Photodynamic therapy of mouse tumor model using chlorin e6- polyvinyl alcohol complex
Gavrina A. I., Shirmanova M. V., Aksenova N. A., Yuzhakova D. V., Snopova L. B., Solovieva A. B., Тимашев П. С., Dudenkova V. V., Zagaynova E. V.
Journal of Photochemistry and Photobiology B: Biology
Vol.178, P. 614-622
Опубликовано: 2018
Тип ресурса: Статья
DOI:10.1016/j.jphotobiol.2017.12.016
Аннотация:
The use of polymeric carriers to deliver hydrophobic photosensitizers has been widely discussed as a way to improve both fluorescence diagnostic and photodynamic therapy (PDT) of cancers; however, the photophysical and pharmacokinetic parameters, as well as the PDT activity, of such modifications have, until now, only been poorly investigated. The purpose of the present study was to explore the efficacy of PDT with the formulation of the photosensitizer chlorin e6 (Ce6) in combination with polyvinyl alcohol (PVA) in comparison with Ce6 alone and with the clinical drug, Photodithazine in a mouse tumor model. We also investigated the photoactivity of the Ce6-PVA in a model reaction of tryptophan oxidation, analyzed the polymer–Ce6 interaction using fluorescence spectroscopy and atomic-force microscopy, and tested the phototoxicity in vitro. Using fluorescence imaging in vivo we found that injection to mice of Ce6 in a formulation with PVA resulted in a higher tumor-to-normal ratio and gr
Ключевые слова:
Chlorin e6; Fluorescence imaging in vivo; Murine colon carcinoma; Photodynamic therapy; Photosensitizer; Polyvinyl alcohol
polyvinyl alcohol; talaporfin; tryptophan; photosensitizing agent; phytochlorin; polyvinyl alcohol; porphyrin; reactive oxygen metabolite; animal experiment; animal model; animal tissue; Article; atomic force microscopy; bioaccumulation; bleaching; cancer growth; cancer regression; chemical interaction; clinical effectiveness; colon carcinoma; controlled study; drug formulation; fluorescence spectroscopy; histopathology; in vitro study; molecular imaging; mouse; nanoencapsulation; nonhuman; oncological parameters; oxidation; photodynamic therapy; phototoxicity; priority journal; allotransplantation; animal; Bagg albino mouse; cell survival; chemistry; confocal microscopy; disease model; drug effects; neoplasm; oxidation reduction reaction; pathology; photochemotherapy; spectrofluorometry; tumor cell line; whole body imaging; Animals; Cell Line, Tumor; Cell Survival; Disease Models, Animal; Mice; Mice, Inbred BALB C; Microscopy, Atomic Force; Microscopy, Confocal; Neoplasms; Oxidation-R
Язык текста: Английский
ISSN: 1011-1344
Gavrina A. I.
Shirmanova M. V.
Aksenova N. A. Nadezhda Anatolyevna 1979-
Yuzhakova D. V.
Snopova L. B.
Solovieva A. B.
Тимашев П. С.
Dudenkova V. V.
Zagaynova E. V.
Гаврина А. И.
Ширманова М. В.
Аксенова Н. А. Надежда Анатольевна 1979-
Южакова Д. В.
Снопова Л. Б.
Соловиева А. Б.
Timashev P. S.
Дуденкова В. В.
Загайнова Е. В.
Photodynamic therapy of mouse tumor model using chlorin e6- polyvinyl alcohol complex
Текст визуальный непосредственный
Journal of Photochemistry and Photobiology B: Biology
Elsevier Science Publisher B.V.
Vol.178 P. 614-622
2018
Статья
Chlorin e6 Fluorescence imaging in vivo Murine colon carcinoma Photodynamic therapy Photosensitizer Polyvinyl alcohol
polyvinyl alcohol talaporfin tryptophan photosensitizing agent phytochlorin polyvinyl alcohol porphyrin reactive oxygen metabolite animal experiment animal model animal tissue Article atomic force microscopy bioaccumulation bleaching cancer growth cancer regression chemical interaction clinical effectiveness colon carcinoma controlled study drug formulation fluorescence spectroscopy histopathology in vitro study molecular imaging mouse nanoencapsulation nonhuman oncological parameters oxidation photodynamic therapy phototoxicity priority journal allotransplantation animal Bagg albino mouse cell survival chemistry confocal microscopy disease model drug effects neoplasm oxidation reduction reaction pathology photochemotherapy spectrofluorometry tumor cell line whole body imaging Animals Cell Line, Tumor Cell Survival Disease Models, Animal Mice Mice, Inbred BALB C Microscopy, Atomic Force Microscopy, Confocal Neoplasms Oxidation-R
The use of polymeric carriers to deliver hydrophobic photosensitizers has been widely discussed as a way to improve both fluorescence diagnostic and photodynamic therapy (PDT) of cancers; however, the photophysical and pharmacokinetic parameters, as well as the PDT activity, of such modifications have, until now, only been poorly investigated. The purpose of the present study was to explore the efficacy of PDT with the formulation of the photosensitizer chlorin e6 (Ce6) in combination with polyvinyl alcohol (PVA) in comparison with Ce6 alone and with the clinical drug, Photodithazine in a mouse tumor model. We also investigated the photoactivity of the Ce6-PVA in a model reaction of tryptophan oxidation, analyzed the polymer–Ce6 interaction using fluorescence spectroscopy and atomic-force microscopy, and tested the phototoxicity in vitro. Using fluorescence imaging in vivo we found that injection to mice of Ce6 in a formulation with PVA resulted in a higher tumor-to-normal ratio and gr