Penetrating cations induce pleiotropic drug resistance in yeast
Galkina K. V., Besedina E. G., Zinovkin R. A., Severin F. F., Knorre D. A.
Scientific Reports
Vol.8, Issue1, Num.8131
Опубликовано: 2018
Тип ресурса: Статья
DOI:10.1038/s41598-018-26435-z
Аннотация:
Substrates of pleiotropic drug resistance (PDR) transporters can induce the expression of corresponding transporter genes by binding to their transcription factors. Penetrating cations are substrates of PDR transporters and theoretically may also activate the expression of transporter genes. However, the accumulation of penetrating cations inside mitochondria may prevent the sensing of these molecules. Thus, whether penetrating cations induce PDR is unclear. Using Saccharomyces cerevisiae as a model, we studied the effects of penetrating cations on the activation of PDR. We found that the lipophilic cation dodecyltriphenylphosphonium (C12TPP) induced the expression of the plasma membrane PDR transporter genes PDR5, SNQ2 and YOR1. Moreover, a 1-hour incubation with C12TPP increased the concentration of Pdr5p and Snq2p and prevented the accumulation of the PDR transporter substrate Nile red. The transcription factor PDR1 was required to mediate these effects, while PDR3 was dispensable.
Ключевые слова:
DNA binding protein; dodecyltriphenylphosphonium; organophosphorus compound; PDR1 protein, S cerevisiae; PDR3 protein, S cerevisiae; Saccharomyces cerevisiae protein; transcription factor; antifungal resistance; cell nucleus; cytology; drug effect; gene deletion; genetics; metabolism; mitochondrion; pleiotropy; Saccharomyces cerevisiae; signal transduction; Cell Nucleus; DNA-Binding Proteins; Drug Resistance, Fungal; Gene Deletion; Genetic Pleiotropy; Mitochondria; Organophosphorus Compounds; Saccharomyces cerevisiae; Saccharomyces cerevisiae Proteins; Signal Transduction; Transcription Factors
Язык текста: Английский
ISSN: 2045-2322
Galkina K. V.
Besedina E. G.
Zinovkin R. A. Roman Alekseevich 1973-
Severin F. F.
Knorre D. A. Dmitrij Alekseevich 1979-
Галкина К. В.
Беседина Е. Г.
Зиновкин Р. А. Роман Алексеевич 1973-
Северин Ф. Ф.
Кнорре Д. А. Дмитрий Алексеевич 1979-
Penetrating cations induce pleiotropic drug resistance in yeast
Текст визуальный непосредственный
Scientific Reports
Vol.8, Issue1 Num.8131
2018
Статья
DNA binding protein dodecyltriphenylphosphonium organophosphorus compound PDR1 protein, S cerevisiae PDR3 protein, S cerevisiae Saccharomyces cerevisiae protein transcription factor antifungal resistance cell nucleus cytology drug effect gene deletion genetics metabolism mitochondrion pleiotropy Saccharomyces cerevisiae signal transduction Cell Nucleus DNA-Binding Proteins Drug Resistance, Fungal Gene Deletion Genetic Pleiotropy Mitochondria Organophosphorus Compounds Saccharomyces cerevisiae Saccharomyces cerevisiae Proteins Signal Transduction Transcription Factors
Substrates of pleiotropic drug resistance (PDR) transporters can induce the expression of corresponding transporter genes by binding to their transcription factors. Penetrating cations are substrates of PDR transporters and theoretically may also activate the expression of transporter genes. However, the accumulation of penetrating cations inside mitochondria may prevent the sensing of these molecules. Thus, whether penetrating cations induce PDR is unclear. Using Saccharomyces cerevisiae as a model, we studied the effects of penetrating cations on the activation of PDR. We found that the lipophilic cation dodecyltriphenylphosphonium (C12TPP) induced the expression of the plasma membrane PDR transporter genes PDR5, SNQ2 and YOR1. Moreover, a 1-hour incubation with C12TPP increased the concentration of Pdr5p and Snq2p and prevented the accumulation of the PDR transporter substrate Nile red. The transcription factor PDR1 was required to mediate these effects, while PDR3 was dispensable.