry
The aim of the study was to investigate the effect of PDE2 (erythro-9-(2-hydroxy-3-nonyl) adenine (EHNA)) and PDE3 (Milrinon) inhibitors on coronary flow and oxidative stress markers in the isolated rat heart. Excised hearts of male Wistar albino rats (total of 12 for each experimental group) aged 8 weeks, body mass 180-200 g were retrogradely perfused according to Langendorff technique with gradually increased coronary perfusion pressure (CPP = 40-120 cm H2O). Samples of coronary venous effluent were collected after the stabilization of CF at each value of gradually increased perfusion pressure. Spectrophotometric method was used for determination of nitrite, super-oxide anion radical, and index of lipid peroxidation. Based on the results of this study it can be concluded that the inhibition of PDE3 system (in comparison to PDE2) can be more significant in the control of coronary vascular tonus, which may have potential clinical significance in the conditions of decreased coronary per