Imaging of oxygen and hypoxia in cell and tissue samples
Papkovsky D. B., Dmitriev R. I.
Cellular and Molecular Life Sciences (CMLS)
Vol.75, Issue16, P. 2963-2980
Опубликовано: 2018
Тип ресурса: Обзор
DOI:10.1007/s00018-018-2840-x
Аннотация:
Molecular oxygen (O 2 ) is a key player in cell mitochondrial function, redox balance and oxidative stress, normal tissue function and many common disease states. Various chemical, physical and biological methods have been proposed for measurement, real-time monitoring and imaging of O 2 concentration, state of decreased O 2 (hypoxia) and related parameters in cells and tissue. Here, we review the established and emerging optical microscopy techniques allowing to visualize O 2 levels in cells and tissue samples, mostly under in vitro and ex vivo, but also under in vivo settings. Particular examples include fluorescent hypoxia stains, fluorescent protein reporter systems, phosphorescent probes and nanosensors of different types. These techniques allow high-resolution mapping of O 2 gradients in live or post-mortem tissue, in 2D or 3D, qualitatively or quantitatively. They enable control and monitoring of oxygenation conditions and their correlation with other biomarkers of cell and tiss
Ключевые слова:
FLIM; Fluorescence and phosphorescence-based probes; Fluorescence microscopy; Hypoxia; Live cell and tissue imaging; Oxygen microscopy; PLIM
oxygen; fluorescent dye; nanomaterial; oxygen; photoprotein; cell function; cell hypoxia; cell level; correlational study; electrochemistry; electron spin resonance; ex vivo study; fluorescence microscopy; human; hypoxemia; in vitro study; molecular imaging; nonhuman; oxygen tissue level; oxygenation; photoacoustic spectroscopy; qualitative analysis; quantitative analysis; Raman spectrometry; Review; staining; animal; brain; chemistry; fluorescence imaging; genetics; hypoxia; metabolism; pathology; Animals; Brain; Fluorescent Dyes; Humans; Hypoxia; Luminescent Proteins; Microscopy, Fluorescence; Nanostructures; Optical Imaging; Oxygen
Язык текста: Английский
ISSN: 1420-9071
Papkovsky D. B.
Dmitriev R. I. Ruslan Igorevich 1981-
Папковскy Д. Б.
Дмитриев Р. И. Руслан Игоревич 1981-
Imaging of oxygen and hypoxia in cell and tissue samples
Текст визуальный непосредственный
Cellular and Molecular Life Sciences (CMLS)
Birkhäuser Verlag AG
Vol.75, Issue16 P. 2963-2980
2018
Обзор
FLIM Fluorescence and phosphorescence-based probes Fluorescence microscopy Hypoxia Live cell and tissue imaging Oxygen microscopy PLIM
oxygen fluorescent dye nanomaterial oxygen photoprotein cell function cell hypoxia cell level correlational study electrochemistry electron spin resonance ex vivo study fluorescence microscopy human hypoxemia in vitro study molecular imaging nonhuman oxygen tissue level oxygenation photoacoustic spectroscopy qualitative analysis quantitative analysis Raman spectrometry Review staining animal brain chemistry fluorescence imaging genetics hypoxia metabolism pathology Animals Brain Fluorescent Dyes Humans Hypoxia Luminescent Proteins Microscopy, Fluorescence Nanostructures Optical Imaging Oxygen
Molecular oxygen (O 2 ) is a key player in cell mitochondrial function, redox balance and oxidative stress, normal tissue function and many common disease states. Various chemical, physical and biological methods have been proposed for measurement, real-time monitoring and imaging of O 2 concentration, state of decreased O 2 (hypoxia) and related parameters in cells and tissue. Here, we review the established and emerging optical microscopy techniques allowing to visualize O 2 levels in cells and tissue samples, mostly under in vitro and ex vivo, but also under in vivo settings. Particular examples include fluorescent hypoxia stains, fluorescent protein reporter systems, phosphorescent probes and nanosensors of different types. These techniques allow high-resolution mapping of O 2 gradients in live or post-mortem tissue, in 2D or 3D, qualitatively or quantitatively. They enable control and monitoring of oxygenation conditions and their correlation with other biomarkers of cell and tiss