Аннотация:
The complex of mitochondrial protein cytochrome c (CytC) with anionic phospholipid cardiolipin (CL) plays a crucial role in the initiation of apoptosis by catalyzing lipid peroxidation in mitochondrial membranes. In our previous papers, we found that CytC and CL mixed in millimolar concentrations form a sediment showing microcrystals composed of nanospheres (Cyt-CL) of 11–12 and 8 nm in diameter. The hypothesis was proposed that Cyt-CL, having hydrophobic shell, may appear inside the membrane lipid bilayer in mitochondria and peroxidase membrane phospholipids so initiating the apoptotic cascade. In this work, Cyt-CL complex dissolved in chloroform or hexane was investigated as a model of the complex in mitochondrial membranes. We used dynamic light scattering method to measure the size of the particles. The analysis of particles size distribution of Cyt-CL in chloroform allows to reveal three dominant diameters of 12.1 ± 1.4, 7.8 ± 1.0, and 4.7 ± 0.7 nm. The first two values are closed
Ключевые слова:
Cardiolipin; Chemiluminescence; Cytochrome c; Dynamic light scattering
cardiolipin; chloroform; coumarin; cytochrome c; hexane; lipid peroxidase; liposome; lipoxygenase; membrane lipid; membrane phospholipid; methanol; peroxy radical; tryptophan; tyrosine; water; cardiolipin; cytochrome c; nanosphere; peroxidase; solvent; Article; chemoluminescence; complex formation; concentration (parameters); conformational transition; crystal; enzyme activity; hydrophobicity; lipid bilayer; lipid peroxidation; mitochondrial membrane; particle size; photon correlation spectroscopy; priority journal; protein conformation; protein structure; sediment; solvent effect; spectrofluorometry; X ray crystallography; chemical phenomena; chemistry; luminescence; metabolism; small angle scattering; X ray diffraction; Cardiolipins; Chloroform; Cytochromes c; Dynamic Light Scattering; Hydrophobic and Hydrophilic Interactions; Lipoxygenase; Luminescent Measurements; Methanol; Nanospheres; Particle Size; Peroxidase; Scattering, Small Angle; Solvents; Spectrometry, Fluorescence; X-Ray
Vladimirov G. K. Georgij Konstantinovich 1992-
Vikulina A. S.
Volodkin D.
Vladimirov Yu. A. Yurij Andreevich 1932-
Владимиров Г. К. Георгий Константинович 1992-
Викулина А. С.
Володкин Д.
Владимиров Ю. А. Юрий Андреевич 1932-
Structure of the complex of cytochrome c with cardiolipin in non-polar environment
Текст визуальный непосредственный
Chemistry and Physics of Lipids
Elsevier Science Publisher B.V.
Vol.214 P. 35-45
2018
Статья
Cardiolipin Chemiluminescence Cytochrome c Dynamic light scattering
cardiolipin chloroform coumarin cytochrome c hexane lipid peroxidase liposome lipoxygenase membrane lipid membrane phospholipid methanol peroxy radical tryptophan tyrosine water cardiolipin cytochrome c nanosphere peroxidase solvent Article chemoluminescence complex formation concentration (parameters) conformational transition crystal enzyme activity hydrophobicity lipid bilayer lipid peroxidation mitochondrial membrane particle size photon correlation spectroscopy priority journal protein conformation protein structure sediment solvent effect spectrofluorometry X ray crystallography chemical phenomena chemistry luminescence metabolism small angle scattering X ray diffraction Cardiolipins Chloroform Cytochromes c Dynamic Light Scattering Hydrophobic and Hydrophilic Interactions Lipoxygenase Luminescent Measurements Methanol Nanospheres Particle Size Peroxidase Scattering, Small Angle Solvents Spectrometry, Fluorescence X-Ray
The complex of mitochondrial protein cytochrome c (CytC) with anionic phospholipid cardiolipin (CL) plays a crucial role in the initiation of apoptosis by catalyzing lipid peroxidation in mitochondrial membranes. In our previous papers, we found that CytC and CL mixed in millimolar concentrations form a sediment showing microcrystals composed of nanospheres (Cyt-CL) of 11–12 and 8 nm in diameter. The hypothesis was proposed that Cyt-CL, having hydrophobic shell, may appear inside the membrane lipid bilayer in mitochondria and peroxidase membrane phospholipids so initiating the apoptotic cascade. In this work, Cyt-CL complex dissolved in chloroform or hexane was investigated as a model of the complex in mitochondrial membranes. We used dynamic light scattering method to measure the size of the particles. The analysis of particles size distribution of Cyt-CL in chloroform allows to reveal three dominant diameters of 12.1 ± 1.4, 7.8 ± 1.0, and 4.7 ± 0.7 nm. The first two values are closed