Inhibitory effect of streptococci on the growth of M. catarrhalis strains and the diversity of putative bacteriocin-like gene loci in the genomes...
Ikryannikova L. N., Malakhova M. V., Lominadze G. G., Karpova I. Y., Kostryukova E. S., Mayanskij N. A., Kruglov A. N., Klimova E. A., Lisitsina E. S., Ilina E. N., Govorun V. M.
AMB Express
Vol.7, Issue1, Num.218
Опубликовано: 2017
Тип ресурса: Статья
DOI:10.1186/s13568-017-0521-z
Аннотация:
S. pneumoniae is a facultative human pathogen causing a wide range of infections including the life-threatening pneumoniae or meningitis. It colonizes nasopharynx as well as its closest phylogenetic relatives S. pseudopneumoniae and S. mitis. Both the latter, despite the considerable morphological and phenotypic similarity with the pneumococcus, are considerably less pathogenic for humans and cause infections mainly in the immunocompromized hosts. In this work, we compared the inhibitory effect of S. pneumoniae and its relatives on the growth of Moraxella catarrhalis strains using the culture-based antagonistic test. We observed that the inhibitory effect of S. mitis strains is kept when a hydrogen peroxide produced by cells is inactivated by catalase, and even when the live cells are killed in chloroform vapors, in contrast to the pneumococcus whose inhibiting ability disappeared when the cells die. It was suggested that this effect may be due to the production of bacterial antimicrob
Ключевые слова:
Bacteriocin-associated gene loci; M. catarrhalis growth inhibition; S. pneumoniae and its relatives; Viridans group streptococci
bacteriocin; Article; DNA extraction; gene cluster; gene locus; genetic manipulation; Klebsiella pneumoniae; latex agglutination test; Moraxella catarrhalis; multilocus sequence typing; nonhuman; Streptococcus; whole genome sequencing
Язык текста: Английский
ISSN: 2191-0855
Ikryannikova L. N. Larisa Nikolaevna 1974-
Malakhova M. V.
Lominadze G. G.
Karpova I. Y.
Kostryukova E. S.
Mayanskij N. A. Nikolay Andreevich 1972-
Kruglov A. N.
Klimova E. A.
Lisitsina E. S.
Ilina E. N.
Govorun V. M.
Икрянникова Л. Н. Лариса Николаевна 1974-
Малахова М. В.
Ломинадзе Г. Г.
Карпова И. Y.
Кострюкова Е. С.
Маянский Н. А. Николай Андреевич 1972-
Круглов А. Н.
Климова Е. А.
Лисицина Е. С.
Илина Е. Н.
Говорун В. М.
Inhibitory effect of streptococci on the growth of M. catarrhalis strains and the diversity of putative bacteriocin-like gene loci in the genomes of S. pneumoniae and its relatives
Inhibitory effect of streptococci on the growth of M. catarrhalis strains and the diversity of putative bacteriocin-like gene loci in the genomes...
Текст визуальный непосредственный
AMB Express
Vol.7, Issue1 Num.218
2017
Статья
Bacteriocin-associated gene loci M. catarrhalis growth inhibition S. pneumoniae and its relatives Viridans group streptococci
bacteriocin Article DNA extraction gene cluster gene locus genetic manipulation Klebsiella pneumoniae latex agglutination test Moraxella catarrhalis multilocus sequence typing nonhuman Streptococcus whole genome sequencing
S. pneumoniae is a facultative human pathogen causing a wide range of infections including the life-threatening pneumoniae or meningitis. It colonizes nasopharynx as well as its closest phylogenetic relatives S. pseudopneumoniae and S. mitis. Both the latter, despite the considerable morphological and phenotypic similarity with the pneumococcus, are considerably less pathogenic for humans and cause infections mainly in the immunocompromized hosts. In this work, we compared the inhibitory effect of S. pneumoniae and its relatives on the growth of Moraxella catarrhalis strains using the culture-based antagonistic test. We observed that the inhibitory effect of S. mitis strains is kept when a hydrogen peroxide produced by cells is inactivated by catalase, and even when the live cells are killed in chloroform vapors, in contrast to the pneumococcus whose inhibiting ability disappeared when the cells die. It was suggested that this effect may be due to the production of bacterial antimicrob