Approaches of the transcriptomic analysis in astrocytes: Potential pharmacological targets
Barreto G. E., Gómez R. M., Bustos R. H., Forero D. A., Aliev G., Tarasov V. V., Yarla N. S., Echeverria V., Gonzalez J.
Current Pharmaceutical Design
Vol.23, Issue28, P. 4189-4197
Опубликовано: 2017
Тип ресурса: Обзор
DOI:10.2174/1381612823666170406113501
Аннотация:
Astrocytes are important glial cells involved in the ionic regulation of the extracellular fluid in the Central Nervous System (CNS), the formation of the blood brain barrier (BBB) and the support to neurons for the maintenance of the Krebs cycle intermediaries. Even though these cells are known to be important for the brain functioning, several of their functions and their development have not been fully elucidated. In this context, identifying the algorithms used for their analysis plays a pivotal role in the development of new strategies in the study of astrocytes. The main objective of this review is to summarize the techniques that have helped to obtain transcriptomic data in astrocytes and the new algorithms that were used to perform the analysis of experimental data, elucidating new studies in which these had been used. We also highlight the current transcriptomics approaches targeting astrocytes function as a possible target for pharmacological interventions. © 2017 Bentham Sci
Ключевые слова:
Astrocytes; Blood brain barrier; Brain; Central nervous system; Neurological disorders; Transcriptome methods
affymetrix array hybridization; astrocyte; blood brain barrier; brain injury; cell function; cell hybridization; central nervous system; chromosome comparative genomic hybridization; cluster analysis; data analysis; epilepsy; fluorescence in situ hybridization; gene expression; genetic algorithm; human; mental disease; neuroprotection; priority journal; Review; transcriptomics; algorithm; animal; astrocyte; brain; drug design; gene expression profiling; metabolism; nerve cell; procedures; transcriptome; Algorithms; Animals; Astrocytes; Blood-Brain Barrier; Brain; Central Nervous System; Drug Design; Gene Expression Profiling; Humans; Neurons; Transcriptome
Язык текста: Английский
ISSN: 1873-4286
Barreto G. E.
Gómez R. M.
Bustos R. H.
Forero D. A.
Aliev G. Gyumrakh 1958-
Tarasov V. V. Vadim Vladimirovich 1987-
Yarla N. S.
Echeverria V.
Gonzalez J.
Баррето Г. Е.
Гóмез Р. М.
Бустос Р. Х.
Фореро Д. А.
Алиев Г. Гюмрах 1958-
Тарасов В. В. Вадим Владимирович 1987-
Ярла Н. С.
Ечеверриа В.
Гонзалез Й.
Approaches of the transcriptomic analysis in astrocytes: Potential pharmacological targets
Текст визуальный непосредственный
Current Pharmaceutical Design
Bentham Science Publishers
Vol.23, Issue28 P. 4189-4197
2017
Обзор
Astrocytes Blood brain barrier Brain Central nervous system Neurological disorders Transcriptome methods
affymetrix array hybridization astrocyte blood brain barrier brain injury cell function cell hybridization central nervous system chromosome comparative genomic hybridization cluster analysis data analysis epilepsy fluorescence in situ hybridization gene expression genetic algorithm human mental disease neuroprotection priority journal Review transcriptomics algorithm animal astrocyte brain drug design gene expression profiling metabolism nerve cell procedures transcriptome Algorithms Animals Astrocytes Blood-Brain Barrier Brain Central Nervous System Drug Design Gene Expression Profiling Humans Neurons Transcriptome
Astrocytes are important glial cells involved in the ionic regulation of the extracellular fluid in the Central Nervous System (CNS), the formation of the blood brain barrier (BBB) and the support to neurons for the maintenance of the Krebs cycle intermediaries. Even though these cells are known to be important for the brain functioning, several of their functions and their development have not been fully elucidated. In this context, identifying the algorithms used for their analysis plays a pivotal role in the development of new strategies in the study of astrocytes. The main objective of this review is to summarize the techniques that have helped to obtain transcriptomic data in astrocytes and the new algorithms that were used to perform the analysis of experimental data, elucidating new studies in which these had been used. We also highlight the current transcriptomics approaches targeting astrocytes function as a possible target for pharmacological interventions. © 2017 Bentham Sci