Аннотация:
The purpose of the study - to determine the feasibility of using the succinate-based antioxidant for the in vitro reduction of excessive methemoglobin to oxyhemoglobin in blood. Materials and Methods. Blood sampling was performed in five healthy donors in microvettes containing EDTA during prophylactic examinations. NaNO2 solution was added to blood samples in vitro in order to yield methemoglobin (MetHb). The complex drug containing the following active ingredients: succinic acid, inosine, riboflavin, nicotinamide, was used as an antioxidant. The absorption spectrum of red cell suspensions with different drug content Dl(λl) exper was measured with 1 nm increments. The non-linear regression method was used to calculate concentrations of hemoglobin derivatives in suspensions. Results. In our experiments, when methemoglobin reacted with drug the optical density of peaks typical for oxyhemoglobin increased and the spectral peak of methemoglobin decreased. The greater the concentration of
Cherny'sh A. M. Aleksandr Mikhaylovich 1941-
Kozlova E. K. Elena Karlovna 1958-
Moroz V. V.
Sergunova V. A.
Gudkova O. E.
Sherstukova E. A. Ekaterina Aleksandrovna 1993-
Kozlov A. P. Aleksandr Pavlovich 1981-
Черныш А. М. Александр Михайлович 1941-
Козлова Е. К. Елена Карловна 1958-
Мороз В. В.
Сергунова В. А.
Гудкова О. Е.
Шерстюкова Е. А. Екатерина Александровна 1993-
Козлов А. П. Александр Павлович 1981-
Effects of succinate-based antioxidant on in vitro conversion of methemoglobin in oxyhemoglobin
Текст визуальный непосредственный
Общая реаниматология
Некоммерческая организация Фонд "Медицина критических состояний"
Vol.14, Issue2 P. 46-59
2018
Статья
Cytoflavin Inosine Methemoglobin Nicotinamide Oxyhemoglobin Red blood cells Reduction Riboflavin Succinic acid
The purpose of the study - to determine the feasibility of using the succinate-based antioxidant for the in vitro reduction of excessive methemoglobin to oxyhemoglobin in blood. Materials and Methods. Blood sampling was performed in five healthy donors in microvettes containing EDTA during prophylactic examinations. NaNO2 solution was added to blood samples in vitro in order to yield methemoglobin (MetHb). The complex drug containing the following active ingredients: succinic acid, inosine, riboflavin, nicotinamide, was used as an antioxidant. The absorption spectrum of red cell suspensions with different drug content Dl(λl) exper was measured with 1 nm increments. The non-linear regression method was used to calculate concentrations of hemoglobin derivatives in suspensions. Results. In our experiments, when methemoglobin reacted with drug the optical density of peaks typical for oxyhemoglobin increased and the spectral peak of methemoglobin decreased. The greater the concentration of