Synthetic analogue of leu-enkephalin prevents endothelial dysfunction in vitro
Grebenchikov O. A., Ovezov A. M., Skripkin Y. V., Zabelina T. S., Ulitkina O. N., Lugovoy A. V., Prikhodko A. S., Ryzhkov A. Y., Zinovkin R. A.
Общая реаниматология
Vol.14, Issue2, P. 60-68
Опубликовано: 2018
Тип ресурса: Статья
DOI:10.15360/1813-9779-2018-2-60-68
Аннотация:
The purpose of the study was to determine the efficacy of a synthetic leu-enkephalin stabilized analogue to prevent damage of endothelial cells monolayer in vitro caused by serum samples from septic shock patients. Materials and methods. The experiments were performed using the EaHy.926 endothelial cells monolayer. We studied the in vitro effect of synthetic leu-enkephalin analogue on the cell damage caused by serum samples from five septic shock patients. The status of endothelial intercellular junctions was estimated by immunofluorescence microscopy and western blot with antibodies against adherens junction protein, VE-cadherin, and against the tight junctions protein, claudin. Cell viability was determined by staining with propidium iodide. Results. Preconditioning with a synthetic leu-enkephalin analogue (10, 50 and 100 µg/ml) of endothelial cells in vitro prevented the destruction of both tight and adherens junction and partially prevented endothelial cell death. Conclusion. Preco
Ключевые слова:
Dalargin; Endothelial permeability; Septic shock; Synthetic leu-enkephalin stabilized analogue; Systemic inflammatory response syndrome
Язык текста: Английский
ISSN: 2411-7110
Grebenchikov O. A.
Ovezov A. M.
Skripkin Y. V.
Zabelina T. S.
Ulitkina O. N.
Lugovoy A. V.
Prikhodko A. S.
Ryzhkov A. Y.
Zinovkin R. A. Roman Alekseevich 1973-
Гребенчиков О. А.
Овезов А. М.
Скрипкин Y. В.
Забелина Т. С.
Улиткина О. Н.
Луговоy А. В.
Приходко А. С.
Рyжков А. Y.
Зиновкин Р. А. Роман Алексеевич 1973-
Synthetic analogue of leu-enkephalin prevents endothelial dysfunction in vitro
Текст визуальный непосредственный
Общая реаниматология
Некоммерческая организация Фонд "Медицина критических состояний"
Vol.14, Issue2 P. 60-68
2018
Статья
Dalargin Endothelial permeability Septic shock Synthetic leu-enkephalin stabilized analogue Systemic inflammatory response syndrome
The purpose of the study was to determine the efficacy of a synthetic leu-enkephalin stabilized analogue to prevent damage of endothelial cells monolayer in vitro caused by serum samples from septic shock patients. Materials and methods. The experiments were performed using the EaHy.926 endothelial cells monolayer. We studied the in vitro effect of synthetic leu-enkephalin analogue on the cell damage caused by serum samples from five septic shock patients. The status of endothelial intercellular junctions was estimated by immunofluorescence microscopy and western blot with antibodies against adherens junction protein, VE-cadherin, and against the tight junctions protein, claudin. Cell viability was determined by staining with propidium iodide. Results. Preconditioning with a synthetic leu-enkephalin analogue (10, 50 and 100 µg/ml) of endothelial cells in vitro prevented the destruction of both tight and adherens junction and partially prevented endothelial cell death. Conclusion. Preco