Optical and Electron Microscopic Study of the Morphology and Ultrastructure of Biofilms Formed by Streptococcus pyogenes
Danilova T. A., Smirnova T. A., Danilina G. A., Adzhieva A. A., Andreevskaya S. G., Shevlyagina N. V., Zhukhovitsky V. G.
Bulletin of Experimental Biology and Medicine
Vol.165, Issue1, P. 110-114
Опубликовано: 2018
Тип ресурса: Статья
DOI:10.1007/s10517-018-4110-1
Аннотация:
Our study confirmed the capacity of S. pyogenes strains to form biofilms on abiotic surfaces. Chains of streptococci surrounded by bluish film were seen under a microscope after alcian blue staining of the preparations grown on slides. On ultrathin sections in transmission electron microscope, the extracellular matrix (indicator of biofilm maturity) became visible after staining with alcian blue. Microscopy of the sections shows structures characteristic of a biofilm in spaces between the cells. Scanning electron microscopy also demonstrates the presence of a biomembrane. Importantly that type 1M strain forming in fact no membranes when cultured on plastic plates (Costar) formed biofilms on the glass. It seems that the conditions for the biofilm formation on the plastic and on the glass differ, due to which the exopolymeric matrices formed on different surfaces vary by biochemical composition. © 2018, Springer Science+Business Media, LLC, part of Springer Nature.
Ключевые слова:
biofilm; electron microscopy; light microscopy; matrix; S. pyogenes
alcian blue; bacterial polysaccharide; glass; plastic; Article; bacterial cell wall; bacterial growth; bacterial strain; bacterium culture; biochemical composition; biofilm; biomembrane; cell proliferation; controlled study; cytoplasm; electron microscopy; extracellular matrix; microscopy; nonhuman; protoplast; scanning electron microscopy; staining; Streptococcus pyogenes; transmission electron microscopy; ultrastructure; biofilm; growth, development and aging; microscopy; procedures; Streptococcus pyogenes; Biofilms; Microscopy; Microscopy, Electron, Scanning; Streptococcus pyogenes
Язык текста: Английский
ISSN: 1573-8221
Danilova T. A.
Smirnova T. A.
Danilina G. A.
Adzhieva A. A.
Andreevskaya S. G.
Shevlyagina N. V.
Zhukhovitsky V. G.
Данилова Т. А.
Смирнова Т. А.
Данилина Г. А.
Аджиева А. А.
Андреевскайа С. Г.
Шевлягина Н. В.
Жуховицкy В. Г.
Optical and Electron Microscopic Study of the Morphology and Ultrastructure of Biofilms Formed by Streptococcus pyogenes
Текст визуальный непосредственный
Bulletin of Experimental Biology and Medicine
Springer New York Consultants Bureau
Vol.165, Issue1 P. 110-114
2018
Статья
biofilm electron microscopy light microscopy matrix S. pyogenes
alcian blue bacterial polysaccharide glass plastic Article bacterial cell wall bacterial growth bacterial strain bacterium culture biochemical composition biofilm biomembrane cell proliferation controlled study cytoplasm electron microscopy extracellular matrix microscopy nonhuman protoplast scanning electron microscopy staining Streptococcus pyogenes transmission electron microscopy ultrastructure biofilm growth, development and aging microscopy procedures Streptococcus pyogenes Biofilms Microscopy Microscopy, Electron, Scanning Streptococcus pyogenes
Our study confirmed the capacity of S. pyogenes strains to form biofilms on abiotic surfaces. Chains of streptococci surrounded by bluish film were seen under a microscope after alcian blue staining of the preparations grown on slides. On ultrathin sections in transmission electron microscope, the extracellular matrix (indicator of biofilm maturity) became visible after staining with alcian blue. Microscopy of the sections shows structures characteristic of a biofilm in spaces between the cells. Scanning electron microscopy also demonstrates the presence of a biomembrane. Importantly that type 1M strain forming in fact no membranes when cultured on plastic plates (Costar) formed biofilms on the glass. It seems that the conditions for the biofilm formation on the plastic and on the glass differ, due to which the exopolymeric matrices formed on different surfaces vary by biochemical composition. © 2018, Springer Science+Business Media, LLC, part of Springer Nature.