Identification of Shiga toxin in patients with acute intestinal infections in the presence of monoand mixed-O-antigens of pathogens
Белая О. Ф., Юдина Y. В., Волчкова Е. В., Пайевскайа О. А., Белайа Y. А., Гюлазян Н. М., Зуьевскайа С. Н.
Терапевтический архив
Т. 89, Вып. 11, С. 55-59
Опубликовано: 2017
Тип ресурса: Статья
DOI:10.17116/terarkh2017891155-59
Аннотация:
Aim. To investigate the time course of changes in the detection rates and levels of Shiga toxin antigen (STA) in their stool and middle-molecule circulating immune complexes (CICs) containing IgG (IgG CIC) in patients with acute intestinal infections (AIIs) in the presence of the body's circulation of mono- and mixed-LPS/O-antigens of intestinal pathogens. Subjects and methods. A total of 147 patients aged 15 to 55 years who had been hospitalized with AIIs were examined. The diagnosis was bacteriologically verified in 19[%] of the patients; in the others, it was confirmed by the detection of LPS/O-antigens of Shigella, Salmonella, Yersinia, and Campylobacter in their stool by means of the reaction of coagglutination (RCA) on glass slides. Plates for RCA displayed STA in the fecal and IgG CIC samples. Results. Mono- and mixed infections were detected in 32 and 68[%], respectively. The RCA plates exhibited STA in 25.2[%] of the fecal samples and in 90.5[%] of the IgG CIC ones from patients with
Ключевые слова:
Antigen; Immune complex; Intestinal infection; Lipopolysaccharide; Reaction of coagglutination; Shiga toxin
O antigen; Shiga toxin; acute disease; adolescent; adult; comorbidity; Enterobacteriaceae infection; enteropathy; female; human; immunology; male; microbiological examination; middle aged; mixed infection; procedures; standards; young adult; Acute Disease; Adolescent; Adult; Bacteriological Techniques; Coinfection; Comorbidity; Enterobacteriaceae Infections; Female; Humans; Intestinal Diseases; Male; Middle Aged; O Antigens; Shiga Toxin; Young Adult
Язык текста: Русский
ISSN: 2309-5342
Белая О. Ф. Ольга Федоровна 1953-
Юдина Y. В. Ю.В.
Волчкова Е. В. Елена Васильевна 1953-
Пайевскайа О. А.
Белайа Y. А. Ю.А.
Гюлазян Н. М.
Зуьевскайа С. Н.
Belaya O. F. Ol`ga Fedorovna 1953-
Yudina Y. V. Yu.V.
Volchkova E. V. Elena Vasilyevna 1953-
Payevskaya O. A.
Belaya Y. A. Yu.A.
Gyulazyan N. M.
Zuyevskaya S. N.
Identification of Shiga toxin in patients with acute intestinal infections in the presence of monoand mixed-O-antigens of pathogens
Текст визуальный непосредственный
Терапевтический архив
Медицинское маркетинговое агентство "МедиаМедика"
Т. 89, Вып. 11 С. 55-59
2017
Статья
Antigen Immune complex Intestinal infection Lipopolysaccharide Reaction of coagglutination Shiga toxin
O antigen Shiga toxin acute disease adolescent adult comorbidity Enterobacteriaceae infection enteropathy female human immunology male microbiological examination middle aged mixed infection procedures standards young adult Acute Disease Adolescent Adult Bacteriological Techniques Coinfection Comorbidity Enterobacteriaceae Infections Female Humans Intestinal Diseases Male Middle Aged O Antigens Shiga Toxin Young Adult
Aim. To investigate the time course of changes in the detection rates and levels of Shiga toxin antigen (STA) in their stool and middle-molecule circulating immune complexes (CICs) containing IgG (IgG CIC) in patients with acute intestinal infections (AIIs) in the presence of the body's circulation of mono- and mixed-LPS/O-antigens of intestinal pathogens. Subjects and methods. A total of 147 patients aged 15 to 55 years who had been hospitalized with AIIs were examined. The diagnosis was bacteriologically verified in 19[%] of the patients; in the others, it was confirmed by the detection of LPS/O-antigens of Shigella, Salmonella, Yersinia, and Campylobacter in their stool by means of the reaction of coagglutination (RCA) on glass slides. Plates for RCA displayed STA in the fecal and IgG CIC samples. Results. Mono- and mixed infections were detected in 32 and 68[%], respectively. The RCA plates exhibited STA in 25.2[%] of the fecal samples and in 90.5[%] of the IgG CIC ones from patients with